Investigation of the Species Origin of the St. Jude Porcine Lung Epithelial Cell Line (SJPL) Made Available to Researchers

Silversides, David W.; Music, Nedzad; Jacques, Mario; Gagnon, Carl A.; Webby, Richard J.

doi:10.1128/jvi.00042-10

Cited by 23 publications

(17 citation statements)

References 7 publications

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“…Noteworthy, during the course of this study, the SJPL cell line was found to be of monkey origin based on karyotype and genetic analyses [42]. Nevertheless, the results of the present study show that SJPL cells are: 1) permissive to PRRSV replication and 2) phenotypically different from MARC-145 cells.…”

Section: Introductionmentioning

confidence: 60%

“…At the beginning of this project, the SJPL cells were reported to be epithelial cells of the respiratory tract of swine [41,54]. During the course of this study however, these cells were found to be of monkey origin [42], suggesting that they could be phenotypically similar to MARC-145 cells, another monkey cell line. In addition, the SJPL cells have been known to be permissive to a variety of sub-types of influenza virus from human, swine, avian and horse origins [41].…”

Section: Discussionmentioning

confidence: 99%

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Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

Provost

Jia

Music

et al. 2012

Virol J

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BackgroundAirborne transmitted pathogens, such as porcine reproductive and respiratory syndrome virus (PRRSV), need to interact with host cells of the respiratory tract in order to be able to enter and disseminate in the host organism. Pulmonary alveolar macrophages (PAM) and MA104 derived monkey kidney MARC-145 cells are known to be permissive to PRRSV infection and replication and are the most studied cells in the literature. More recently, new cell lines developed to study PRRSV have been genetically modified to make them permissive to the virus. The SJPL cell line origin was initially reported to be epithelial cells of the respiratory tract of swine. Thus, the goal of this study was to determine if SJPL cells could support PRRSV infection and replication in vitro.ResultsThe SJPL cell growth was significantly slower than MARC-145 cell growth. The SJPL cells were found to express the CD151 protein but not the CD163 and neither the sialoadhesin PRRSV receptors. During the course of the present study, the SJPL cells have been reported to be of monkey origin. Nevertheless, SJPL cells were found to be permissive to PRRSV infection and replication even if the development of the cytopathic effect was delayed compared to PRRSV-infected MARC-145 cells. Following PRRSV replication, the amount of infectious viral particles produced in SJPL and MARC-145 infected cells was similar. The SJPL cells allowed the replication of several PRRSV North American strains and were almost efficient as MARC-145 cells for virus isolation. Interestingly, PRRSV is 8 to 16 times more sensitive to IFNα antiviral effect in SJPL cell in comparison to that in MARC-145 cells. PRRSV induced an increase in IFNβ mRNA and no up regulation of IFNα mRNA in both infected cell types. In addition, PRRSV induced an up regulation of IFNγ and TNF-α mRNAs only in infected MARC-145 cells.ConclusionsIn conclusion, the SJPL cells are permissive to PRRSV. In addition, they are phenotypically different from MARC-145 cells and are an additional tool that could be used to study PRRSV pathogenesis mechanisms in vitro.

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Section: Introductionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

Provost

Jia

Music

et al. 2012

Virol J

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“…Quantitative real-time PCR (qRT-PCR) assays were performed to compare the expression of type IV fimbriae cluster genes apfA, apfB, and apfC of A. pleuropneumoniae in contact or without contact with St. Jude porcine lung (SJPL) cells (kindly donated by Robert G. Webster, St. Jude Children's Research Hospital). This cell line has been used as a model of porcine lung epidermal cells (19,20), although recent findings indicated that the SJPL cell line is not of porcine origin (21). The protocol used for A. pleuropneumoniae cultured alone or cocultured with SJPL cells was as previously described (22).…”

Section: Methodsmentioning

confidence: 99%

Adhesion Protein ApfA of Actinobacillus pleuropneumoniae Is Required for Pathogenesis and Is a Potential Target for Vaccine Development

Zhou

Chen

et al. 2013

Clin Vaccine Immunol

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Actinobacillus pleuropneumoniae is the etiologic agent of porcine pleuropneumonia, which causes serious economic losses in the pig farming industry worldwide. Due to a lack of knowledge of its virulence factors and a lack of effective vaccines able to confer cross-serotype protection, it is difficult to place this disease under control. By analyzing its genome sequences, we found that type IV fimbrial subunit protein ApfA is highly conserved among different serotypes of A. pleuropneumoniae. Our study shows that ApfA is an adhesin since its expression was greatly upregulated (135-fold) upon contact with host cells, while its deletion mutant attenuated its capability of adhesion. The inactivation of apfA dramatically reduced the ability of A. pleuropneumoniae to colonize mouse lung, suggesting that apfA is a virulence factor. Purified recombinant ApfA elicited an elevated humoral immune response and conferred robust protection against challenges with A. pleuropneumoniae serovar 1 strain 4074 and serovar 7 strain WF83 in mice. Importantly, the anti-ApfA serum conferred significant protection against both serovar 1 and serovar 7 in mice. These studies indicate that ApfA promotes virulence through attachment to host cells, and its immunogenicity renders it a promising novel subunit vaccine candidate against infection with A. pleuropneumoniae.

