Investigation of the multifunctional gene AOP3 expands the regulatory network fine-tuning glucosinolate production in Arabidopsis

Jensen, Lea Møller; Kliebenstein, Daniel J.; Burow, Meike

doi:10.3389/fpls.2015.00762

Cited by 11 publications

(13 citation statements)

References 43 publications

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“…Candidate gene BnaC09g00410D is homologous to AOP3 , a key gene in increasing leaf glucosinolate accumulation in Arabidopsis (Jensen et al ). However, we detected a negative regulatory effect of BnaC09g00410D on seed glucosinolate accumulation in B. napus .…”

Section: Discussionmentioning

confidence: 99%

Genome‐wide identification of loci affecting seed glucosinolate contents in Brassica napus L.

Wei

Cui

Mei

et al. 2018

JIPB

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Glucosinolates are amino acid-derived secondary metabolites that act as chemical defense agents against pests. However, the presence of high levels of glucosinolates severely diminishes the nutritional value of seed meals made from rapeseed (Brassica napus L.). To identify the loci affecting seed glucosinolate content (SGC), we conducted genome-wide resequencing in a population of 307 diverse B. napus accessions from the three B. napus ecotype groups, namely, spring, winter, and semi-winter. These resequencing data were used for a genome-wide association study (GWAS) to identify the loci affecting SGC. In the three ecotype groups, four common and four ecotype-specific haplotype blocks (HBs) were significantly associated with SGC. To identify candidate genes controlling SGC, transcriptome analysis was carried out in 36 accessions showing extreme SGC values. Analyses of haplotypes, genomic variation, and candidate gene expression pointed to five and three candidate genes in the common and spring group-specific HBs, respectively. Our expression analyses demonstrated that additive effects of the three candidate genes in the spring group-specific HB play important roles in the SGC of B. napus.

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Section: Discussionmentioning

confidence: 99%

Genome‐wide identification of loci affecting seed glucosinolate contents in Brassica napus L.

Wei

Cui

Mei

et al. 2018

JIPB

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“…The resulting extract was diluted 5.0 fold with water and the diluted samples were directly analyzed by LC-MS/qTOF for identification of native GLSs. For quantification, GLSs were analyzed as desulfo-GLSs (dsGLSs) after enzymatic on-column desulfation as previously described (Jensen et al, 2015;Crocoll et al, 2016a). Allyl GLS (K + salt, PhytoLab, Vestenbergsgreuth, Germany) was added as internal standard before desulfation with a final concentration of 1 µM.…”

Section: Plant Extraction and Gls Analysismentioning

confidence: 99%

“…GLSs were analyzed as dsGLSs after enzymatic desulfation as previously described (Jensen et al, 2015;Crocoll et al, 2016a) with modifications for separation of leucine and isoleucinederived dsGLSs. Briefly, chromatography was performed on an Advance UHPLC system (Bruker, Bremen, Germany).…”

Section: Gls Analysis By Desulfation and Lc-ms/ Triple Quadrupolementioning

confidence: 99%

“…Bruker MS Workstation software (Version 8.2.1, Bruker, Bremen, Germany) was used for data acquisition and processing. Allyl GLS was used as internal standard for quantification as previously described (Jensen et al, 2015). Authentic references of ds1ME, ds1MP, and ds2MP were obtained as previously reported (Olsen et al, 2016).…”

Section: Gls Analysis By Desulfation and Lc-ms/ Triple Quadrupolementioning

confidence: 99%

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Characterization of Arabidopsis CYP79C1 and CYP79C2 by Glucosinolate Pathway Engineering in Nicotiana benthamiana Shows Substrate Specificity Toward a Range of Aliphatic and Aromatic Amino Acids

