Investigation of the physiochemical properties, cryoprotective activity and possible action mechanisms of sericin peptides derived from membrane separation

Intracellular Expression of Antifreeze Peptides in Food Grade Lactococcus lactis and Evaluation of Their Cryoprotective Activity

“…Cells were suspended and diluted with PBS buffer (0.02 mol/L, pH 7.4) to an approximate concentration of 10 7 CFU/mL and further used for flow cytometric analysis according to the method descripted by Li et al. ().…”

“…& Jian, 2012). Moreover, antifreeze peptides have been found to be a novel and effective cryoprotectant for the lactic acid bacteria when they are exposed to freezing temperatures (Li et al, 2017). Therefore, in this work, we aimed to construct a recombinant L. lactis expressing antifreeze peptides intracellularly by using a food grade NICE (NIsin-Controlled Expression) expression system and L. lactis subsp.…”

“…In order to distinguish the physiological state of cells after freezing, proliferating phase L. lactis cells with or without freezing from the strain SF-P 1 , SF-P 2 , and NZ3900 were prepared, respectively. Cells were suspended and diluted with PBS buffer (0.02 mol/L, pH 7.4) to an approximate concentration of 10 7 CFU/mL and further used for flow cytometric analysis according to the method descripted by Li et al (2017).…”

“…It has been reported that there are two kinds of protective agents: small molecule (such as amino acids, sugar alcohols, fatty acids, oligosaccharides, and surfactants) and macromolecular (such as polysaccharides, proteins, and peptides) (Wang, Chen, Wu, & Wang, ; Zhang, Yin, Wei, Du, & Jian, ). Moreover, antifreeze peptides have been found to be a novel and effective cryoprotectant for the lactic acid bacteria when they are exposed to freezing temperatures (Li et al., ).…”

Cryoprotective Effects of Protein Hydrolysates Prepared from By-Products of Silver Carp (Hypophthalmichthys Molitrix) on Freeze-Thawed Surimi

“…PH and AH were also rich in Pro residues (about 13%) which also contributed to ice affinity [23]. Sericin hydrolysates (molecular weight of less than 3 kD) with cryoprotective activity were also rich in the amino acids Ser, Asp, Gly, Thr and Glu [24]. In ice-binding proteins from arctic yeast, aligned Thr/Ser/Ala residues were found to be critical for binding of ice [25].…”