Investigation of the synergistic effect of platelet-rich plasma and polychromatic light on human dermal fibroblasts seeded chitosan/gelatin scaffolds for wound healing

Koyuncu, Ayfer; Koç, Sena; Akdere, Özge Ekin; Çakmak, Anıl Sera; Gümüşderelı́oğlu, Menemşe

doi:10.1016/j.jphotobiol.2022.112476

Cited by 9 publications

(5 citation statements)

References 50 publications

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“…Thus, fibroblasts were chemically and physically stimulated by PRP and light, respectively. It was found that the expression of laminin and collagen 4 was up-regulated, and the angiogenesis related PDGF and VEGF were also significantly increased under the action of PRP and light (Koyuncu et al, 2022). However, this complex system also means that biomaterials have also changed from single to complex, from the combination of simple biomaterials and some ingredients that promote wound healing to a bionic system that strives to restore the normal wound healing mode (Bulutoglu et al, 2022).…”

Section: Ph Sensitivitymentioning

confidence: 99%

Effect of composite biodegradable biomaterials on wound healing in diabetes

Ren

Guo²,

Yang³

et al. 2022

Front. Bioeng. Biotechnol.

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The repair of diabetic wounds has always been a job that doctors could not tackle quickly in plastic surgery. To solve this problem, it has become an important direction to use biocompatible biodegradable biomaterials as scaffolds or dressing loaded with a variety of active substances or cells, to construct a wound repair system integrating materials, cells, and growth factors. In terms of wound healing, composite biodegradable biomaterials show strong biocompatibility and the ability to promote wound healing. This review describes the multifaceted integration of biomaterials with drugs, stem cells, and active agents. In wounds, stem cells and their secreted exosomes regulate immune responses and inflammation. They promote angiogenesis, accelerate skin cell proliferation and re-epithelialization, and regulate collagen remodeling that inhibits scar hyperplasia. In the process of continuous combination with new materials, a series of materials that can be well matched with active ingredients such as cells or drugs are derived for precise delivery and controlled release of drugs. The ultimate goal of material development is clinical transformation. At present, the types of materials for clinical application are still relatively single, and the bottleneck is that the functions of emerging materials have not yet reached a stable and effective degree. The development of biomaterials that can be further translated into clinical practice will become the focus of research.

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Section: Ph Sensitivitymentioning

confidence: 99%

Effect of composite biodegradable biomaterials on wound healing in diabetes

Ren

Guo²,

Yang³

et al. 2022

Front. Bioeng. Biotechnol.

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“…In vitro studies have shown that FN polymerization leads to the composition and stability of the extracellular matrix and cellmatrix adhesion 7,29 . FN polymerization enables the accumulation of COL types I and III in the extracellular matrix and results in the stabilization of COLI matrix fibrils.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the İn Vitro Effect of Vanillic Acid on Wound Healing via FN1 and COL1α1 Genes

Yeni

2024

Dicle Tıp Dergisi

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Objective: Wound healing is characterized by the removal of dead/damaged tissue, the formation of new tissue, and finally, the restoration of the damaged tissue to its original function, starting from the moment of tissue injury. Vanillic acid (VA) is an important component of wheat bran and can heal wounds thanks to its antioxidant potential. This work aimed to investigate the dose-dependent effects of VA (1-2-4-8-16 and 32 μg/ml) in an in vitro way using a wound healing pattern in fibroblast cells. Methods: The MTT test was performed to determine cell viability 48 hours after VA application to the cells in which the wound model was created (except for the control and wound groups). The cells were examined morphologically with an inverted microscope. ELISA and Real-Time PCR analyses were performed to determine changes in oxidative stress parameters and FN1 and COL1α1 gene expressions. Results: The highest percentage closure rate of fibroblast cells in the in vitro wound pattern analysis and the highest percentage of cell viability by MTT analysis were determined in the VA-32 treated group. Morphological images showed that the evaluated gene expressions increased in fibroblast cells in a VA dose-dependent manner. Conclusion: Our findings demonstrated for the first time that VA promotes cell migration and proliferation by regulating oxidative stress and FN1A and COL1α1 genes. The results of this work are thought to pioneer the use of VA in in vivo wound healing studies.

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“…In addition, fibronectin can link to other cells to further stabilize the ECM [27]. In vitro studies have shown that Fibronectin polymerization leads to the composition and stability of the ECM and cell matrix adhesion [27,28]. Fibronectin polymerization enables collagen types I and III to be deposited into the ECM and results in stabilization of collagen I matrix fibrils11.…”

Section: Discussionmentioning

confidence: 99%

Wound Healing of Quinic Acid in Human Dermal Fibroblasts by Regulating Expression of FN1 and COL1A1 Gene

Genç

Yeni

Çiçek

et al. 2022

Türk Doğa Ve Fen Dergisi

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Quinic acid (QA) is an alicyclic organic acid widely found in plants. It accumulates in varying concentrations of plant species and is actively metabolized throughout the plant's life cycle. Wound healing after skin injury involves a complex interaction of many cells, fibroblasts, endothelial cells, and regenerated immune cells and their interrelating extracellular matrix. In our study, the healing effect of QA on scar tissue was studied. For this aim, oxidative stress, and changes in FN1 and Collogen1α gene levels were examined. For this purpose, fibroblast cells were seeded in 24, 96 and well plates for wound healing, MTT analysis and Real-Time PCR testing (respectively). Wells were drawn with a 100 µL pipette tip for wound line. As a conclusion of our study, it was determined that cell viability increased significantly, especially in the QA 20 µg-ml group at the end of 48 hours. Increased cell viability and antioxidant capacity resulted in increased cell proliferation. Both FN1 and COL1A1 gene expression levels were up regulated in the QA groups compared to the control group. Our findings show for the first time that quinic acid promotes migration and/or proliferation of fibroblasts by regulating oxidative stress and the FN1A and COL1A1 genes. This activity may be related to the production of FN1A and COL1A1, which are considered important targets for modulation of the tissue repair process.

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Investigation of the synergistic effect of platelet-rich plasma and polychromatic light on human dermal fibroblasts seeded chitosan/gelatin scaffolds for wound healing

Cited by 9 publications

References 50 publications

Effect of composite biodegradable biomaterials on wound healing in diabetes

Effect of composite biodegradable biomaterials on wound healing in diabetes

Investigation of the İn Vitro Effect of Vanillic Acid on Wound Healing via FN1 and COL1α1 Genes

Wound Healing of Quinic Acid in Human Dermal Fibroblasts by Regulating Expression of FN1 and COL1A1 Gene

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