Investigation of TIMP2 Gene Polymorphisms Incidence in Patients with Breast Cancer

Abstract

The breast cancers were placed in first rank among other cancer types in women, and second rank after lung cancer in terms of death in the World. It is estimated that this cancer has 20/100.000 frequency in east region and 40-50/100.000 frequency west region according to present data in Turkey. Matrix Metalloproteinase (MMP), proteolytic enzymes involve in the breakdown of proteins located in the cell membrane and intercellular matrix such as collagen, elastin, proteoglycans, and gelatin. The tissue inhibitors of metalloproteinases (TIMPs) are proteins which are specific and natural inhibitor for MMPs. Its primary tasks regulate the activation of MMPs by suppress. The destruction of the balance between MMPs and TIMPs, may lead the emergence of pathological processes. In this study, the effect of TIMP2 (-418) G/C andTIMP2 (303) G/A polymorphisms in breast cancer patients in Turkish population were analyzed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).The statistically significant difference was found between the control group and patients with breast cancer. However it is found that TIMP2 (303) GA genotype increases the risk of developing breast cancer at 1.486fold (p=0519), TIMP2 (-418) CC genotype increases the risk of developing breast cancer at 3. 719-fold (p=0.519). In our knowledge, this study is the first to evaluate the relationship between breast cancer and TIMP2 gene polymorphisms in Turkish population. Our results show that there is no any association between breast cancer and TIMP2 gene polymorphisms in the community which is represented by our study and control groups. It was concluded from results that TIMP2 (303) GA genotype ile TIMP2 (-418) CC genotype may create the susceptibility to breast cancer.