Investigation of the input-output relationship of engineered neural networks using high-density microelectrode arrays

Duru, Jens; Maurer, Benedikt; Giles Doran, Ciara; Jelitto, Robert; Küchler, Joël; Ihle, Stephan J.; Ruff, Tobias; John, Robert; Genocchi, Barbara; Vörös, János

doi:10.1016/j.bios.2023.115591

Cited by 6 publications

(6 citation statements)

References 52 publications

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“…We observed that neurons avoided regions patterned with PMOXA and preferentially grew on locations with PLL. While this patterning approach on MEAs is less effective than the use of microstructures, 23,24,43,44 our results serve as a proof-of-concept that surface patterns on MEAs can be induced in situ to introduce neural guidance at unprecedented spatial resolutions in vitro . Microstructures excel in the guidance of neurons due to their three-dimensional physical confinement of neurons.…”

Section: Discussionmentioning

confidence: 80%

Driving electrochemical reactions at the microscale using CMOS microelectrode arrays

Duru,

Rüfenacht,

Löhle

et al. 2023

Lab Chip

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Section: Discussionmentioning

confidence: 80%

Driving electrochemical reactions at the microscale using CMOS microelectrode arrays

Duru,

Rüfenacht,

Löhle

et al. 2023

Lab Chip

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“…In addition, the CO 2 level of the incubator can be held stable at 5 % without an external CO 2 source by using a 16 g CO 2 cartridge. The inkugo system was used in previous publications to transport cells from a campus with a cell culture facility to a campus with a scanning electron microscope (SEM) [27].…”

Section: Hardware In Contextmentioning

confidence: 99%

“…The inkubox system introduces a physiological environment that makes it feasible to record neural culture activity for multiple hours as opposed to only a few minutes without it. The system was used to generate data for previous publications [15,27]. In these works, neural cultures were maintained in commercial incubators and placed in inkubox several times per week for multiple hours for recording purposes.…”

Section: Validation and Characterizationmentioning

confidence: 99%

A modular and flexible open source cell incubator system for mobile and stationary use

Duru,

Maurer,

Ruff

et al. 2024

Preprint

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Culturing living cells in vitro requires the maintenance of physiological conditions for extended periods of time. Here, we introduce a versatile and affordable incubation system, addressing the limitations of traditional incubation systems. Conventionally, stationary cell incubators maintain constant temperature and gas levels for in vitro cell culturing. Combining such incubators with additional lab equipment proves challenging. The presented platform offers modularity and adaptability, enabling customization to diverse experimental needs. The system includes a main unit with a user-friendly interface as well as an interchangeable incubation chamber. We present two incubation chambers targeting two completely different use cases. The first chamber, named "inkugo" facilitates the transportation of cells for up to two hours without external power and for more than a day without an external CO2 source. The second chamber termed "inkubox" was designed to enable continuous electrophysiological recordings. Recordings from up to four neural cultures growing on high-density microelectrode arrays can be performed in parallel. The system's unique feature lies in its separability of control and incubation components, allowing one control unit to manage various custom chambers. The design's simplicity and the use of widely accessible components make the here proposed incubation system replicable for any laboratory. This platform fosters collaboration and experimentation in both decentralized and traditional laboratory settings, making it an invaluable addition to any cell culturing pipeline.

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“…Data were collected at a sampling frequency of 20 kHz, with a 10-bit resolution and a recording range of about ±3.2 mV, yielding a least significant bit (LSB) of 6.3 µV. Using customized software developed with the MaxWell Python API, we stored the raw traces and performed spike detection with a custom algorithm [55]. 13 3.6.4 Data analysis of spontaneous and stimulation-based electrical activity recordings Raw voltages traces were band-passed filtered (4th order acausal Butterworth filter, 300-3500 Hz).…”

Section: Measmentioning

confidence: 99%

Section: Measmentioning

confidence: 99%

An implantable biohybrid nerve model towards synaptic deep brain stimulation

Sifringer,

Fratzl,

Clément

et al. 2024

Preprint

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Restoring functional vision in blind patients lacking a healthy optic nerve requires bypassing retinal circuits, ideally, by directly stimulating the visual thalamus. However, available deep brain stimulation electrodes do not provide the resolution required for vision restoration. We developed an implantable biohybrid nerve model designed for synaptic stimulation of deep brain targets. The interface combines a stretchable stimulation array with an aligned microfluidic axon guidance system seeded with neural spheroids to facilitate the development of a 3 mm long nerve-like structure. A bioresorbable hydrogel nerve conduit was used as a bridge between the tissue and the biohybrid implant. We demonstrated stimulation of spheroids within the biohybrid structurein vitroand used high-density CMOS microelectrode arrays to show faithful activity conduction across the device. Finally, implantation of the biohybrid nerve onto the mouse cortex showed that neural spheroids grow axonsin vivoand remain functionally active for more than 22 days post-implantation.

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Investigation of the input-output relationship of engineered neural networks using high-density microelectrode arrays

Cited by 6 publications

References 52 publications

Driving electrochemical reactions at the microscale using CMOS microelectrode arrays

Driving electrochemical reactions at the microscale using CMOS microelectrode arrays

A modular and flexible open source cell incubator system for mobile and stationary use

An implantable biohybrid nerve model towards synaptic deep brain stimulation

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