Investigation of the physiological properties and synthesis of PUFAs from Thraustochytrids and its electrophoretic karyotypes

Anbu, Periasamy; Kim, Donguk; Jeh, Eun-Jin; Jeong, Young‐Su; Hur, Byung‐Ki

doi:10.1007/bf02931091

Cited by 13 publications

(5 citation statements)

References 35 publications

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“…On the other hand, using YE at the high concentration decreased in lipid content in microalgal cells (14.36 ± 1.29 (at 1% YE) and 15.21 ± 0.18 (at 1.5% YE). The obtained results were completely consistent with the report of Anbu et al (2007Anbu et al ( ). et al (2018 showed that high YE level stimulated to cell growth but inhibition to lipid accumulation.…”

Section: Effect Of Ye Concentrationsupporting

confidence: 93%

Optimization of cultivation conditions of the heterotrophic marine microalga \(\textit{Thraustochytrium aureum}\) BT6 oriented to exploit bioactive compounds

Thom

Hien

Thu

et al. 2021

AJB

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Thraustochytrium is a heterotrophic marine microalgae genus belonging to the Labyrinthula class, Thraustochytriaceae (Thraustochytrids) family. Recently, studied results have showed that some species/strains of Thraustochytrium genus are potential candidates to produce biomass rich in lipid containing high polyunsaturated fatty acid (PUFAs), especially docosahexaenoic (DHA C22: 6 ω-3), carotenoid, extracellular polysaccharides (EPSs) and enzymes which play a crucial role in human health. Therefore, throughout many decades, numerous studies have been conducted in exploiting bioactive compounds, especially PUFAs. Biomass and PUFAs yield of some strains Thraustochytrium spp. depend on environmental conditions. In this paper, the results on the effects of different culture conditions on the growth of Thraustochytrium aureum BT6 (isolated from coastal regions of Binh Thuan in 2010) in 500 mL erlenmeyer flask and 30 L fermentors is presented. Suitable conditions for the growth of strain BT6 were: Bajpai medium, 2% glucose, 0.5% yeast extract, temperature of 20−28 oC, salt concentration 0.5%; and initial algal cell concentration of 2 g/L. The dry biomass, lipid and DHA contents of strain BT6 in 500 mL erlenmeyer flasks and 30 L fermentor reached the highest levels of 8.56 ± 0.12 and 6.92 ± 0.27 g/L, 18.36 ± 1.15 and 5.50 ± 0.133% DCW, 2.00 ± 0.05 and 0.14 ± 0.01% DCW, respectively, after 4 days of cultivation. Carotenoid content in flask and 30L fermentor achieved 44.45 ± 1.4 and 28.84 ± 1.12 mg/kg DCW, respectively after 4 days of cultivation. Obtained results have indicated that the algae biomass met the requirements in orientation to exploit valuable bioactive compounds.

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Section: Effect Of Ye Concentrationsupporting

confidence: 93%

Optimization of cultivation conditions of the heterotrophic marine microalga \(\textit{Thraustochytrium aureum}\) BT6 oriented to exploit bioactive compounds

Thom

Hien

Thu

et al. 2021

AJB

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“…A single thallus in the process of becoming a sporangium could have dozens of copies of the genome but be counted microscopically as a single cell. Because the propor- (2). Further work will be required to determine whether the apparently high DNA content of QPX reflects its life history or other factors.…”

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confidence: 99%

Quantitative Real-Time PCR Assay for QPX (Thraustochytriidae), a Parasite of the Hard Clam ( Mercenaria mercenaria )

Liu

Allam

Collier

2009

Appl Environ Microbiol

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We developed a real-time quantitative PCR (qPCR) assay targeting the rRNA internal transcribed spacer region of the hard clam pathogen QPX. The qPCR assay was more sensitive than was histology in detecting clams with light QPX infections. QPX was detected in 4 of 43 sediment samples but in none of 40 seawater samples.The thraustochytrid called QPX (for quahog parasite unknown) has caused high mortalities in hatchery-reared and wild hard clams (Mercenaria mercenaria, also known as quahogs) from Prince Edward Island (Canada) to Virginia (United States) since the late 1950s (17,22,25,29). In the summer of 2002, QPX infections appeared in the previously healthy Raritan Bay (off the coast of Staten Island in New York) M. mercenaria population, causing significant clam mortality and closure of the fishery (6). Management of hard clam populations affected by QPX disease is hampered by an incomplete understanding of factors controlling the occurrence and severity of QPX infections. Environmental factors, such as salinity and temperature, appear to be important (22), as do clam population density and the planting of seed from nonlocal sources (7). More quantitative information about the occurrence and progression of QPX disease in relation to these and other variables would support better prediction of, and response to, QPX outbreaks. QPX is thought to be an opportunistic pathogen (4, 7, 11), capable of growing outside its host. However, there is very little known about substrates that might support QPX organisms outside of hard clams (4). The abilities to detect and enumerate QPX cells in potential reservoirs would allow the dynamics of the QPX organism in the environment to be related to the occurrence of QPX disease, offering new insight into fundamental questions about the natural transmission mechanisms of the infection.The 18S ribosomal DNA (rDNA) primer pair QPX-F and QPX-R2 can be used in a standard PCR assay to detect the presence of QPX DNA in clam tissue samples (26). Unfortunately, the products are too long (ϳ650 bp), and often include too much primer dimer, for use in a SYBR green real-time quantitative PCR (qPCR) assay. The low sequence variability in rRNA genes made it difficult to design other primers specific for QPX 18S rDNA. Instead, we used our previously reported rRNA internal transcribed spacer (ITS) region (including ITS1, the 5.8S rRNA gene, and ITS2) sequences for QPX isolates from Massachusetts and New York (20) to develop a qPCR assay targeting the more variable ITS region (1).Development of QPX-specific real-time qPCR assay. The ITS regions of the thraustochytrids Schizochytrium aggregatum (ATCC 28209), Schizochytrium limacinum (ATCC MYA-1381), and Thraustochytrium aureum (ATCC 34304) (acquired from the American Type Culture Collection, Manassas, VA, and maintained in medium 790 Byϩ at 23°C) were PCR amplified with universal 18S and 28S rDNA primers (18S-RR and 28S46Rev) (Table 1), cloned and sequenced as described previously (20), and submitted to GenBank (http://www.ncbi.nlm .nih.gov) under ...

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“…Recently, T. aureum has received considerable attention due to its ability to produce several PUFAs [31][32][33]. The present study focuses on identification of the elongase gene, which is involved in the elongation of C20 PUFAs.…”

Section: Resultsmentioning

confidence: 99%

Identification of Δ9-elongation activity from Thraustochytrium aureum by heterologous expression in Pichia pastoris

Lee

Anbu

Kim

et al. 2008

Biotechnol Bioproc E

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Investigation of the physiological properties and synthesis of PUFAs from Thraustochytrids and its electrophoretic karyotypes

Cited by 13 publications

References 35 publications

Optimization of cultivation conditions of the heterotrophic marine microalga \(\textit{Thraustochytrium aureum}\) BT6 oriented to exploit bioactive compounds

Optimization of cultivation conditions of the heterotrophic marine microalga \(\textit{Thraustochytrium aureum}\) BT6 oriented to exploit bioactive compounds

Quantitative Real-Time PCR Assay for QPX (Thraustochytriidae), a Parasite of the Hard Clam ( Mercenaria mercenaria )

Identification of Δ9-elongation activity from Thraustochytrium aureum by heterologous expression in Pichia pastoris

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