Investigation of the immunosuppressive activity of Physalin H on T lymphocytes

Yu, Youjun; Sun, Lijuan; Ma, Lei; Li, Jiyu; Hu, Lihong; Liu, Jianwen

doi:10.1016/j.intimp.2009.11.013

Cited by 40 publications

(28 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Previous studies have shown that withanolides have a potent immunosuppressive effect on other insects, inhibiting antibacterial activity, phagocytosis and hemocytic proliferation, and on human cells, suppressing T- and B-cell activation and proliferation222346. These effects may result from the ecdysteroid-modulating properties of withanolides discussed earlier.…”

Section: Discussionmentioning

confidence: 91%

See 1 more Smart Citation

Immune modulation enables a specialist insect to benefit from antibacterial withanolides in its host plant

et al. 2016

View full text Add to dashboard Cite

The development of novel plant chemical defenses and counter adaptations by herbivorous insect could continually drive speciation, producing more insect specialists than generalists. One approach to test this hypothesis is to compare closely related generalist and specialist species to reveal the associated costs and benefits of these different adaptive strategies. We use the specialized moth Heliothis subflexa, which feeds exclusively on plants in the genus Physalis, and its close generalist relative H. virescens. Specialization on Physalis plants necessitates the ability to tolerate withanolides, the secondary metabolites of Physalis species that are known to have feeding deterrent and immune inhibiting properties for other insects. Here we find that only H. subflexa benefits from the antibacterial properties of withanolides, and thereby gains a higher tolerance of the pathogen Bacillus thuringiensis. We argue that the specialization in H. subflexa has been guided to a large extent by a unique role of plant chemistry on ecological immunology.

show abstract

Section: Discussionmentioning

confidence: 91%

“…Withanolides are triterpenoids with ergostan-type steroids as core structures19. They have been shown to be potent feeding deterrent in other insect species2021, immunosuppressants2223, ecdysteroid antagonists24 and cytotoxic agents25. They also have antibacterial activity2627.…”

mentioning

confidence: 99%

Immune modulation enables a specialist insect to benefit from antibacterial withanolides in its host plant

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Since physalins induce immune depression in the R. prolixus hemocele (Castro et al, 2008Garcia et al, 2006), and in mammal cells (Jacobo-Herrera et al, 2006;Soares et al, 2003Soares et al, , 2006Vandenberghe et al, 2008;Vieira et al, 2005;Yu et al, 2010), we decided to investigate the immune responses in the insect gut, such as antibacterial activity and production of reactive nitrogen species. In our experiments, with physalin B topical and contact applications, the treated insects presented lower antibacterial activity than the infected control insects.…”

Section: Discussionmentioning

confidence: 99%

Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota

Castro

Moraes

Gonzalez

et al. 2012

Journal of Insect Physiology

View full text Add to dashboard Cite

Physalin B is a natural secosteroidal, extracted from the Solanaceae plant, Physalis angulata, and it presents immune-modulator effects on the bloodsucking bug, Rhodnius prolixus. In this work, R. prolixus was treated with physalin B at a concentration of 1 mg/ml of blood meal (oral application), or 20 ng/insect (applied topically) or 57 ng/cm(2) of filter paper (contact treatment), and infected with Trypanosoma cruzi Dm28c clone (2×10(6) epimastigotes/insect). The three types of applications significantly decreased the number of T. cruzi Dm28c in the gut comparing with the non-treated infected insects (controls). All groups of infected insects treated with physalin B had higher numbers of bacterial microbiota in the gut than the non-treated controls infected with T. cruzi. We observed that the infected physalin B insects with topical and contact treatments had a lower antibacterial activity in the gut when compared with control infected insects. Furthermore, infected insects with the physalin B oral treatment produced higher levels of nitrite and nitrate in the gut than control infected insects. These results demonstrate that physalin B decreases the T. cruzi transmission by inhibiting the parasite development in the insect vector R. prolixus. Herein the importance of physalin B modulation on the immune system and microbiota population in terms of parasite development and transmission are discussed.

show abstract

“…Pinto et al (33) , encontraron que la fisalina F induce cambios ultraestructurales en los núcleos y mitocondrias de linfocitos B periféricos, induciendo su apoptosis en pacientes con infección por HTLV-1. Yu et al (34) demostraron el efecto inmunosupresor de la fisalina H en células T en forma dosis-dependiente mediante la modulación del balance de células Th1/Th2 y la inducción de la producción de hemooxigenasa 1 en linfocitos T. Soares et al (35) demostraron la actividad supresora de las fisalinas B, F y G en la función linfocitaria e indican el uso potencial de las fisalinas como agentes inmunosupresores para los tratamientos de patologías en las cuales se desea la inhibición de la respuesta inmune.…”

Section: Gráfico 3 Variaciones De Los Porcentajes De Linfocitos Y Seunclassified

Actividad inmunoestimulante del extracto acuoso liofilizado de la planta entera de Physalis angulata L. en ratas albinas cepa Holtzman

Sisley¹,

Horna²,

Isern³

et al. 2017

Rev. per. med. integr.

