Investigation of the soil nematode community composition in a monoculture Robusta coffee plantation in Dak Lak, Vietnam

Hoang, Ha; Pham, Huong Mai; Chu, Phuong Thi Minh; Nguyen, Trang Hong; Tran, Linh Huyen; Trinh, Phap Quang; Boer, Tjalf de; Brouwer, Abraham; Nguyen, Duong Thi Anh; Chu, Ha Hoang

doi:10.1016/j.gecco.2021.e01932

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…However, we believe the presence of other helminth species, namely Trichostrongylus and Meloidogyne eggs, which have a morphology similar to hookworm, albeit larger in size, and are easily confused with hookworm eggs [41][42][43], is the main contributor to false positives by microscopy. Meloidogyne eggs have been documented in human stool samples in this province of Vietnam [44,45], and Trichostrongylus infection in humans has been reported in neighboring Thailand and Laos [46][47][48]. Further work is underway to identify these species more definitively using qPCR techniques.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Assessing the efficacy of albendazole against hookworm in Vietnam using quantitative PCR and sodium nitrate flotation

Dyer

Clarke²,

Nguyen³

et al. 2022

PLoS Negl Trop Dis

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Preventive chemotherapy (PC), consisting of the regular distribution of anthelmintics to populations or groups of populations at risk, is the primary tool used to control soil-transmitted helminth (STH) infections. This strategy, whilst cost-effective, raises the concern of potential emergence of drug resistance. The efficacy of anthelmintics against STH infections is measured using cure rate (CR) and egg reduction rate (ERR), using microscopy-based techniques such as the Kato-Katz thick smear. However, Kato-Katz has low sensitivity, especially for low-intensity infections, and requires fresh samples that need to be processed quickly. Realtime quantitative PCR (qPCR), which is more sensitive, is emerging as a “gold standard” for STH diagnostics given its higher sensitivity (important in low prevalence settings) and ability to differentiate hookworm species, while sodium nitrate flotation (SNF) may provide a low-cost more sensitive and practical alternative to Kato-Katz in the field. In this study, we examined the efficacy of a locally manufactured brand of albendazole 400 mg (“Alzental”) against hookworm in Đắk Lắk province, Vietnam, using both qPCR and SNF. For qPCR, formulae to convert qPCR cycle threshold (Ct) values into eggs per gram of faeces (EPG) were utilised to determine efficacy calculations, and these values directly compared with efficacy values generated using SNF. Factors associated with CR and ERR were examined, and Alzental tablet quality was assessed by comparing with an Australian TGA-approved equivalent “Eskazole” tablet. We observed a CR and ERR of 64.9% and 87.5% respectively using qPCR, and 68.4% and 67.6% respectively using SNF. The tablet composition of Alzental was comparable to Eskazole in terms of active albendazole drug concentration with no evidence of impurities. This study demonstrates that the efficacy of Alzental against hookworm is within the range of previously reported studies for albendazole 400 mg. The study also demonstrates the value of qPCR and SNF as alternatives to standard Kato-Katz methodology for assessment of anthelmintic efficacy.

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Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Assessing the efficacy of albendazole against hookworm in Vietnam using quantitative PCR and sodium nitrate flotation

Dyer

Clarke²,

Nguyen³

et al. 2022

PLoS Negl Trop Dis

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Preparation of sulfur nanoparticles in chitosan-copper complex and investigation of its nematicidal activity against Pratylenchus pratensis in vitro

Du Bui,

Nguyen,

Tran

et al. 2024

Pure and Applied Chemistry

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Sulfur nanoparticles (SNPs) in chitosan-copper (CS-Cu2+) complex solution were prepared by hydrolysis of sodium thiosulfate (Na2S2O3) in an acidic medium of CS-Cu2+ complex. The size of SNPs was inversely proportional to the Cu2+/−NH2 molar ratio, decreasing from 35 to 22 nm corresponding to the Cu2+/−NH2 molar ratio increasing from 0/1 to 1/1. The SNPs/CS-Cu2+ complex was characterized by Ultraviolet-Visible spectroscopy (UV–Vis), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD) spectroscopy. The nematicidal activity against Pratylenchus pratensis in vitro was investigated by treating the CS-Cu2+ complex and SNPs/CS-Cu2+ complex with Cu2+/−NH2 molar ratio of 0.5/1. Results showed that the nematicidal activity of the SNPs/CS-Cu2+ complex was higher than that of the CS-Cu2+ complex, particularly the 50 % lethal dose (LC50) after 48 h of treatment was 77 and 89 mg/L, respectively. The results demonstrated that the prepared SNPs/CS-Cu2+ complex can be used as a nematicide for plants.

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Investigation of the soil nematode community composition in a monoculture Robusta coffee plantation in Dak Lak, Vietnam

Cited by 2 publications

References 12 publications

Assessing the efficacy of albendazole against hookworm in Vietnam using quantitative PCR and sodium nitrate flotation

Assessing the efficacy of albendazole against hookworm in Vietnam using quantitative PCR and sodium nitrate flotation

Preparation of sulfur nanoparticles in chitosan-copper complex and investigation of its nematicidal activity against Pratylenchus pratensis in vitro

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