Investigation of the inside-outside distribution, intermembrane exchange and transbilayer movement of phospholipids in sonicated vesicles by shift reagent NMR

Barsukov, L.I.; Victorov, Alexander V.; Василенко, И. А.; Evstigneeva, R. P.; Bergelson, L.D.

doi:10.1016/0005-2736(80)90273-4

Cited by 80 publications

(37 citation statements)

References 23 publications

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“…The larger headgroup spacing for the negatively charged phosphatidylglycerol would clearly correspond to a lower energy situation. While such an asymmetric distribution of these two lipids in SUVs has previously been reported (Michaelson et al, 1973;Lentz et al, 1980), other workers have presented contradictory findings (Barsukov et al, 1980;Nordlund et al, 1981). This explanation is underminded, however, by two experimental observations.…”

Section: Discussionmentioning

confidence: 79%

Spontaneous vesiculation of large multilamellar vesicles composed of saturated phosphatidylcholine and phosphatidylglycerol mixtures

Tilcock¹,

Wong²,

Cullis³

1988

Biochemistry

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The influence of temperature and ionic strength on the vesiculation properties of large multilamellar vesicles containing various proportions of dimyristoylphosphatidylglycerol has been investigated. It is shown that at low ionic strengths preformed large multilamellar vesicles composed of dimyristoylphosphatidylcholine and dimyristoylphosphatidylglycerol (7:3) on incubation at the gel to liquid-crystalline transition temperature (Tc approximately 23 degrees C) spontaneously vesiculate to form predominantly unilamellar systems with a mean diameter of 120 nm. Such vesiculation is not observed for incubations at temperatures appreciably above or below Tc, and is also inhibited by higher ionic strengths. Stable large multilamellar vesicles are formed, however, in systems containing the dioleoyl species of phosphatidylcholine or phosphatidylglycerol and also for dimyristoylphosphatidylcholine/dimyristoylphosphatidylserine mixtures. The vesiculation properties of dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol mixtures, therefore, appear to reflect an instability in the region of the Tc driven by surface potential effects which are specific for the glycerol headgroup.

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Section: Discussionmentioning

confidence: 79%

Spontaneous vesiculation of large multilamellar vesicles composed of saturated phosphatidylcholine and phosphatidylglycerol mixtures

Tilcock¹,

Wong²,

Cullis³

1988

Biochemistry

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“…Since APT is sensitive to oxidative stress [47], ROS and/or oxidized PS might inhibit APT activity directly. Alternatively, oxidized PS (in contrast to non‐oxidized PS) might avoid APT‐dependent transport because of its poor recognition by the enzyme or because of its rapid flip‐flop movement across the membrane [48]. Further studies are required to reveal the exact relationships between PS oxidation and externalization.…”

Section: Discussionmentioning

confidence: 99%

Phosphatidylserine peroxidation/externalization during staurosporine‐induced apoptosis in HL‐60 cells

et al. 2002

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Although oxidative stress is commonly associated with apoptosis, its speci¢c role in the execution of the apoptotic program has yet to be described. We hypothesized that catalytic redox interactions between negatively charged phosphatidylserine (PS) and positively charged cytochrome c released into the cytosol, along with the production of reactive oxygen species (ROS), results in pronounced oxidation and externalization of PS, and subsequent recognition of apoptotic cells by macrophages. By using staurosporine, a protein kinase inhibitor that does not act as a prooxidant, we were able to induce apoptosis in HL-60 cells without triggering the confounding e¡ects of nonspeci¢c oxidation reactions. Through this approach, we demonstrated for the ¢rst time that PS underwent a statistically signi¢cant and pronounced oxidation at an early stage (2 h) of non-oxidant-induced apoptosis while the most abundant phospholipid, phosphatidylcholine, did not. Glutathione (GSH), the most abundant cytosolic thiol, also remained unoxidized at this time point. Furthermore, PS oxidation and the appearance of cytochrome c in the cytosol were concurrent; PS externalization was followed by phagocytosis of apoptotic cells. These ¢ndings are compatible with our proposed roles for oxidative PS-dependent signaling during apoptosis and phagocytosis. ß 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

