Investigation of the molecular mechanisms of hepatic injury upon naphthalene exposure in zebrafish (Danio rerio)

Chen, Hongshan; Sheng, Lianxi; Gong, Zhiyuan; Ru, Shaoguo; Bian, Hongfeng

doi:10.1007/s10646-018-1943-3

Cited by 10 publications

(3 citation statements)

References 60 publications

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“…The hub genes selection identified key actors in the global temperature response within each module, often known as key regulators on biological pathways. Among the blue module, we identified several tRNA ligases but also the YTH domain-containing family protein 2 and the eukaryotic translation elongation factor 1 epsilon-1 that play a role in RNA protection following heat stress and DNA protection after damage, respectively (Lewis et al 2017; Chen et al 2018). Among the mediumorchid module we found cyclic AMP-dependent transcription factors (namely ATF-4 and -5) that are transcription factors associated with circadian rhythm regulation (Supplementary Figure S1) as well as the cryptochrome-1 coding gene, a core repressor of circadian rhythm (Hardie et al 2012).…”

Section: Resultsmentioning

confidence: 99%

Domestication and Temperature Modulate Gene Expression Signatures and Growth in the Australasian Snapper Chrysophrys auratus

Wellenreuther

Luyer

Cook

et al. 2019

G3 Genes|Genomes|Genetics

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Identifying genes and pathways involved in domestication is critical to understand how species change in response to human-induced selection pressures, such as increased temperatures. Given the profound influence of temperature on fish metabolism and organismal performance, a comparison of how temperature affects wild and domestic strains of snapper is an important question to address. We experimentally manipulated temperature conditions for F1-hatchery and wild Australasian snapper (Chrysophrys auratus) for 18 days to mimic seasonal extremes and measured differences in growth, white muscle RNA transcription and hematological parameters. Over 2.2 Gb paired-end reads were assembled de novo for a total set of 33,017 transcripts (N50 = 2,804). We found pronounced growth and gene expression differences between wild and domesticated individuals related to global developmental and immune pathways. Temperature-modulated growth responses were linked to major pathways affecting metabolism, cell regulation and signaling. This study is the first step toward gaining an understanding of the changes occurring in the early stages of domestication, and the mechanisms underlying thermal adaptation and associated growth in poikilothermic vertebrates. Our study further provides the first transcriptome resources for studying biological questions in this non-model fish species.

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Section: Resultsmentioning

confidence: 99%

Domestication and Temperature Modulate Gene Expression Signatures and Growth in the Australasian Snapper Chrysophrys auratus

Wellenreuther

Luyer

Cook

et al. 2019

G3 Genes|Genomes|Genetics

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“…Their capacity to provoke diverse toxic effects in aquatic organisms is well known. These effects range from cardiotoxicity and malformations at early developmental stages up to behavioural alterations and carcinogenesis in adults [27,54,78,129].…”

Section: Introductionmentioning

confidence: 99%

Multilevel responses of adult zebrafish to crude and chemically dispersed oil exposure

Esteban‐Sánchez

Johann

Alemany³

et al. 2021

Environ Sci Eur

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Background The application of chemical dispersants is a common remediation strategy when accidental oil spills occur in aquatic environments. Breaking down the oil slick into small droplets, dispersants facilitate the increase of particulate and dissolved oil compounds, enhancing the bioavailability of toxic oil constituents. The aim of the present work was to explore the effects of water accommodated fractions (WAF) of a naphthenic North Sea crude oil produced with and without the addition of the chemical dispersant FINASOL OSR 52 to adult zebrafish exposed for 3 and 21 d. Fish were exposed to environmentally relevant concentrations of 5% and 25% WAFOIL (1:200) and to 5% WAFOIL+D (dispersant–oil ratio 1:10) in a semi-static exposure setup. Results The chemically dispersed WAF presented a 20-fold increase of target polycyclic aromatic hydrocarbons (PAHs) in the water phase compared to the corresponding treatment without dispersant and was the only treatment resulting in markedly bioaccumulation of PAHs in carcass after 21 d compared to the control. Furthermore, only 5% WAFOIL+D caused fish mortality. In general, the undispersed oil treatments did not lead to significant effects compared to control, while the dispersed oil induced significant alterations at gene transcription and enzyme activity levels. Significant up-regulation of biotransformation and oxidative stress response genes (cyp1a, gstp1, sod1 and gpx1a) was recorded in the livers. For the same group, a significant increment in EROD activity was detected in liver along with significant increased GST and CAT activities in gills. The addition of the chemical dispersant also reduced brain AChE activity and showed a potential genotoxic effect as indicated by the increased frequency of micronuclei in erythrocytes after 21 d of exposure. Conclusions The results demonstrate that the addition of chemical dispersants accentuates the effect of toxic compounds present in oil as it increases PAH bioavailability resulting in diverse alterations on different levels of biological organization in zebrafish. Furthermore, the study emphasizes the importance to combine multilevel endpoints for a reliable risk assessment due to high variable biomarker responses. The present results of dispersant impact on oil toxicity can support decision making for oil spill response strategies.

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“…Benzopyrene also inhibits the growth of Phaeodactylum tricornutum and induces oxidative stress and lipid peroxidation in the extracellular cytoplasmic matrix (57). Treatment with naphthalene harms the liver, disrupts lipid metabolism, initiates inflammatory responses, and causes carcinogenic processes in zebrafish (11).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome changes ofTakifugu obscurusliver after acute exposure to the oxygenated-PAH 9,10-phenanthrenequione

Jiang

Yang

Fang

2020

Physiological Genomics

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Contamination with polycyclic aromatic hydrocarbons (PAHs) causes noticeable ecological problems in aquatic ecosystems. 9,10-phenanthrenequione (9,10-PQ) is an oxidized PAH and is highly toxic to aquatic animals. However, the effects of 9,10-PQ on the molecular metabolism of fish remain largely unknown. In this study, Takifugu obscurus juveniles were acutely exposed to 44.30 µg/L 9,10-PQ for three days. The transcriptome profile changes in their livers were compared between the 9,10-PQ treatment group and the control using T. rubripes as the reference genome. The results identified 22,414 genes in our transcriptome. Among them, 767 genes were differentially expressed (DEGs) after exposure to 9,10-PQ, which enriched 16 KEGG pathways. Among them, the glycolysis, phagosome, and FOXO signaling pathways were significantly activated in 9,10-PQ treatment compared with the control. These data indicate that 9,10-PQ increased the glycolysis capacity to produce more energy for resistance and harmed immune function. Moreover, several genes related to tumorigenesis were significantly up-regulated in response to 9,10-PQ, displaying the carcinogenic toxicity of 9,10-PQ to T. obscurus. Genes in steroid biosynthesis pathways were down-regulated in the 9,10-PQ treatment group, suggesting interference with the endocrine system. Overall, these findings provide information to help evaluate the environmental risks that oxygenated-PAHs present to T. obscurus.

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Investigation of the molecular mechanisms of hepatic injury upon naphthalene exposure in zebrafish (Danio rerio)

Cited by 10 publications

References 60 publications

Domestication and Temperature Modulate Gene Expression Signatures and Growth in the Australasian Snapper Chrysophrys auratus

Domestication and Temperature Modulate Gene Expression Signatures and Growth in the Australasian Snapper Chrysophrys auratus

Multilevel responses of adult zebrafish to crude and chemically dispersed oil exposure

Transcriptome changes ofTakifugu obscurusliver after acute exposure to the oxygenated-PAH 9,10-phenanthrenequione

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