Investigation of Trace Elements in Vegan Foods by ICP-MS After Microwave Digestion

Köprü, Semiha; Çadır, Mehmet; Soylak, Mustafa

doi:10.1007/s12011-022-03106-9

Cited by 13 publications

(6 citation statements)

References 51 publications

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“…Inadequate mineral intake is not limited to individuals consuming a western style diet. A recent study demonstrated that a majority of individuals in many developing regions of the world also lacked an adequate amount of calcium in their diet (75,76). Whether the mineral supplement used here or some other formulation might provide a way to insure adequate mineral intake remains to be seen.…”

Section: Discussionmentioning

confidence: 92%

Liver Protein Expression in Nash Mice on a High-Fat Diet

Varani

McClintock

Knibbs

et al. 2022

Preprint

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Male MS-NASH mice were maintained on a high-fat diet for 16 weeks with and without red algae-derived minerals. Obeticholic acid (OCA) was used as a comparator in the same strain and diet. C57BL/6 mice maintained on a standard (low-fat) rodent chow diet were used as a control. At the end of the in-life portion of the study, body weight, liver weight, liver enzyme levels and liver histology were assessed. Samples obtained from individual livers were subjected to Tandem Mass Tag labeling/mass spectroscopy for protein profile determination. As compared to mice maintained on the low-fat diet, all high-fat-fed mice had increased whole body and liver weight, increased liver enzyme (aminotransferases) levels and widespread steatosis/ballooning hepatocyte degeneration. Histological evidence for liver inflammation and collagen deposition was also present, but changes were to a lesser extent. A moderate reduction in ballooning degeneration and collagen deposition was observed with mineral supplementation. Control mice on the high-fat diet alone demonstrated multiple protein changes associated with dysregulated fat and carbohydrate metabolism, lipotoxicity and oxidative stress. Cholesterol metabolism and bile acid formation were especially sensitive to diet. In mice receiving multi-mineral supplementation along with the high-fat diet, there was reduced liver toxicity as evidenced by a decrease in levels of several cytochrome P450 enzymes and other oxidant-generating moieties. Additionally, elevated expression of several keratins was also detected in mineral-supplemented mice. The protein changes observed with mineral supplementation were not seen with OCA. Our previous studies have shown that mice maintained on a high-fat diet for up to 18 months develop end-stage liver injury including hepatocellular carcinoma. Mineral-supplemented mice were substantially protected against tumor formation and other end-state consequences of high-fat feeding. The present study identifies early (16-week) protein changes occurring in the livers of the high-fat diet-fed mice, and how the expression of these proteins is influenced by mineral supplementation. These findings help elucidate early protein changes that contribute to end-stage liver injury and potential mechanisms by which dietary minerals may mitigate such damage.

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Section: Discussionmentioning

confidence: 92%

Liver Protein Expression in Nash Mice on a High-Fat Diet

Varani

McClintock

Knibbs

et al. 2022

Preprint

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show abstract

“…Nowadays, microwave‐assisted digestion is widely accepted as one of the most useful pretreatment techniques for food matrices [7–16, 20, 21]. Among the various reagents employed in microwave‐assisted digestion, concentrated HNO 3 is preferentially used due to its high purity and broad oxidative ability.…”

Section: Resultsmentioning

confidence: 99%

“…To date, various analytical techniques, such as inductively coupled plasma-mass spectrometry (ICP-MS) [7][8][9][10], inductively coupled plasma-optical emission spectrometry (ICP-OES) [11][12][13], and flame/electrothermal atomic absorption spectrometry (AAS) [14][15][16], have been used for the determination of metals in food. ICP-MS and ICP-OES are widely used due to their superior features (e.g., simultaneous multielement determination, extraordinary analytical sensitivity, and so on) [17,18]; however, the instrument procurement/running cost are quite expensive and require highly skilled instrument operators.…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of metallic additives in century eggs by microwave plasma‐atomic emission spectrometer and microwave‐assisted digestion

