Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from South America on alkaloid extracts

Konrath, Eduardo Luis; Medeiros-Neves, Bruna; Lunardi, Paula; Passos, Carolina dos Santos; Simões-Pires, André; Ortega, Marı́a Gabriela; Gonçalves, Carlos Alberto; Cabrera, José Luis; Moreira, José Cláudio Fonseca; Henriques, Amélia Teresinha

doi:10.1016/j.jep.2011.10.042

Cited by 59 publications

(48 citation statements)

References 51 publications

(43 reference statements)

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“…Cytoprotective properties of club moss extracts were suggested in neurodegenerative processes via their antioxidant and anti-inflammatory properties. Moreover, it was indicated that alkaloid extracts from Lycopodium species inhibited acetylcholinesterase (AChE) activity in rat brain [28]. HupA is reported to increase efficiency of learning and memory in animals, and it was shown to be a promising treatment of Alzheimer's disease.…”

Section: Discussionmentioning

confidence: 99%

Original article Assessment of antioxidative activity of alkaloids from Huperzia selago and Diphasiastrum complanatum using in vitro systems

Czapski¹,

Szypuła²,

Kudlik³

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Original article Assessment of antioxidative activity of alkaloids from Huperzia selago and Diphasiastrum complanatum using in vitro systems

Czapski¹,

Szypuła²,

Kudlik³

et al. 2014

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“…Given the complex pathology of AD, current strategies for the development of new agents focus on compounds with various powers and plants are a major source of these compounds (Konrath et al, 2012).…”

Section: Concentrationmentioning

confidence: 99%

Antioxidant and anticholinesterase activities of the essential oil of Eugenia dysenterica DC.

Feitosa¹,

Barbosa²,

Melo³

et al. 2017

Afr. J. Pharm. Pharmacol.

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This paper describes the anticholinesterase and antioxidant activities of Eugenia dysenterica DC. (O.Berg. (Myrtaceae) essential oils from leaves (EOED). EOED were obtained by hydrodistillation using a Clevenger-type apparatus and the products were analyzed by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-flame ionization detector (GC-FID). The main constituents of EOED were caryophyllene oxide (66.3%), isoledene (3.9%), 1,3,8-p-menthatriene (3.5%), mustakone (3.46%), β-phellandrene (1.7%), and selin-11-en-4-α-ol (1.7%). The antioxidant assay was performed based on the formation of thiobarbituric acid reactive substances (TBARS), hydroxyl radical, and nitric oxide production. By performing the Ellman assay, it was observed that EOED was able to inhibit the enzyme acetylcholinesterase (AChE) with an IC 50 = 0.92 g.ml -1 promising better value when compared with the drug rivastigmine (IC 50 = 1.87 g.ml , it was found out that EOED prevented lipid peroxidation inhibiting amount of TBARS formed in a similar manner to ascorbic acid. In addition, there was a reduction in the production of hydroxyl radical as well as the production of nitric oxide. To the best of our knowledge, this is the first report on compounds from this species that have activity for potentially preventing neurodegenerative disorders.

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“…The total synthesis of lyconadin B has recently been described; this molecule is a member of the lycopodium alkaloid family of compounds, which are related in that they regulate various biological responses. [10][11][12] NGF, the molecule stimulated by lyconadin B, regulates the differentiation, survival, and growth of neurons and plays central role in neurological diseases such as Alzheimer and Parkinson diseases. Rescuing cholinergic neurons by inducing NGF is a promising strategy for the treatment of Alzheimer disease.…”

Section: Research-article2016mentioning

confidence: 99%

Morphological Evaluation of Nonlabeled Cells to Detect Stimulation of Nerve Growth Factor Expression by Lyconadin B

et al. 2016

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The success of drug development is greatly influenced by the efficiency of drug screening methods. Recently, phenotypebased screens have raised expectations, based on their proven record of identifying first-in-class drugs at a higher rate. Although fluorescence images are the data most commonly used in phenotype-based cell-based assays, nonstained cellular images have the potential to provide new descriptive information about cellular responses. In this study, we applied morphology-based evaluation of nonlabeled microscopic images to a phenotype-based assay. As a study case, we attempted to increase the efficiency of a cell-based assay for chemical compounds that induce production of nerve growth factor (NGF), using lyconadin B as a model compound. Because the total synthesis of lyconadin B was accomplished very recently, there is no well-established cell-based assay scheme for further drug screening. The conventional cell-based assay for evaluating NGF induction requires two types of cells and a total of 5 days of cell culture. The complexity and length of this assay increase both the risk of screening errors and the cost of screening. Our findings show that analysis of cellular morphology enables evaluation of NGF induction by lyconadin B within only 9 h.

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Investigation of the in vitro and ex vivo acetylcholinesterase and antioxidant activities of traditionally used Lycopodium species from South America on alkaloid extracts

Abstract: This study showed that the biological properties of the folk medicinal plants Lycopodium clavatum and Lycopodium thyoides include AChE inhibitory activity and antioxidant effects, two possible mechanisms of action in Alzheimer's related processes.

Cited by 59 publications

References 51 publications

Original article Assessment of antioxidative activity of alkaloids from Huperzia selago and Diphasiastrum complanatum using in vitro systems

Original article Assessment of antioxidative activity of alkaloids from Huperzia selago and Diphasiastrum complanatum using in vitro systems

Antioxidant and anticholinesterase activities of the essential oil of Eugenia dysenterica DC.

Morphological Evaluation of Nonlabeled Cells to Detect Stimulation of Nerve Growth Factor Expression by Lyconadin B

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