Investigation of the Impact of Cell Cycle Stage on Freeze Response Sensitivity of Androgen-Insensitive Prostate Cancer

Santucci, Kimberly L.; Baust, John M.; Snyder, Kristi K.; Buskirk, Robert G. Van; Baust, John G.

doi:10.1177/1533034616648059

Cited by 3 publications

(2 citation statements)

References 22 publications

(37 reference statements)

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“…This highlights the importance of first-line treatments that address a multitude of cancer’s adaptive mechanisms [ 25 ]. Adjunctive therapies utilizing freezing show great potential as a multifaceted approach [ 25 ], especially given that cryotherapy is non-selective for cell cycle stage [ 98 ], in contrast to RFA. Another major benefit to cryoablation is that it has been shown to launch a secondary immune response attacking metastatic disease [ 10 , 25 , 28 , 99 , 100 , 101 , 102 , 103 , 104 ].…”

Section: Discussionmentioning

confidence: 99%

An In Vitro Investigation into Cryoablation and Adjunctive Cryoablation/Chemotherapy Combination Therapy for the Treatment of Pancreatic Cancer Using the PANC-1 Cell Line

et al. 2022

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As the incidence of pancreatic ductal adenocarcinoma (PDAC) continues to grow, so does the need for new strategies for treatment. One such area being evaluated is cryoablation. While promising, studies remain limited and questions surrounding basic dosing (minimal lethal temperature) coupled with technological issues associated with accessing PDAC tumors and tumor proximity to vasculature and bile ducts, among others, have limited the use of cryoablation. Additionally, as chemotherapy remains the first-line of attack for PDAC, there is limited information on the impact of combining freezing with chemotherapy. As such, this study investigated the in vitro response of a PDAC cell line to freezing, chemotherapy, and the combination of chemotherapy pre-treatment and freezing. PANC-1 cells and PANC-1 tumor models were exposed to cryoablation (freezing insult) and compared to non-frozen controls. Additionally, PANC-1 cells were exposed to varying sub-clinical doses of gemcitabine or oxaliplatin alone and in combination with freezing. The results show that freezing to −10 °C did not affect viability, whereas −15 °C and −20 °C resulted in a reduction in 1 day post-freeze viability to 85% and 20%, respectively, though both recovered to controls by day 7. A complete cell loss was found following a single freeze below −25 °C. The combination of 100 nM gemcitabine (1.1 mg/m2) pre-treatment and a single freeze at −15 °C resulted in near-complete cell death (<5% survival) over the 7-day assessment interval. The combination of 8.8 µM oxaliplatin (130 mg/m2) pre-treatment and a single −15 °C freeze resulted in a similar trend of increased PANC-1 cell death. In summary, these in vitro results suggest that freezing alone to temperatures in the range of −25 °C results in a high degree of PDAC destruction. Further, the data support a potential combinatorial chemo/cryo-therapeutic strategy for the treatment of PDAC. These results suggest that a reduction in chemotherapeutic dose may be possible when offered in combination with freezing for the treatment of PDAC.

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Section: Discussionmentioning

confidence: 99%

An In Vitro Investigation into Cryoablation and Adjunctive Cryoablation/Chemotherapy Combination Therapy for the Treatment of Pancreatic Cancer Using the PANC-1 Cell Line

et al. 2022

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“…Temperature of the cancer area will be dropped below the freezing point owing to the injection of the liquid nitrogen. After the therapy, the temperature of that area returns to the normal temperature, however the normal cells on that area could survive [1,2]. Surviving of the normal cells at the extreme cold temperature motivate us to investigate the behavior of the cells at this kind of temperature.…”

Section: Introductionmentioning

confidence: 99%

Investigation on Thermodynamic Variables of the Enzyme During the Cryotherapy

2020

jcr

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In this paper, we present the thermodynamic behavior of an artificial enzyme when cells are affected by the cryotherapy. In order to illustrate the Gibbs free energy and the enthalpy of the enzyme at the extreme temperature, by using the concept of the thermodynamic laws and kinetics of the enzyme, we measure the thermodynamic variables of the ground state and the transition state of the specific artificial enzyme at the different temperatures during cryotherapy. The results can be used to explain that, cells could survive at the extreme temperature below the freezing point by changing the activation enthalpy and the enthalpy.

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Identification of a fundamental cryoinjury mechanism in MSCs and its mitigation through cell-cycle synchronization prior to freezing

Johnstone,

Gu,

Lin

et al. 2023

Cryobiology

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Investigation of the Impact of Cell Cycle Stage on Freeze Response Sensitivity of Androgen-Insensitive Prostate Cancer

Abstract: Although initial results suggested a potential sensitivity, PC-3 cells exposed to freezing as sorted populations did not reveal significant differences in cell death. As such, the data from this study suggest that there is no difference in cell cycle stage sensitivity to freezing injury.

Cited by 3 publications

References 22 publications

An In Vitro Investigation into Cryoablation and Adjunctive Cryoablation/Chemotherapy Combination Therapy for the Treatment of Pancreatic Cancer Using the PANC-1 Cell Line

An In Vitro Investigation into Cryoablation and Adjunctive Cryoablation/Chemotherapy Combination Therapy for the Treatment of Pancreatic Cancer Using the PANC-1 Cell Line

Investigation on Thermodynamic Variables of the Enzyme During the Cryotherapy

Identification of a fundamental cryoinjury mechanism in MSCs and its mitigation through cell-cycle synchronization prior to freezing

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