Investigation of the Reuse of Immobilized Lipases in Biodiesel Synthesis: Influence of Different Solvents in Lipase Activity

Aguieiras, Érika C.G.; Ribeiro, Douglas; Couteiro, Pedro P.; Bastos, C. M. B.; Queiroz, Danielle Simas de; Parreira, Juliana M.; Langone, Marta A. P.

doi:10.1007/s12010-016-2008-9

Cited by 41 publications

(31 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hexane was reported to result in the lowest amount of glycerol removal and lower enzyme decay activity due to its hydrophobic properties. However the use of polar solvent such as ethanol can lead to the change of support morphology by dissolving the lipase supports 20 . Furthermore, the combined effects of desorption or leakage of enzyme from the support as well as mechanical loss during repeated filtration, washing, and drying activities could reduce the yield of palm esters 16, 20 23 .…”

Section: Enzyme Reusability In a Solvent-free Reaction Systemmentioning

confidence: 99%

Lipase-catalyzed Production and Purification of Palm Esters Using Stirred Tank Reactors (STR)

et al. 2019

View full text Add to dashboard Cite

Palm-based specialty oleochemicals, particularly palmbased wax esters are one of the palm oil product derivatives that may have a very promising future. Wax esters, consisting of long chain fatty acids and long chain fatty alcohols exhibit excellent wetting behavior at the interface and non-irritating properties on human skin 2 5. However, the natural supply of wax esters such as jojoba oil and sperm whale oil are often expensive and scarce due to weather limitations and the global ban on whale hunting, leading to a growing interest in the production of synthetic wax esters, which possess similar characteristics as the natural wax esters but at a lower cost. Research on these high value palm-based wax esters has developed steadily over the past decade. Few studies have been made on its up-scale production despite its wide range application especially in cosmetics, food, pharmaceutical and chemical industries. Conventionally, the produc-Abstract: Lipase-catalyzed production of palm esters was performed via alcoholysis of palm oil and oleyl alcohol in solvent and solvent-free systems using a 2 L stirred tank reactor (STR). Two immobilized lipases were tested and Lipozyme RM IM exhibited superior performance in both reaction systems. Reusability studies of the enzymes in a solvent-free system also demonstrated the high stability of Lipozyme RM IM as shown by its ability to yield more than 70% palm esters with up to 19 cycles of reusing the same enzymes. Modification of the enzyme washing process improved the stability of Lipozyme TL IM in a solvent system as demonstrated by maintaining 65% yield after 5 times of repeated enzyme use. The scale up process for both lipases was conducted in the presence of solvents by using the impeller tip speed approach. Lipozyme RM IM-catalyzed reaction in a 15 L STR produced 85.7% yield and there was a significant drop to 60.7% in the 300 L STR, whereas Lipozyme TL IM had a lower yield (65%) when the reaction volume was increased to 15 L. The low yields could be due to the accumulation of enzymes at the bottom of the vessel. Purification of palm esters via solvent-solvent extraction revealed that more than 90% of oleyl alcohol was extracted after the third extraction cycle at 150 rpm impeller speed with reduced palm esters: ethanol ratio (v/v) from 1:4 to 1:3.

show abstract

Section: Enzyme Reusability In a Solvent-free Reaction Systemmentioning

confidence: 99%

Lipase-catalyzed Production and Purification of Palm Esters Using Stirred Tank Reactors (STR)

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The recovered biocatalyst was then washed with 20 mL of Butanol (log P = 0.88). Differences in solvent polarity have been used to explain their effect on the catalytic activity and specificity of reused CAL B [19]. Highest enzyme activities and stabilities are generally observed in solvents with high log P values.…”

Section: Enzyme Reusementioning

confidence: 99%

“…Using renewables as raw materials makes the conversion processes fully ecologically friendly and the lipase-catalyzed synthesis also has the advantage that the product may be accepted as a natural product [10]. Furthermore, stability and activity of CAL B were studied in repetitive cycle reuses [11]. Butanol was used to wash the recycled lipase.…”

Section: Introductionmentioning

confidence: 99%

Preparation and evaluation of xylose palmitate ester using Candida antarctica lipase B as a reusable biocatalyst

2018

View full text Add to dashboard Cite

T HE activity and stability of lipase B from Candida antarctica were investigated in direct enzymatic esterification of D-xylose with palmitic acid using ethylmethylketone as reaction media. The xylose palmitate was obtained under the optimized conditions, 1:1 molar ratio of xylose: palmitic acid, at 250 rpm and 60°C. Effect of the use of Butanol to wash the immobilized lipase before the enzyme reuse was evaluated. The lipase could be successfully reused four times without loss of activity. Various physicochemical characteristics of 1-O-hexadecanoyl-D-xylopyranose were also studied.

show abstract

“…The use of very hydrophobic supports or ultrasounds may reduce these adverse effects [41][42][43][44][45][46][47][48]. On the other hand, biocatalyst loading in the reactor defines the kinetics aspects of the reaction, such as reaction rate and the occurrence of inhibition/inactivation, with a substantial impact on reaction time, productiveness, and process costs [42,[49][50][51][52]. As immobilized lipases are still expensive incomes, the definition of an optimized biocatalyst loading is a critical parameter for any applied biocatalytic process [44,[49][50][51][52].…”

Section: Introductionmentioning

confidence: 99%

Simplified Method to Optimize Enzymatic Esters Syntheses in Solvent-Free Systems: Validation Using Literature and Experimental Data

et al. 2021

View full text Add to dashboard Cite

The adoption of biocatalysis in solvent-free systems is an alternative to establish a greener esters production. An interesting correlation between the acid:alcohol molar ratio and biocatalyst (immobilized lipase) loading in the optimization of ester syntheses in solvent-free systems had been observed and explored. A simple mathematical tool named Substrate-Enzyme Relation (SER) has been developed, indicating a range of reaction conditions that resulted in high conversions. Here, SER utility has been validated using data from the literature and experimental assays, totalizing 39 different examples of solvent-free enzymatic esterifications. We found a good correlation between the SER trends and reaction conditions that promoted high conversions on the syntheses of short, mid, or long-chain esters. Moreover, the predictions obtained with SER are coherent with thermodynamic and kinetics aspects of enzymatic esterification in solvent-free systems. SER is an easy-to-handle tool to predict the reaction behavior, allowing obtaining optimum reaction conditions with a reduced number of experiments, including the adoption of reduced biocatalysts loadings.

show abstract

Investigation of the Reuse of Immobilized Lipases in Biodiesel Synthesis: Influence of Different Solvents in Lipase Activity

Cited by 41 publications

References 30 publications

Lipase-catalyzed Production and Purification of Palm Esters Using Stirred Tank Reactors (STR)

Lipase-catalyzed Production and Purification of Palm Esters Using Stirred Tank Reactors (STR)

Preparation and evaluation of xylose palmitate ester using Candida antarctica lipase B as a reusable biocatalyst

Simplified Method to Optimize Enzymatic Esters Syntheses in Solvent-Free Systems: Validation Using Literature and Experimental Data

Contact Info

Product

Resources

About