Investigation of the LCST of polyacrylamides as a function of molecular parameters and the solvent composition

Baltes, Thomas; Garret‐Flaudy, Frédéric; Freitag, Ruth

doi:10.1002/(sici)1099-0518(19990801)37:15<2977::aid-pola31>3.0.co;2-i

Cited by 145 publications

(127 citation statements)

References 16 publications

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“…[22][23][24][25][26] Below the T LCS the coiled conformation is favored, as this allows maximum interaction (strong hydrogen bonding) between the polymer and the water. With increasing temperature, the hydrogen bonding weakens, and concomitantly the entropycontrolled ''hydrophobic effect'' increases, hence the transition from a coil to a denser globule structure.…”

Section: Fluorescence Anisotropymentioning

confidence: 99%

“…[19,20] This scenario for the phase transition of PNIPA is further supported by Fourier Transform infrared (FT-IR) measurements in aqueous solution. [21] Numerous reports have appeared concerning the CGT of PDEA studied in detail using different techniques, such as LLS, [6,22,23] DSC, [24] NMR [25] and FT-IR. [26] Where applicable, the results are interpreted on the basis of a two state model to gain insight in the cooperativity of the transition.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of NaCl on the Conformational Behavior of Acenaphthylene Labeled Poly(N,N‐diethylacrylamide) in Dilute Aqueous Solution

Chen

Liu

Bian

et al. 2005

Macro Chemistry & Physics

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Summary: The effect of salts on the conformational behavior of acenaphthylene labeled poly(N,N‐diethylacrylamide) (PDEA/ACE) has been investigated in dilute aqueous solution using UV‐vis and fluorescence spectroscopy. It is demonstrated here that the effectiveness of various salts in changing the lower critical solution temperature (TLCS) of the PDEA/ACE solution followed the Hoffmeister series. The addition of NaCl linearly lowered the TLCS. In dilute aqueous solution, the PDEA/ACE adopted a loose coil conformation below its TLCS. The PDEA/ACE adopted a compact globule conformation above the temperature at which the coil to globule transition (CGT) was completed. A fluorescence anisotropy investigation suggested that a molten globule conformation existed during the CGT of the PDEA/ACE solution. In all of the above three conformations, the PDEA/ACE was more compact in the presence of NaCl than in the absence of any salt.

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Section: Fluorescence Anisotropymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Effect of NaCl on the Conformational Behavior of Acenaphthylene Labeled Poly(N,N‐diethylacrylamide) in Dilute Aqueous Solution

Chen

Liu

Bian

et al. 2005

Macro Chemistry & Physics

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“…The LCST of PNMAME in 0.9% NaCl and 0.9% KCl aqueous solution declined from 68°C to 49.2°C and from 68°C to 57.5°C, respectively. Freitag et al have also reported on the decreased tendency of LCST for PNIPAAm and poly(N, N-diethylacrylamide) in NaCl/(NH 4 ) 2 SO 4 -added solution [21]. NaCl and KCl showed the "salting-out" effect on the polymer solution and acted as water structure maker.…”

Section: Molecular Design and Characterization Of Pnmamementioning

confidence: 94%

Synthesis and characterization of a thermo-sensitive poly(N-methyl acryloylglycine methyl ester) used as a drug release carrier

Deng

Zhong

Jiao

et al. 2010

Front. Mater. Sci. China

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In this article, poly(N-methyl acryloylglycine methyl ester) (PNMAME) was prepared as a novel thermosensitive material with a lower critical solution temperature (LCST) at around 49.5°C. The chemical structures of the monomer NMAME and PNMAME were characterized by 1 H NMR and IR measurements. The LCST was investigated systematically as a function of PNMAME concentration, inorganic salt solution and pH value. The results indicated that LCST of PNMAME was obviously dependent on PNMAME concentration and pH. The LCST was increased with a decrease in pH value and PNMAME concentration. To obtain a thermo-sensitive hydrogel with the phase transition temperature close to human body temperature, the copolymerization was conducted between NMAME and N-acryloylglycine ethyl ester (NAGEE). The release behavior of caffeine was evaluated at different temperatures and contents of cross-linkers (N, N-methylenebis(acrylamide) (NMBA)). The increase of cross-linker content led to a decrease in the release rate of caffeine due to higher crossing density in the hydrogel network. In addition, a faster release of caffeine from the hydrogel with 3% NMBA at 37°C was found in contrast to that at 18°C.

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“…when bound to the MUC-1 peptide as well as the complexes containing the phage, the bioconjugate showed identical precipitation/redissolution behaviour as the polyNIPAAm-avidin alone. In the subsequent affinity precipitation experiments, a temperature of [20][21][22][23][24][25] C was used during phage-MUC-1 peptide-bioconjugate complex formation, while precipitations were done at 40 C, to assure quantitative recovery. Defining the redissolution temperature requires an optimization.…”

Section: Characterization Of the Stimuli-responsive Bioconjugatementioning

confidence: 99%

“…The polymer (polyNIPAAm) concentration has little influence on the value of the CST in the range of interest (0.1-5% w/v), but influences the abruptness and completeness of the precipitation. 12,22,23,24 Affinity precipitations in this report were all done at final polyNIPAAm concentrations of 2.0-3.5% (w/v) to assure abrupt and quantitative precipitation. In order to reduce the amount of expensive bioconjugate used in the experiments to that required for capturing the target molecule, the final polyNIPAAm concentration in the samples was adjusted via the addition of nonactivated polyNIPAAm.…”

Section: Characterization Of the Stimuli-responsive Bioconjugatementioning

confidence: 99%

Screening of a human antibody phage display library against a peptide antigen using stimuli‐responsive bioconjugates

Stocker

Dumoulin

Vandevyver

et al. 2008

Biotechnology Progress

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A synthetic antibody phage library (ETH‐2) was screened against the MUC‐1 peptide. Two standard methods were used, namely screening on immunotubes coated with the peptide antigen and on streptavidin‐coated paramagnetic particles together with a biotinylated MUC‐1 peptide. The results were compared with those obtained using a novel approach based on a stimuli‐responsive bioconjugate of avidin and poly‐(N‐isopropylacrylamide), also in combination with the biotinylated peptide. The poly‐(N‐isopropylacrylamide)‐based bioconjugate is soluble below a temperature of 30°C in the aqueous solutions pertinent to this study, but precipitates rapidly once this temperature is surpassed. The process is reversible, and the bioconjugate redissolves once the temperature is lowered again. The stimuli‐responsive bioconjugate was used to isolate specific phage from the library in several rounds of panning, consisting of repeated thermoprecipitation/redissolution cycles in a procedure known as “affinity precipitation.” As far as we know, this is the first time a compound as big as a viral particle has been specifically captured by a stimuli‐responsive bioconjugate. Compared to the two other investigated screening techniques, affinity precipitation of the phage led to a greater variety of genetically different specific phage, while the process was easy to handle and required no dedicated equipment safe for a centrifuge and a thermostated water bath.

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Investigation of the LCST of polyacrylamides as a function of molecular parameters and the solvent composition

Cited by 145 publications

References 16 publications

The Effect of NaCl on the Conformational Behavior of Acenaphthylene Labeled Poly(N,N‐diethylacrylamide) in Dilute Aqueous Solution

The Effect of NaCl on the Conformational Behavior of Acenaphthylene Labeled Poly(N,N‐diethylacrylamide) in Dilute Aqueous Solution

Synthesis and characterization of a thermo-sensitive poly(N-methyl acryloylglycine methyl ester) used as a drug release carrier

Screening of a human antibody phage display library against a peptide antigen using stimuli‐responsive bioconjugates

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