Investigation of the kinetic properties of Phyllanthus chamaepeuce Ridl. extracts for the inhibition of pancreatic lipase activity

Dechakhamphu, Ananya; Wongchum, Nattapong

doi:10.1016/j.hermed.2021.100508

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…In line with the sample-enzyme incubation period, measuring after the shortest color development time (15 min) resulted in the highest inhibition rate. In terms of method duration, our in-house PNLIP assay achieved similar or even faster [ 25 , 26 ] results in comparison to other studies. All in all, a 30 min total analysis time was enough to sensitively monitor orlistat inhibitory effect on PNLIP and the achieved optimized conditions were applied to monitor the anti-PNLIP effect of the selected plants (see Section 2.3 ).…”

Section: Resultssupporting

confidence: 56%

The In Vitro Inhibitory Effect of Selected Asteraceae Plants on Pancreatic Lipase Followed by Phenolic Content Identification through Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS)

Tsagkaris

Louckova

Jaegerova

et al. 2022

IJMS

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Pancreatic lipase (PNLIP, EC 3.1.1.3) plays a pivotal role in the digestion of dietary lipids, a metabolic pathway directly related to obesity. One of the effective strategies in obesity treatment is the inhibition of PNLIP, which is possible to be achieved by specific phenolic compounds occurring in high abundance in some plants. In this study, a multidisciplinary approach is presented investigating the PNLIP inhibitory effect of 33 plants belonging in the Asteraceae botanical family. In the first stage of the study, a rapid and cost-efficient PNLIP assay in a 96-microwell plate format was developed and important parameters were optimized, e.g., the enzyme substrate. Upon PNLIP assay optimization, aqueous and dichloromethane Asteraceae plant extracts were tested and a cut-off inhibition level was set to further analyze only the samples with a significant inhibitory effect (inhibitory rate > 40%), using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) method. Specifically, a metabolomic suspect screening was performed and 69 phenolic compounds were tentatively identified, including phenolic acids, flavonoids, flavonoid-3-O-glycosides, and flavonoid-7-O-glycosides, amongst others. In the case of aqueous extracts, phytochemicals known for inducing PNLIP inhibitory effect, e.g., compounds containing galloyl molecules or caffeoylquinic acids, were monitored in Chrysanthemum morifolium, Grindella camporum and Hieracium pilosella extracts. All in all, the presented approach combines in vitro bioactivity measurements to high-end metabolomics to identify phenolic compounds with potential medicinal and/or dietary applications.

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Section: Resultssupporting

confidence: 56%

The In Vitro Inhibitory Effect of Selected Asteraceae Plants on Pancreatic Lipase Followed by Phenolic Content Identification through Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS)

Tsagkaris

Louckova

Jaegerova

et al. 2022

IJMS

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“…To predict the combination modes of the ligand to PL and to analyze possible binding sites, software AutoDock Vina 1.1.2 was used [33]. Initially, the 3D conformer of isovitexin (PubChem CID 162350) was retrieved from a data bank as a ligand, and charge adjustment, rotational bonding detection, and rotational bonding setting were performed [34]. PL's 3D crystal structure (PDB code: 1LPB) was obtained from the PDB database (https://www.rcsb.org/) [35].…”

Section: Methodsmentioning

confidence: 99%

Inquiry lipaseoring the mechanism of pancreatic lipase inhibition by isovitexin based on multispectral method and enzyme inhibition assay

Yu,

Xing,

Jia

et al. 2024

Luminescence

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Isovitexin is a main natural flavonoid component in various plants. Currently, the inhibitory effect of isovitexin on pancreatic lipase (PL) and its mechanism have not been elucidated yet. In the present study, we investigated the inhibitory effect of isovitexin on PL, as well as its interaction mechanism, using enzyme inhibition methods, spectroscopic analysis, and molecular simulations. Results showed that isovitexin possessed significant PL inhibitory activity, with IC50 values of 0.26 ± 0.02 mM. The interaction between isovitexin and PL was dominated by static quenching, and mainly through hydrogen bonding and hydrophobic interaction forces. Analysis of fluorescence spectroscopy confirmed that isovitexin binding altered the conformation of the PL. Circular dichroism (CD) spectrum indicated that isovitexin altered the secondary structure of PL by decreasing the α‐helix content and increasing the β‐fold content. Molecular simulations further characterize the conformational changes produced by the interaction between isovitexin with PL. The performed study may provide a new insight into the inhibitory mechanism of isovitexin as a novel PL inhibitor.

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“…In in vitro studies, extracts of A. domesticus and T. molitor were able to inhibit pancreatic lipase [125]. These extracts could have an application in the treatment and prevention of obesity [217]. Proteins from the insects such as B. mori, T. molitor, Alphitobius diaperinus, and Gryllus bimaculatus [218][219][220][221] were able to inhibit angiotensin-converting enzyme (ACE), dipeptidyl peptidase-4 (DPP-IV) [220], and α-glucosidase activity [218,221], with antidiabetic potential.…”

Section: Appearance Of Traditional Insect Ingredients Bsfl Oil (A) An...mentioning

confidence: 98%

Perspective Chapter: Technological Strategies to Increase Insect Consumption – Transformation of Commodities Meal and Oil into Food/Functional Ingredients

Villanueva¹,

Ruiz²,

Valdés³

et al. 2023

Food Processing and Packaging Technologies - Recent Advances

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Insects have been proposed as an alternative source of nutrients to conventional foods, mainly protein sources because they have excellent nutritional quality and are sustainable. However, there are multiple barriers to mass consumption of insects, primarily the rejection and neophobia they provoke in individuals from Western cultures. Several studies have indicated that the acceptance of insects as food ingredients could be improved “if insects did not look like insects.” Therefore, the focus of current research is to transform commodity-type ingredients such as insect flour and oil through various technologies applied in the food industry such as protein concentration, encapsulation, hydrolysis, fermentation, deodorization, to develop food ingredients with better sensory and technological properties are better accepted by people as a part of their diet. Interestingly, some food ingredients obtained from insects also have functional properties that could increase interest in consumption. These aspects will be reviewed in this chapter for further consideration of insects as food ingredients of the future.

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Investigation of the kinetic properties of Phyllanthus chamaepeuce Ridl. extracts for the inhibition of pancreatic lipase activity

Cited by 6 publications

References 42 publications

The In Vitro Inhibitory Effect of Selected Asteraceae Plants on Pancreatic Lipase Followed by Phenolic Content Identification through Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS)

The In Vitro Inhibitory Effect of Selected Asteraceae Plants on Pancreatic Lipase Followed by Phenolic Content Identification through Liquid Chromatography High Resolution Mass Spectrometry (LC-HRMS)

Inquiry lipaseoring the mechanism of pancreatic lipase inhibition by isovitexin based on multispectral method and enzyme inhibition assay

Perspective Chapter: Technological Strategies to Increase Insect Consumption – Transformation of Commodities Meal and Oil into Food/Functional Ingredients

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