Investigation of the individual human sulfatome in plasma and urine samples reveals an age-dependency

Correia, Mário S. P.; Thapa, Bhawana; Vujasinović, Miroslav; Löhr, J.-Matthias; Globisch, Daniel

doi:10.1039/d1ra05994g

Cited by 3 publications

(3 citation statements)

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“…12 The investigation of the human sulfatome is therefore of great importance for the elucidation of these metabolic exchanges. 13,14 The enzymatic hydrolysis of sulfated and glucuronidated metabolites is the most common method for their investigation and discovery. 14 metabolites (sulfatome analysis) and glucuronidated metabolites.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Sulfated metabolites have been identified as key regulators of microbiota–host interactions . The investigation of the human sulfatome is therefore of great importance for the elucidation of these metabolic exchanges. , …”

Section: Introductionmentioning

confidence: 99%

“… 12 The investigation of the human sulfatome is therefore of great importance for the elucidation of these metabolic exchanges. 13 , 14 …”

Section: Introductionmentioning

confidence: 99%

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Immobilized Enzymes on Magnetic Beads for Separate Mass Spectrometric Investigation of Human Phase II Metabolite Classes

Tsiara,

Riemer,

Correia

et al. 2023

Anal. Chem.

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The human body has evolved to remove xenobiotics through a multistep clearance process. Non-endogenous metabolites are converted through a series of phase I and different phase II enzymes into compounds with higher hydrophilicity. These compounds are important for diverse research fields such as toxicology, nutrition, biomarker discovery, doping control, and microbiome metabolism. One of the challenges in these research fields has been the investigation of the two major phase II modifications, sulfation and glucuronidation, and the corresponding unconjugated aglycon independently. We have now developed a new methodology utilizing an immobilized arylsulfatase and an immobilized β-glucuronidase to magnetic beads for treatment of human urine samples. The enzyme activities remained the same compared to the enzyme in solution. The separate mass spectrometric investigation of each metabolite class in a single sample was successfully applied to obtain the dietary glucuronidation and sulfation profile of 116 compounds. Our new chemical biology strategy provides a new tool for the investigation of metabolites in biological samples with the potential for broad-scale application in metabolomics, nutrition, and microbiome studies.

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