Investigation of the material properties of alginate for the development of hydrogel repair of dura mater

Nunamaker, Elizabeth A.; Otto, Kevin J.; Kipke, Daryl R.

doi:10.1016/j.jmbbm.2010.08.006

Cited by 47 publications

(36 citation statements)

References 69 publications

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“…If cells in this mass are indeed of meningeal origin, as our vimentin label indicates, it is possible that growth of this non-CNS tissue along and around implanted single or multishank implants may also affect electrode positions over time. In either case, a clearer understanding of the impact of a ‘healthy’ meningeal response to both craniotomy and intracortical implant would likely be informative to the development of increasingly biologically neutral intracortical MEAs (Nunamaker et al , 2011). …”

Section: Discussionmentioning

confidence: 99%

Chronic intracortical microelectrode arrays induce non-uniform, depth-related tissue responses

Woolley¹,

Desai²,

Otto³

2013

J. Neural Eng.

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Objective Brain-implanted microelectrode arrays show promise as future clinical devices. However, biological responses to various designs, compositions, and locations of these implants have not been fully characterized, and may impact the long term functionality of these devices. In order to improve our understanding of the tissue conditions at the interface of chronic brain-implanted microdevices, we proposed utilizing advanced histology and microscopy techniques to image implanted devices and surrounding tissue intact within brain slices. We then proposed utilizing these methods to examine whether depth within the cerebral cortex affected tissue conditions around implants. Approach Histological data was collected from rodent brain slices containing intact, intracortical microdevices 4 weeks after implantation surgery. Thick tissue sections containing the chronic implants were processed with fluorescent antibody labels, and imaged in an optical clearing solution using laser confocal microscopy. Main Results Tissue surrounding microdevices exhibited two major depth-related phenomena: a non-uniform microglial coating along the device length and a dense mass of cells surrounding the implant in cerebral cortical layers I and II. Detailed views of the monocyte-derived immune cells improve our understanding of the close and complex association that immune cells have with chronic brain-implants, and illuminated a possible relationship between cortical depth and the intensity of a chronic monocyte response penetrating microdevices. The dense mass of cells contained Vimentin, a protein typically expressed highly in non-CNS cells, evidence that non-CNS cells likely descended down the face of the penetrating devices from the pial surface. Significance Image data of highly non-uniform and depth-dependent biological responses along a device provides novel insight into the complexity of the tissue response to penetrating brain-implanted microdevices. The presented work also demonstrates the value of in situ histological collection of brain-implants for studying the complex tissue changes that occur, and the utility of pairing thick-tissue histology with appropriate optical clearing solutions.

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Section: Discussionmentioning

confidence: 99%

Chronic intracortical microelectrode arrays induce non-uniform, depth-related tissue responses

Woolley¹,

Desai²,

Otto³

2013

J. Neural Eng.

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“…In addition to chemical cross-linking, another class of hydrogels can be formed by the physical association of components, often by hydrogen-bonding at the molecular level. Examples of physically cross-linked hydrogels include alginate, agarose, Matrigel, collagen, and a recently described two-component protein hydrogel (Lee et al, 2001; Yang et al, 2009; Uemura et al, 2010; Nunamaker et al, 2011; Roberts et al, 2011). …”

Section: Polymers and Hydrogelsmentioning

confidence: 99%

Defining and designing polymers and hydrogels for neural tissue engineering

Aurand

Lampe

Bjugstad

2012

Neuroscience Research

167

201

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The use of biomaterials, such as hydrogels, as neural cell delivery devices is becoming more common in areas of research such as stroke, traumatic brain injury, and spinal cord injury. When reviewing the available research there is some ambiguity in the type of materials used and results are often at odds. This review aims to provide the neuroscience community who may not be familiar with fundamental concepts of hydrogel construction, with basic information that would pertain to neural tissue applications, and to describe the use of hydrogels as cell and drug delivery devices. We will illustrate some of the many tunable properties of hydrogels and the importance of these properties in obtaining reliable and consistent results. It is our hope that this review promotes creative ideas for ways that hydrogels could be adapted and employed for the treatment of a broad range of neurological disorders.

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“…Moreover, stiffness of ALG can be tuned in order to fit a range of mechanical property of various native tissues. The compressive modulus of ALG ranges from 1 to 1000 kPa, and the shear modulus of ALG varies from 0.02 to 40 kPa . Its stiffness can be modified by tuning several parameters, including polymer source, molecular weight, concentration, chemical modification, and types/density of crosslinking.…”

Section: Introductionmentioning

confidence: 99%

“…Cells in the ALG gel can be routinely analyzed by normal microscope, and the cells can be retrieved in a convenient way without damage. The most common manner to prepare ALG gel by crosslinking is to substitute the sodium ions from guluronic acid units with divalent cations such as calcium (Ca 2+ ), strontium (Sr 2+ ), zinc (Zn 2+ ), and barium (Ba 2+ ) . These ions have different affinities toward ALG, subsequently to modify gel stability, permeability and strength.…”

Section: Introductionmentioning

confidence: 99%

Investigation of cell viability and morphology in 3D bio‐printed alginate constructs with tunable stiffness

Shi

Laude

Yeong

2017

J Biomedical Materials Res

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In this article, mouse fibroblast cells (L929) were seeded on 2%, 5%, and 10% alginate hydrogels, and they were also bio-printed with 2%, 5%, and 10% alginate solutions individually to form constructs. The elastic and viscous moduli of alginate solutions, their interior structure and stiffness, interactions of cells and alginate, cell viability, migration and morphology were investigated by rheometer, MTT assay, scanning electron microscope (SEM), and fluorescent microscopy. The three types of bio-printed scaffolds of distinctive stiffness were prepared, and the seeded cells showed robust viability either on the alginate hydrogel surfaces or in the 3D bio-printed constructs. Majority of the proliferated cells in the 3D bio-printed constructs weakly attached to the surrounding alginate matrix. The concentration of alginate solution and hydrogel stiffness influenced cell migration and morphology, moreover the cells formed spheroids in the bio-printed 10% alginate hydrogel construct. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1009-1018, 2017.

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Investigation of the material properties of alginate for the development of hydrogel repair of dura mater

Cited by 47 publications

References 69 publications

Chronic intracortical microelectrode arrays induce non-uniform, depth-related tissue responses

Chronic intracortical microelectrode arrays induce non-uniform, depth-related tissue responses

Defining and designing polymers and hydrogels for neural tissue engineering

Investigation of cell viability and morphology in 3D bio‐printed alginate constructs with tunable stiffness

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