Investigation of the influence of NaCl concentration on Halobacterium salinarum growth

Zeng, Chi; Zhu, Jiangfeng; Liu, Y.; Yang, Y.; Zhu, Jun-Cheng; Huang, Yifan; Shen, P

doi:10.1007/s10973-005-9988-y

Cited by 10 publications

(7 citation statements)

References 13 publications

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“…It has been found that the NaCl concentration is the most important factor in determining halobacterial growth. In addition, it has also been found that the NaCl requirement for halobacterial growth is specific and NaCl cannot be replaced by other solutes [39]. In the present study biomass and total carotenoid production by Halorubrum sp.…”

Section: Discussionsupporting

confidence: 51%

Optimization of Total Carotenoid Production by Halorubrum Sp. TBZ126 Using Response Surface Methodology

Hamidi¹

2014

Microb Biochem Technol

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Carotenoids are one of the most diverse and broadly distributed classes of pigments in nature with a high number of biotechnological applications. Carotenoids have a broad range of functions, especially in relation to human health and their role as biological antioxidants. The increasing demand for consumption of natural carotenoids has raised interest in their bio-production. The objective of the present study was the analysis of environmental factors (temperature, pH and salinity) through response surface methodology (RSM) on the total carotenoid production of Halorubrum sp. TBZ126. In addition the effect of light was evaluated. Five levels of temperature, pH, and salinity were selected based on central composite design (CCD) and RSM to reach the optimum values for the cell growth and carotenoid production. Bio-production was carried out in an orbital shaker using a 10% (v/v) inoculum, and agitation at 120 rpm for 9 days in a non-illuminated environment. Dry cell weight was determined and total carotenoid was estimated by spectrophotometer. The production of biomass ranged from 0.04 to 0.84 g/l and the total carotenoid from 0.15 to 10.78 mg/l. The optimum conditions for cell growth and total carotenoid production in Halorubrum sp. TBZ126 cultures, were temperature 31ºC and 32ºC, pH 7.51 and 7.94 and NaCl (w/v) 18.33% and 20.55%, respectively. In conclusion, employing RSM design and under the light exposure as an inducing factor, carotenoid production by Halorubrum sp. TBZ126 was elevated by 45%. Additionally, TBZ126 could produce carotenoids at lower concentrations of NaCl (as low as 2.5%), in the absence of sodium acetate without elevating magnesium sulfate concentration.

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Section: Discussionsupporting

confidence: 51%

Optimization of Total Carotenoid Production by Halorubrum Sp. TBZ126 Using Response Surface Methodology

Hamidi¹

2014

Microb Biochem Technol

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“…The Mg 2+ concentration remained unchanged since H. salinarum needs divalent ions for the integrity of its S-layer, which functions as the stable cell wall (Engelhardt 2007 , 2016 ). The strain showed apparently unimpaired growth down to 3 M NaCl but only minimal growth at 2.5 M. This result is in agreement with other observations (Zeng et al 2006 ). However, only H. salinarum in 3.5 M NaCl medium was indistinguishable from cells in control samples (4.3 M).…”

Section: Resultssupporting

confidence: 93%

Cryo-electron microscopy of an extremely halophilic microbe: technical aspects

et al. 2017

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Most halophilic Archaea of the class Halobacteriaceae depend on the presence of several molar sodium chloride for growth and cell integrity. This poses problems for structural studies, particularly for electron microscopy, where the high salt concentration results in diminished contrast. Since cryo-electron microscopy of intact cells provides new insights into the cellular and molecular organization under close-to-live conditions, we evaluated strategies and conditions to make halophilic microbes available for investigations in situ. Halobacterium salinarum, the test organism for this study, usually grows at 4.3 M NaCl. Adaptation to lower concentrations and subsequent NaCl reduction via dialysis led to still vital cells at 3 M salt. A comprehensive evaluation of vitrification parameters, thinning of frozen cells by focused-ion-beam micromachining, and cryo-electron microscopy revealed that structural studies under high salt conditions are possible in situ.Electronic supplementary materialThe online version of this article (doi:10.1007/s00792-016-0912-0) contains supplementary material, which is available to authorized users.

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“…By contrast, the similar N-linked pentasaccharide attached to glycoproteins of a second haloarchaea also originating from the Dead Sea, Haloarcula marismortui (optimal growth in 3.5–4 M NaCl [28]), is completely assembled on a single DolP carrier [29]. In yet another halophilic archaea, Halobacterium salinarum (optimal growth in 3.9 M NaCl [30]), the surface (S)-layer glycoprotein, the building block of the S-layer surrounding the cell, is simultaneously modified by two distinct N-linked glycans, one initially assembled on DolP, the second on DolPP [31,32]. In the thermoacidophile Sulfolobus acidocaldarius (optimal growth at 80°C and pH 2 [33]), DolPP is charged with the same hexasaccharide as N-linked to glycoproteins in this organism, as well as its derivatives, although DolP and hexose-charged DolP have also been detected [34,35].…”

Section: The Lipid Glycan Carriers Of N-glycosylation Across Evolumentioning

confidence: 99%

Lipid sugar carriers at the extremes: The phosphodolichols Archaea use in N-glycosylation

Eichler

Guan

2017

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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N-glycosylation, a post-translational modification whereby glycans are covalently linked to select Asn residues of target proteins, occurs in all three domains of life. Across evolution, the N-linked glycans are initially assembled on phosphorylated cytoplasmically-oriented polyisoprenoids, with polyprenol (mainly C55 undecaprenol) fulfilling this role in Bacteria and dolichol assuming this function in Eukarya and Archaea. The eukaryal and archaeal versions of dolichol can, however, be distinguished on the basis of their length, degree of saturation and by other traits. As is true for many facets of their biology, Archaea, best known in their capacity as extremophiles, present unique approaches for synthesizing phosphodolichols. At the same time, general insight into the assembly and processing of glycan-bearing phosphodolichols has come from studies of the archaeal enzymes responsible. In this review, these and other aspects of archaeal phosphodolichol biology are addressed.

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Investigation of the influence of NaCl concentration on Halobacterium salinarum growth

Cited by 10 publications

References 13 publications

Optimization of Total Carotenoid Production by Halorubrum Sp. TBZ126 Using Response Surface Methodology

Optimization of Total Carotenoid Production by Halorubrum Sp. TBZ126 Using Response Surface Methodology

Cryo-electron microscopy of an extremely halophilic microbe: technical aspects

Lipid sugar carriers at the extremes: The phosphodolichols Archaea use in N-glycosylation

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