Investigation of the microbial community in a microbiological additive used in a manure composting process

Wakase, Shiho; Sasaki, Hiraku; Itoh, Kazuyoshi; Otawa, Kenichi; Kitazume, Osamu; Nonaka, Jun; Satoh, Masaaki; Sasaki, Takayoshi; Nakai, Yutaka

doi:10.1016/j.biortech.2007.04.040

Cited by 58 publications

(20 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This indicates that the pyrene degraders enriched on agar plates and liquid cultures did not dominate pyrene degradation under conditions of non-water-saturation in soil, indicating the difficulty of inferring in situ PAH degradation potential from degradation activity of laboratory cultures. This is in line with previous findings for manure composting experiments where cultivable microorganisms did not dominate the composting process (Wakase et al 2008). Nevertheless, even though none of the reconstructed genomes could be affiliated with indicator genera from the soil-compost mixture, it should be noted that these genomes actually correspond to indicator genera appearing at day 46 in the fertilized-soil experiment.…”

Section: Discussionsupporting

confidence: 93%

Microbial communities in pyrene amended soil–compost mixture and fertilized soil

et al. 2017

View full text Add to dashboard Cite

Polycyclic aromatic hydrocarbons are distributed ubiquitously in the environment and form metabolites toxic to most organisms. Organic amendment of PAH contaminated soil with compost and farmyard manure has proven to be efficient for PAH bioremediation mediated by native microorganisms, even though information on the identity of PAH degraders in organic-amended soil is still scarce. Here we provide molecular insight into the bacterial communities in soil amended with compost or farmyard manure for which the degradation mass balances of 13C-labeled pyrene have been recently published and assess the relevant bacterial genera capable of degrading pyrene as a model PAH. We performed statistical analyses of bacterial genera abundance data based on total DNA and RNA (for comparison) extracted from the soil samples. The results revealed complex pyrene degrading communities with low abundance of individual degraders instead of a limited number of abundant key players. The bacterial degrader communities of the soil-compost mixture and soil fertilized with farmyard manure differed considerably in composition albeit showing similar degradation kinetics. Additional analyses were carried out on enrichment cultures and enabled the reconstruction of several nearly complete genomes, thus allowing to link microcosm and enrichment experiments. However, pyrene mineralizing bacteria enriched from the compost or unfertilized soil-compost samples did not dominate pyrene degradation in the soils. Based on the present findings, evaluations of PAH degrading microorganisms in complex soil mixtures with high organic matter content should not target abundant key degrading species, since the specific degraders may be highly diverse, of low abundance, and masked by high bacterial background.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-016-0306-9) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionsupporting

confidence: 93%

Microbial communities in pyrene amended soil–compost mixture and fertilized soil

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Wakase et al . (2008) reported that Lactobacillus was detected in many composting processes, was dominant in the early stages of composting and was capable of producing acetic acid. In this study, Lactobacillus was detected in the mesophilic stage of the three composting treatments, but it should be noted that three Lactobacillus species were still detected in the mature stage of the C group.…”

Section: Resultsmentioning

confidence: 99%

Effect of inoculation with a microbial consortium that degrades organic acids on the composting efficiency of food waste

Song

Zhang

Xia

et al. 2018

Microbial Biotechnology

View full text Add to dashboard Cite

SummaryIn order to overcome the excessive acidification problem, a microbial consortium for the degradation of organic acids (MCDOA), which acts synergistically in degrading organic acids, was developed and used as an inoculum to improve the efficiency of food waste composting. MCDOA could eliminate the initial lag phase of the pile temperature rise because of excessive acidification and effectively shorten the composting period. Fluorescence regional integration analysis of the excitation‐emission matrix spectra of dissolved organic matter showed that compared with raw material, in compost with MCDOA inoculation, the percent fluorescence response (P i,n) values of Regions I, II and IV decreased by 95.11%, 94.19% and 87.41%, respectively, and P i,n of Region V increased by 172.57%. The decreased and increased levels were markedly higher than in the two control groups (MgO and K2 HPO 4 treatment, and uninoculated compost). These findings revealed that MCDOA accelerated the degradation of proteinaceous compounds and the formation of complicated humic‐like materials. Bacterial profiles implied that MCDOA could improve the indigenous bacterial community structure and diversities of acetic and propionic acid‐degrading and lignin‐degrading bacteria, which might account for the high composting efficiency and degree of humification of the inoculated compost.

show abstract

“…Furthermore, the brightness of some bands in the two piles was different as observed. The brightness of band is an indicator for relative content of the present strains (Wakase et al, 2008). It should be noted that the brightness of certain bands in TM was stronger than that in CK, such as bands 5,8,13,14,15 and 18, which indicated that inoculation with enriched AOB could accelerate the growth and reproduction of some indigenous microorganisms.…”

Section: Dgge Analysis Of Bacterial Communitymentioning

confidence: 98%

A regulating method for reducing nitrogen loss based on enriched ammonia-oxidizing bacteria during composting

Yun

Zhao

Chen

et al. 2016

Bioresource Technology

105

View full text Add to dashboard Cite

Investigation of the microbial community in a microbiological additive used in a manure composting process

Cited by 58 publications

References 15 publications

Microbial communities in pyrene amended soil–compost mixture and fertilized soil

Microbial communities in pyrene amended soil–compost mixture and fertilized soil

Effect of inoculation with a microbial consortium that degrades organic acids on the composting efficiency of food waste

A regulating method for reducing nitrogen loss based on enriched ammonia-oxidizing bacteria during composting

Contact Info

Product

Resources

About