Chen,

Weng,

Wang

et al. 2024

J Chinese Chemical Soc

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Century eggs (also known as pídàn) are a widely used preserved food product in Taiwanese cuisine. Due to their peculiar appearance, pungent odor, and unusual texture, several consumers have raised concerns. Actually, some metallic additives are employed to control the quality of gelation of protein during the production of century eggs. Therefore, a method involving microwave plasma‐atomic emission spectrometer and microwave‐assisted digestion was proposed in this study. In addition, to clarify distribution information of metallic additives in century eggs, detailed analyses based on the egg structure were adopted. The linearities for Fe and Zn were obtained in the range of 0.1–5, and 0.02–1 mg L−1 for Cu and Pb. The method detection limits for test metals were 0.06–0.036 mg L−1. The measured values for the RM EGGS‐1 sample agreed reasonably well with the reference values, demonstrating that the developed method is practical for routine analysis with lower instrument procurement/running cost. The analytical results for century eggs collected from local markets revealed diverse distributions of metal ions in different parts. Remarkably, this method can readily identify the metallic additives used during the production processes even in the presence of complicated matrices.

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“…Inadequate mineral intake is not limited to individuals consuming a Western-style diet. A recent study demonstrated that a majority of individuals in many developing regions of the world also lacked an adequate amount of calcium in their diet ( 83 , 84 ). Whether the mineral supplement used here or some other formulation might provide a way to ensure adequate mineral intake remains to be seen.…”

Section: Discussionmentioning

confidence: 99%

Liver Protein Expression in NASH Mice on a High-Fat Diet: Response to Multi-Mineral Intervention

et al. 2022

View full text Add to dashboard Cite

Male MS-NASH mice were maintained on a high-fat diet for 16 weeks with and without red algae-derived minerals. Obeticholic acid (OCA) was used as a comparator in the same strain and diet. C57BL/6 mice maintained on a standard (low-fat) rodent chow diet were used as a control. At the end of the in-life portion of the study, body weight, liver weight, liver enzyme levels and liver histology were assessed. Samples obtained from individual livers were subjected to Tandem Mass Tag labeling / mass spectroscopy for protein profile determination. As compared to mice maintained on the low-fat diet, all high-fat-fed mice had increased whole-body and liver weight, increased liver enzyme (aminotransferases) levels and widespread steatosis / ballooning hepatocyte degeneration. Histological evidence for liver inflammation and collagen deposition was also present, but changes were to a lesser extent. A moderate reduction in ballooning degeneration and collagen deposition was observed with mineral supplementation. Control mice on the high-fat diet alone demonstrated multiple protein changes associated with dysregulated fat and carbohydrate metabolism, lipotoxicity and oxidative stress. Cholesterol metabolism and bile acid formation were especially sensitive to diet. In mice receiving multi-mineral supplementation along with the high-fat diet, there was reduced liver toxicity as evidenced by a decrease in levels of several cytochrome P450 enzymes and other oxidant-generating moieties. Additionally, elevated expression of several keratins was also detected in mineral-supplemented mice. The protein changes observed with mineral supplementation were not seen with OCA. Our previous studies have shown that mice maintained on a high-fat diet for up to 18 months develop end-stage liver injury including hepatocellular carcinoma. Mineral-supplemented mice were substantially protected against tumor formation and other end-state consequences of high-fat feeding. The present study identifies early (16-week) protein changes occurring in the livers of the high-fat diet-fed mice, and how the expression of these proteins is influenced by mineral supplementation. These findings help elucidate early protein changes that contribute to end-stage liver injury and potential mechanisms by which dietary minerals may mitigate such damage.

show abstract

Investigation of Trace Elements in Vegan Foods by ICP-MS After Microwave Digestion

Cited by 13 publications

References 51 publications

Liver Protein Expression in Nash Mice on a High-Fat Diet

Liver Protein Expression in Nash Mice on a High-Fat Diet

Rapid determination of metallic additives in century eggs by microwave plasma‐atomic emission spectrometer and microwave‐assisted digestion

Liver Protein Expression in NASH Mice on a High-Fat Diet: Response to Multi-Mineral Intervention

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