et al. 2020

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Glucosinolates (GLSs) are amino acid-derived defense compounds characteristic of the Brassicales order. Cytochromes P450s of the CYP79 family are the entry point into the biosynthetic pathway of the GLS core structure and catalyze the conversion of amino acids to oximes. In Arabidopsis thaliana, CYP79A2, CYP79B2, CYP79B3, CYP79F1, and CYP79F2 have been functionally characterized and are responsible for the biosynthesis of phenylalanine-, tryptophan-, and methionine-derived GLSs, respectively. However, the substrate(s) for CYP79C1 and CYP79C2 were unknown. Here, we investigated the function of CYP79C1 and CYP79C2 by transiently co-expressing the genes together with three sets of remaining genes required for GLS biosynthesis in Nicotiana benthamiana. Co-expression of CYP79C2 with either the aliphatic or aromatic core structure pathways resulted in the production of primarily leucine-derived 2-methylpropyl GLS and phenylalanine-derived benzyl GLS, along with minor amounts of GLSs from isoleucine, tryptophan, and tyrosine. Co-expression of CYP79C1 displayed minor amounts of GLSs from valine, leucine, isoleucine, and phenylalanine with the aliphatic core structure pathway, and similar GLS profile (except the GLS from valine) with the aromatic core structure pathway. Additionally, we co-expressed CYP79C1 and CYP79C2 with the chain elongation and aliphatic core structure pathways. With the chain elongation pathway, CYP79C2 still mainly produced 2-methylpropyl GLS derived from leucine, accompanied by GLSs derived from isoleucine and from chain-elongated mono-and dihomoleucine, but not from phenylalanine. However, co-expression of CYP79C1 only resulted in GLSs derived from chain-elongated amino acid substrates, dihomoleucine and dihomomethionine, when the chain elongation pathway was present. This shows that

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“…If the same eQTL is found for several genes, a so‐called ‘hot‐spot’, this locus is likely to harbour a master regulator (Breitling et al ., ). Additionally, as the differences in gene expression underlie many phenotypic and physiological differences between genotypes, QTLs can be shared between genes and other quantitative traits (Fu et al ., ; van Zanten et al ., ; Burow et al ., ; Kerwin et al ., , ; Joosen et al ., ; He et al ., ; Jimenez‐Gomez, ; Jensen et al ., ,b). Moreover when gene expression is measured in multiple populations, plant stages, tissues or environments, the variation in these traits can be placed in a gene‐by‐environment context.…”

Section: Introductionmentioning

confidence: 99%

AraQTL – workbench and archive for systems genetics in Arabidopsis thaliana

et al. 2017

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SUMMARYGenetical genomics studies uncover genome-wide genetic interactions between genes and their transcriptional regulators. High-throughput measurement of gene expression in recombinant inbred line populations has enabled investigation of the genetic architecture of variation in gene expression. This has the potential to enrich our understanding of the molecular mechanisms affected by and underlying natural variation. Moreover, it contributes to the systems biology of natural variation, as a substantial number of experiments have resulted in a valuable amount of interconnectable phenotypic, molecular and genotypic data. A number of genetical genomics studies have been published for Arabidopsis thaliana, uncovering many expression quantitative trait loci (eQTLs). However, these complex data are not easily accessible to the plant research community, leaving most of the valuable genetic interactions unexplored as cross-analysis of these studies is a major effort. We address this problem with AraQTL (http://www.bioinformatics.nl/AraQTL/), an easily accessible workbench and database for comparative analysis and meta-analysis of all published Arabidopsis eQTL datasets. AraQTL provides a workbench for comparing, re-using and extending upon the results of these experiments. For example, one can easily screen a physical region for specific local eQTLs that could harbour candidate genes for phenotypic QTLs, or detect gene-by-environment interactions by comparing eQTLs under different conditions.

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Investigation of the multifunctional gene AOP3 expands the regulatory network fine-tuning glucosinolate production in Arabidopsis

Cited by 11 publications

References 43 publications

Genome‐wide identification of loci affecting seed glucosinolate contents in Brassica napus L.

Genome‐wide identification of loci affecting seed glucosinolate contents in Brassica napus L.

Characterization of Arabidopsis CYP79C1 and CYP79C2 by Glucosinolate Pathway Engineering in Nicotiana benthamiana Shows Substrate Specificity Toward a Range of Aliphatic and Aromatic Amino Acids

AraQTL – workbench and archive for systems genetics in Arabidopsis thaliana

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