Investigation of tigecycline bactericidal activity: Optimisation of laboratory testing

Usacheva, Elena A.; Grayes, Althea; Schora, Donna; Peterson, Lance R.

doi:10.1016/j.jgar.2014.04.005

Cited by 5 publications

(6 citation statements)

References 25 publications

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“…Although complete elimination of the S. mutans population was not obtained, this was promising as it represented a 2.86-Log reduction. Generally, when comparing the bactericidal activity of antimicrobials against bacteria, a 3-Log (99.9%) reduction is considered to be significant; the reduction obtained here was, therefore, considered promising (Usacheva et al, 2014, Heffernan et al, 2013, Koh et al, 2013, Sun et al, 2014.…”

Section: Discussionmentioning

confidence: 81%

The potential of Streptococcus salivarius oral films in the management of dental caries: An inkjet printing approach

Dodoo

Stapleton

Basit

et al. 2020

International Journal of Pharmaceutics

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Oral infections like dental caries are major public health issues globally. Management of these conditions include methods like brushing, tongue cleaning and dental flossing with or without the use of antibiotics. A major challenge with these management approaches is the difficulty to obtain complete eradication of the disease-causing organisms. An alternative approach is the use of probiotics, which can be administered in oro-dispersible films (ODFs), and which have prolonged activity in the mouth. ODFs made of xylitol and containing Streptococcus salivarius were formulated using inkjet printing and tested against Streptococcus mutansa causative organism of dental caries. The testing of the prepared ODFs involved co-incubating ink-jetted formulation of S. salivarius and xylitol with S. mutans and monitoring the microbial growth kinetics in real-time using isothermal microcalorimetry and colony plate counts. Cell-free supernatants (CFS) of S. salivarius were also tested against S. mutans. Prior to the formulation the phosphate solubilisation potential of S. salivarius was determined and found to be negative, a sign that the species will not deplete phosphate from teeth. From the tests, it was observed that the formulation reduced S. mutans population from 7.9 to 5.04-Log CFU/mL post-calorimetry (approximately 3-Log reduction) which was comparable to the 99.9% reduction expected during antimicrobial activity testing. A gradual decrease in S. mutans population was also observed with increasing of S. salivarius CFS volumes indicative of pathogen suppression. This study demonstrates that S. salivarius can be useful in managing dental caries and ODFs of S. salivarius can be formulated easily using ink-jetting for such management.

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Section: Discussionmentioning

confidence: 81%

The potential of Streptococcus salivarius oral films in the management of dental caries: An inkjet printing approach

Dodoo

Stapleton

Basit

et al. 2020

International Journal of Pharmaceutics

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“…In this case, however, colony counts at 48h ( Figure 6) show that the pathogen has not been completely eradicated; its numbers reduced to 10 4 cfu/mL. However, in evaluating antimicrobial effects, a 3-log reduction is considered to be a significant reduction (Usacheva et al, 2014, Heffernan et al, 2013, Koh et al, 2013. S. sonnei has been reported to be very susceptible to acidic environments.…”

Section: Resultsmentioning

confidence: 98%

Use of a water-based probiotic to treat common gut pathogens

Dodoo

Stapleton

Basit

et al. 2019

International Journal of Pharmaceutics

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This work reports the anti-pathogenic effect of a commercially available water-based probiotic suspension, Symprove, against three commonly encountered infectious organisms; Escherichia coli, methicillin-resistant Staphylococcus aureus (MRSA) and Shigella sonnei. An isothermal calorimetric assay was used to the monitor growth of the species individually and in binary combinations, while colony plate counting was used to enumerate viable cell numbers. It was observed that all pathogenic species were faster growing than the probiotic bacteria in Symprove after inoculation into growth medium yet in all instances bacterial enumeration at the end of the experiments revealed a significant reduction in the pathogen population compared with the controls. A control population between 10 8-10 9 CFU/ml was obtained for E. coli and S. sonnei whilst approximately 10 6 CFU/ml was obtained for MRSA. Upon co-incubation for 48 hours, no viable counts were obtained for E. coli; a 4-log reduction was obtained for S. sonnei whilst MRSA numbers were down to less than 10 cells/ml. The results show that Symprove has antipathogenic activity against E. coli, S. sonnei and MRSA.

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“…Previously, CFU measurements revealed that prolonged exposure to TGC exerted a bactericidal effect against various bacteria in vitro [47] and in vivo. [48] Considering the rapid bactericidal-like response in some bacteria that were recorded by our protocol, further exploration of a novel mechanism of TGC, a highly potent drug in treating multidrug-resistant Gram-negative bacteria, is imperative. [49] Of note, understanding the bacterial metabolic states in response to antibiotic treatment is important.…”

Section: Discussionmentioning

confidence: 99%

d‐Amino Acid‐Based Metabolic Labeling Enables a Fast Antibiotic Susceptibility Test of Both Isolated Bacteria and Bronchoalveolar Lavage Fluid

Gao

Guo

Chen

et al. 2021

Adv Healthcare Materials

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The threat of multidrug-resistant bacteria has escalated rapidly, increasing the demand for accurate antibiotic susceptibility tests (ASTs). Traditional bacterial growth yield-based ASTs often take overnight to report, delaying the timely guidance of antibiotic use. Here, a fluorescent d-amino acid (FDAA) labeling-based AST (FaAST) is reported, which can quickly provide accurate minimum inhibitory concentrations (MICs). The FDAA-labeling signals that reflect the bacterial metabolic status underlie the flow cytometry-based strategy for MIC determination. Resistant bacteria show a reluctant decline in FDAA-labeling (inhibited metabolism) after treatment with the corresponding antibiotics, whereas susceptible bacteria demonstrate quick responses to low doses of drugs. The MICs are determined based on the changing trends in labeling. After testing 23 clinical isolates and laboratory strains of the most critical drug-resistant bacteria against a panel of representative antibiotics, FaAST shows a high susceptibility category with an accuracy of 98.13%. Moreover, FaAST can also make quick and accurate diagnosis against bronchoalveolar lavage fluids collected from hospital-acquired pneumonia patients, saving 2-4 days in guiding antibiotic use for this life-threatening infection. Thus, the speed, accuracy, and broad applicability of FaAST will be valuable in informing antibiotic decisions when treating critical infections caused by drug-resistant bacteria.

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Investigation of tigecycline bactericidal activity: Optimisation of laboratory testing

Cited by 5 publications

References 25 publications

The potential of Streptococcus salivarius oral films in the management of dental caries: An inkjet printing approach

The potential of Streptococcus salivarius oral films in the management of dental caries: An inkjet printing approach

Use of a water-based probiotic to treat common gut pathogens

d‐Amino Acid‐Based Metabolic Labeling Enables a Fast Antibiotic Susceptibility Test of Both Isolated Bacteria and Bronchoalveolar Lavage Fluid

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