Investigation of The Relationship Between CARDS TX and The HMGB1-TLRs-MyD88 Signaling Pathway in MPP

Abstract

<jats:title>Abstract</jats:title> <jats:p><jats:bold>Background:</jats:bold> This study aimed to investigate the relationship between community-acquired respiratory distress syndrome toxin (CARDS TX) and HMGB1-TLRs-MyD88 in <jats:italic>Mycoplasma pneumoniae</jats:italic> pneumonia (MPP) and to examine the immune pathogenesis of <jats:italic>Mycoplasma pneumoniae</jats:italic> infection. <jats:bold>Methods:</jats:bold> Children who were diagnosed with MPP and examined by bronchoscopy were included in the MPP group. Additionally, children who underwent bronchoscopy because of bronchial foreign bodies in the same period were included in the control group. Gene expression of CARDS TX, high-mobility group box protein 1 (HMGB1), Toll-like receptor 2 (TLR2), Toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), and cluster of differentiation 14 (CD14) in bronchoalveolar lavage fluid (BALF) were detected using real-time reverse transcription-polymerase chain reaction. Correlations between CARDS TX and HMGB1-TLRs-MyD88 were analyzed. <jats:bold>Results:</jats:bold> CARDS TX, HMGB1, TLR2, MyD88, and CD14 mRNA expression in BALF in the MPP group was significantly higher than that in the control group (all <jats:italic>P</jats:italic>&lt; 0.05). CARDS TX mRNA expression was positively correlated with HMGB1, TLR2, MyD88, and CD14 mRNA expression (all <jats:italic>P</jats:italic>&lt; 0.05). Furthermore, HMGB1 mRNA expression was positively correlated with TLR2, MyD88, and CD14 mRNA expression (all <jats:italic>P</jats:italic>&lt; 0.05).<jats:bold> </jats:bold><jats:bold>Conclusions: </jats:bold>CARDS TX may participate in the immune pathogenesis of MPP through the HMGB1-TLRs/CD14-MyD88 pathway.</jats:p>