Investigation of the stability of Novozym® 435 in the production of biodiesel

José, Carla; Austic, Gregory B.; Bonetto, Rita Dominga; Burton, Rachel; Briand, Laura E.

doi:10.1016/j.cattod.2013.02.013

Cited by 30 publications

(19 citation statements)

References 26 publications

(41 reference statements)

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“…However, according to the preliminary experiments, using the immobilized form of the CALB (Novozym ® 435), there is a strong enzymatic deactivation due to the synergy between acid concentration and temperature. For this reason, we have proposed a kinetic model involving irreversible deactivation that obeys both Equations (13) and (14), with the reaction rate being of partial order 1 with respect to the concentration of ibuprofen and to the remaining activity.…”

Section: Kinetic Modellingmentioning

confidence: 99%

“…Novozym ® 435 is a commercially available heterogeneous biocatalyst Candida antarctica lipase B. This enzyme preparation has been used in the esterification of profens such as naproxen [7], ibuprofen [8,9], flurbiprofen [10], and ketoprofen [11] for the synthesis of prodrugs; it has also been used in the production of biodiesel [12][13][14] and in the development of polymerization bioprocesses [15][16][17]. It can actuate when suspended in various solvents and renewable resources such as supercritical carbon dioxide, ionic liquids [18], 2 of 19 glycerol [19][20][21], and deep eutectic solvents (DES), the high viscosity of which, as in the case of glycerol, can be reduced by water addition [22].…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Ibuprofen Monoglyceride in Solventless Medium with Novozym®435: Kinetic Analysis

et al. 2020

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This study investigates the enzymatic esterification of glycerol and ibuprofen in a solventless medium catalyzed by immobilized lipase B from Candida antarctica (Novozym®435). Fixing the concentration of this enzymatic solid preparation at 30 g·L−1, and operating at a constant stirring speed of 720 rpm, the temperature was changed between 50 and 80 °C, while the initial concentration of ibuprofen was studied from 20 to 100 g·L−1. Under these conditions, the resistance of external mass transport can be neglected, as confirmed by the Mears criterion (Me < 0.15). However, the mass transfer limitation inside the pores of the support has been evidenced. The values of the effectiveness factor (η) vary between 0.08 and 0.16 for the particle size range considered according to the Weisz–Prater criteria. Preliminary runs permit us to conclude that the enzyme was deactivated at medium to high temperatures and initial concentration values of ibuprofen. Several phenomenological kinetic models were proposed and fitted to all data available, using physical and statistical criteria to select the most adequate model. The best kinetic model was a reversible sigmoidal model with pseudo-first order with respect to dissolved ibuprofen and order 2 with respect to monoester ibuprofen, assuming the total first-order one-step deactivation of the enzyme, with partial first order for ibuprofen and enzyme activity.

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Section: Kinetic Modellingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synthesis of Ibuprofen Monoglyceride in Solventless Medium with Novozym®435: Kinetic Analysis

et al. 2020

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“…The band centered in 1732 cm −1 can be related to the stretching vibration of carbonyl groups ν(C=O) of the PMMA [94]. The bands at 1452 and 1512 cm −1 confirm the presence of DVB in the structure of the biocatalyst since it can be associated with the bond stretching between carbon atoms in the aromatic ring [95]. Moreover, the bands observed from 869 to 682 cm −1 are characteristic of the C-H out-of-plane bending of alkyl-substituted benzene.…”

Section: Modification Of the External And Internal Texture Of Biocatamentioning

confidence: 81%

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

et al. 2020

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Residual oil from babassu (Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B from Candida antarctica (Novozym® 435) and ethanol. For the first time in the literature, residual babassu oil and Novozym® 435 are being investigated to obtain biodiesel. In this communication, response surface methodology (RSM) and a central composite design (CCD) were used to optimize the esterification and study the effects of four factors (molar ratio (1:1–1:16, free fatty acids (FFAs) /alcohol), temperature (30–50 °C), biocatalyst content (0.05–0.15 g) and reaction time (2–6 h)) in the conversion into fatty acid ethyl esters. Under optimized conditions (1:18 molar ratio (FFAs/alcohol), 0.14 g of Novozym® 435, 48 °C and 4 h), the conversion into ethyl esters was 96.8%. It was found that after 10 consecutive cycles of esterification under optimal conditions, Novozym® 435 showed a maximum loss of activity of 5.8%, suggesting a very small change in the support/enzyme ratio proved by Fourier Transform Infrared (FTIR) spectroscopy and insignificant changes in the surface of Novozym® 435 proved by scanning electron microscopy (SEM) after the 10 consecutive cycles of esterification.

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“…In one study undertaken in a packed bed, Krause and Fieg [12] studied the hydrolysis of methyl octanoate. The catalyst was Novozym 435, namely lipase B of Candida antarctica immobilized on poly-(methyl methacrylate) beads [35]. The reaction medium supplied to the packed bed was an ester in water emulsion; in other words, it contained organic and aqueous phases.…”

Section: Discussionmentioning

confidence: 99%

Analysis of multiphasic behavior during the ethyl esterification of fatty acids catalyzed by a fermented solid with lipolytic activity in a packed-bed bioreactor in a closed-loop batch system

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Corazza

et al. 2015

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Investigation of the stability of Novozym® 435 in the production of biodiesel

Cited by 30 publications

References 26 publications

Synthesis of Ibuprofen Monoglyceride in Solventless Medium with Novozym®435: Kinetic Analysis

Synthesis of Ibuprofen Monoglyceride in Solventless Medium with Novozym®435: Kinetic Analysis

Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM

Analysis of multiphasic behavior during the ethyl esterification of fatty acids catalyzed by a fermented solid with lipolytic activity in a packed-bed bioreactor in a closed-loop batch system

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