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“…Indeed, several lines of indirect evidence suggest that there is induction of non-bilayer lipid in membranes following peroxidation. For example, the rate of trans-bilayer lipid migration increases (Barsukov et al, 1980;Shaw & Thompson, 1981). Also, it has been demonstrated that trace amounts of fatty acid hydroperoxide derivatives act as fusogens (Gast et al, 1982), and oxidized di-and trienoic fatty acids serve as Ca^"^ ionophores in model bilayers (Serhan et al, 1981).…”

Section: Age (Dlmentioning

confidence: 99%

The Role of Free Radicals in Senescence and Wounding

1987

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Summary Reactions involving free radicals are an inherent feature of plant senescence and appear to contribute to a process of oxidative deterioration that leads ultimately to cell death. Radical species derived from molecular oxygen are the primary mediators of this oxidative damage, but non‐radical excited states of oxygen, specifically singlet oxygen, may also be involved. Several lines of evidence suggest that degradation of lipids in senescing membranes and the ensuing release of free fatty acids initiate oxidative deterioration by providing substrate for lipoxygenase. In some tissues, lipoxygenase activity increases with advancing senescence in a pattern that is consistent with its putative role in promoting oxidative damage. However, there are important exceptions to this which may be explained by the fact that the timing and extent of peroxidative reactions initiated by lipoxygenase are likely to be determined more by the availability of substrate for the enzyme than by changes in its activity. There are both membranous and cytosolic forms of lipoxygenase in senescing tissues, and peroxidation of membrane lipids appears to be initiated by the membranous enzyme once the appropriate fatty acid substrates, linoleic acid and linolenic acid, become available. Since lipid peroxidation is known to form alkoxy and peroxy radicals as well as singlet oxygen, these reactions in membrane bilayers are probably a major source of activated oxygen species in senescing tissues. Further‐more, there are indications that activated oxygen from the lipoxygenase reaction can become substrate for the cytosolic form of the enzyme which, in turn, may raise the titre of activated oxygen during senescence. Additional possible sources of increased free radical production in senescing tissues include peroxidase, which shows greatly increased activity with advancing age, leakage of electrons from electron transport systems to oxygen, in particular from the photosynthetic electron transport system, and decompartmentalization of iron, which would facilitate formation of the highly reactive hydroxyl radical from the less reactive superoxide anion. A variety of macromolecules can be damaged by activated oxygen. Unsaturated fatty acids are especially prone to attack, and this implies that membranes are primary targets of free radical damage. The manifestations of this damage in senescing tissues range from altered membrane fluidity and phase properties to leakiness that can be attributed to a destabilized and highly perturbed membrane bilayer. There is also a progressive breakdown of cellular protein with advancing senescence. Free radicals can inactivate proteins by reacting with specific amino acid residues, and a number of in zitro studies have indicated that such alteration renders the proteins more prone to hydrolysis by proteases. Thus, although there is no direct evidence linking enhanced proteolysis during senescence to free radical damage, there is reason to believe that this may be a contributing factor. Wounding of certain plant ...

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Investigation of the inside-outside distribution, intermembrane exchange and transbilayer movement of phospholipids in sonicated vesicles by shift reagent NMR

Cited by 80 publications

References 23 publications

Spontaneous vesiculation of large multilamellar vesicles composed of saturated phosphatidylcholine and phosphatidylglycerol mixtures

Spontaneous vesiculation of large multilamellar vesicles composed of saturated phosphatidylcholine and phosphatidylglycerol mixtures

Phosphatidylserine peroxidation/externalization during staurosporine‐induced apoptosis in HL‐60 cells

The Role of Free Radicals in Senescence and Wounding

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