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“…The HRT-18 cell line, a human colorectal adenocarcinoma cell line, was grown in RPMI medium supplemented with 10% fetal bovine serum (Gibco), 1% penicillin-streptomycin (100ϫ; Gibco), and 1% amphotericin B (Fungizone; 250 g/ml; Gibco). The HEp-2 cell line, derived from human larynx carcinoma, and the SJPL cell line, of monkey origin (18), were grown in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (Gibco), 1% penicillin-streptomycin (100ϫ; Gibco), and 1% amphotericin B (250 g/ml; Gibco). Cells were grown in T175 flasks (Sarstedt, Nümbrecht, Germany) at 37°C under an atmosphere enriched with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

High-Throughput Microfluidic Method To Study Biofilm Formation and Host-Pathogen Interactions in Pathogenic Escherichia coli

Tremblay

Vogeleer

Jacques

et al. 2015

Appl Environ Microbiol

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Biofilm formation and host-pathogen interactions are frequently studied using multiwell plates; however, these closed systems lack shear force, which is present at several sites in the host, such as the intestinal and urinary tracts. Recently, microfluidic systems that incorporate shear force and very small volumes have been developed to provide cell biology models that resemble in vivo conditions. Therefore, the objective of this study was to determine if the BioFlux 200 microfluidic system could be used to study host-pathogen interactions and biofilm formation by pathogenic Escherichia coli. Strains of various pathotypes were selected to establish the growth conditions for the formation of biofilms in the BioFlux 200 system on abiotic (glass) or biotic (eukaryotic-cell) surfaces. Biofilm formation on glass was observed for the majority of strains when they were grown in M9 medium at 30°C but not in RPMI medium at 37°C. In contrast, HRT-18 cell monolayers enhanced binding and, in most cases, biofilm formation by pathogenic E. coli in RPMI medium at 37°C. As a proof of principle, the biofilm-forming ability of a diffusely adherent E. coli mutant strain lacking AIDA-I, a known mediator of attachment, was assessed in our models. In contrast to the parental strain, which formed a strong biofilm, the mutant formed a thin biofilm on glass or isolated clusters on HRT-18 monolayers. In conclusion, we describe a microfluidic method for high-throughput screening that could be used to identify novel factors involved in E. coli biofilm formation and host-pathogen interactions under shear force. Biofilms are defined as bacterial communities encased in a selfproduced polymeric matrix that is attached to a surface (1). The ability to form a biofilm is virtually a universal trait of bacteria and other microorganisms. Growth as a biofilm offers protection against hostile environments, the immune response, and bactericidal concentrations of antibiotics or disinfectants (1). Biofilms have frequently been studied using the 96-microtiter plate model because it is a platform that requires small volumes and is suited for high-throughput screening (1). The major flaws of the microtiter model are that it is a closed system and does not incorporate shear force. It is generally accepted that biofilms will develop in the presence of shear force in the environment (1). The MBEC assay, formerly known as the Calgary Biofilm Device, was developed to incorporate shear force into high-throughput screens (2); however, this model is a closed system. In a closed system, dispersing signals and metabolic waste accumulate, and nutrients become depleted. These events are not always favorable for biofilm studies and may lead to the rapid dispersal of the biofilm before it is quantified.Open systems, which have both a continuous flow of fresh medium and shear force, have been developed and are frequently used in the laboratory (1). These include the drip-flow reactor, flow cells, perfused biofilm fermenters, the CDC biofilm reactor, the rotating-disc re...

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Investigation of the Species Origin of the St. Jude Porcine Lung Epithelial Cell Line (SJPL) Made Available to Researchers

Cited by 23 publications

References 7 publications

Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

Identification of a new cell line permissive to porcine reproductive and respiratory syndrome virus infection and replication which is phenotypically distinct from MARC-145 cell line

Adhesion Protein ApfA of Actinobacillus pleuropneumoniae Is Required for Pathogenesis and Is a Potential Target for Vaccine Development

High-Throughput Microfluidic Method To Study Biofilm Formation and Host-Pathogen Interactions in Pathogenic Escherichia coli

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