View full text Add to dashboard Cite

inmunoestimulante del extracto acuoso liofilizado de la planta entera de Physalis Angulata L. en ratas albinas cepa Holtzman. Rev Peru Med Integrativa.2017;2(1):38-46. Historia del artículo ResumenObjetivo. Evaluar la actividad inmunoestimulante del extracto acuoso liofilizado (EAL) de la planta entera de Physalis angulata L. "bolsa mullaca". Materiales y métodos. Se usaron 32 ratas albinas divididas en cuatro grupos: agua destilada 3 mL (control); MetisopRinol (isoprinosine) 14,7 mg/kg; EAL Physalis angulata L. 8,6 mg/kg; y EAL Physalis angulata L. 17,2 mg/kg. El modelo de inmunoestimulación usado fue el de Delaveau et al. Se midió el porcentaje de linfocitos, neutrófilos segmentados; porcentaje de activación de la respuesta del sistema inmune (%ARSI) y absorbancia basal y a la 1 h, 3 h, 6 h y 24 h después de las intervenciones. Resultados. Se encontraron diferencias significativas en los valores de absorbancias cuando se realizó la comparación de todos los grupos de experimentación con el grupo control (p<0,001). Los grupos Physalis angulata 8,6 mg /kg y Physalis angulata 17,2 mg/kg presentaron sus %ARSI más altos a las seis horas de exposición (50,45 y 58,79% respectivamente). Los porcentajes de linfocitos y neutrófilos segmentados en los grupos isoprinosine 14,7 mg/kg, P. angulata 8,6 mg/kg, P. angulata 17,2 mg/kg fueron significativamente diferentes en comparación con los controles a las 3 h, 6 h y 24 h de intervención. Conclusiones. El EAL de Physalis angulata L, a dosis de 17,2 mg/kg mostró la mejor actividad inmunoestimulante, siendo quien más se acercó al efecto del isoprinosine 14,7 mg/kg. Palabras clave: Plantas Medicinales; Adyuvantes Inmunológicos; Physalis/efectos de drogas (Fuente: DeCS)Immunoestimulant activity of Physalis angulata L whole plant-lyophilized aqueous extract in albino Holtzman rats AbstractObjective. To evaluate immunostimulant activity of Physalis angulata L. "bolsa mullaca" whole plant aqueous lyophilized extract (EAL). Materials and Methods. We used 32 albino rats, which were divided in four groups: distillated water 3mL (control); Metisopinol (Isoprinosine) 14.7 mg/Kg; EAL Physalis angulata L. 8.6 mg/Kg; and EAL Physalis angulata L. 17.2 mg/Kg. The immunostimulant model of Delaveau et al was used. Lymphocites (%), Segmented Neutrophils (%), Activation percentage of immune system response (%ARSI) and basal absorbance were measured 1h, 3h, 6h and 24 h after the intervention. Results. Significate differences were found in absorbance's values when comparison between all experimental groups and control groups was made (p<0.001). The Physalis angulata 8.6 mg /Kg and Physalis angulata 17.2 mg/Kg groups presented their higher %ARSI at six hours after exposition (50.45% and 58.79%, respectively). The lymphocites (%) and segmented neutrophils (%) in Isoprinosine 14.7 mg/Kg, P. angulata 8.6 mg/kg and P. angulata 17.2 mg/kg groups had significate differences in comparison between control group at 3h, 6h and 24h after intervention. Conclusion. The Physalis angulata L EAL with a dose of 17....

show abstract

Investigation of the immunosuppressive activity of Physalin H on T lymphocytes

Cited by 40 publications

References 36 publications

Immune modulation enables a specialist insect to benefit from antibacterial withanolides in its host plant

Immune modulation enables a specialist insect to benefit from antibacterial withanolides in its host plant

Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota

Actividad inmunoestimulante del extracto acuoso liofilizado de la planta entera de Physalis angulata L. en ratas albinas cepa Holtzman

Contact Info

Product

Resources

About