Investigation of the Genotoxicity of Aluminum Oxide, β-Tricalcium Phosphate, and Zinc Oxide Nanoparticles In Vitro

Akbaba, Giray Buğra; Türkez, Hasan

doi:10.1177/1091581818775709

Cited by 22 publications

(15 citation statements)

References 52 publications

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“…In vitro, Al 2 O 3 NMs were found to induce MN in a concentration dependent manner after 24 h exposure from 0.5 µg/mL in Chinese hamster cells CHOK1 [19] and in human fibroblasts at 13.3 and 26.6 µg/cm 2 (50 and 100 µg/mL) [16]. In contrast, no MN increase was found in human blood lymphocytes [34] or RAW 264 macrophages exposed for 72 h to Al 2 O 3 NMs [35]. A negative response was also observed with the chromosome aberrations assay in human lymphocytes with Al 2 O 3 NMs [18].…”

Section: Discussionmentioning

confidence: 89%

Genotoxicity of Aluminum and Aluminum Oxide Nanomaterials in Rats Following Oral Exposure

et al. 2020

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Due to several gaps remaining in the toxicological evaluation of nanomaterials (NMs), consumers and public health agencies have shown increasing concern for human health protection. In addition to aluminum (Al) microparticles, Al-containing nanomaterials (Al NMs) have been applied by food industry as additives and contact materials. Due to the limited amount of literature on the toxicity of Al NMs, this study aimed to evaluate the in vivo genotoxic potential of Al 0 and Al 2 O 3 NMs after acute oral exposure. Male Sprague-Dawley rats were administered three successive gavages at 6, 12.5 and 25 mg/kg bw. A comparison with AlCl 3 was done in order to assess the potential effect of dissolution into Al ions. Both DNA strand breaks and oxidative DNA damage were investigated in six organs/tissues (duodenum, liver, kidney, spleen, blood and bone marrow) with the alkaline and the Fpg-modified comet assays. Concomitantly, chromosomal damage was investigated in bone marrow and colon with the micronucleus assay. The comet assay only showed DNA damage with Al 2 O 3 NMs in bone marrow (BM), while AlCl 3 induced slight but non-significant oxidative DNA damage in blood. No increase of chromosomal mutations was observed after treatment with the two Al MNs either in the BM or in the colons of rats.

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Section: Discussionmentioning

confidence: 89%

Genotoxicity of Aluminum and Aluminum Oxide Nanomaterials in Rats Following Oral Exposure

et al. 2020

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“…In the study of Yin, Casey [ 54 ], there were more micronuclei in the WIL2-NS cells subjected to 123 µmol/L medium- and large-sized ZnO NP (primary particle size: 78 and 147 nm, respectively), compared with the smallest particles (26 nm) and negative control. There were also increased micronuclei frequencies after ZnO NP exposure in lymphocyte cultures from heparinised human blood samples [ 71 ], THP-1 cells [ 51 ], human embryonic kidney (HEK293) cells, NIH/3T3 mouse embryonic fibroblast cells [ 49 ], WAG cells [ 50 ], and HaCaT cells [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Cellular Uptake and Toxicological Effects of Differently Sized Zinc Oxide Nanoparticles in Intestinal Cells

Mittag

Hoera

Kämpfe

et al. 2021

Toxics

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Due to their beneficial properties, the use of zinc oxide nanoparticles (ZnO NP) is constantly increasing, especially in consumer-related areas, such as food packaging and food additives, which is leading to an increased oral uptake of ZnO NP. Consequently, the aim of our study was to investigate the cellular uptake of two differently sized ZnO NP (<50 nm and <100 nm; 12–1229 µmol/L) using two human intestinal cell lines (Caco-2 and LT97) and to examine the possible resulting toxic effects. ZnO NP (<50 nm and <100 nm) were internalized by both cell lines and led to intracellular changes. Both ZnO NP caused time- and dose-dependent cytotoxic effects, especially at concentrations of 614 µmol/L and 1229 µmol/L, which was associated with an increased rate of apoptotic and dead cells. ZnO NP < 100 nm altered the cell cycle of LT97 cells but not that of Caco-2 cells. ZnO NP < 50 nm led to the formation of micronuclei in LT97 cells. The Ames test revealed no mutagenicity for both ZnO NP. Our results indicate the potential toxicity of ZnO NP after oral exposure, which should be considered before application.

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“…Informed consent forms were signed by each volunteer. The cell cultures were prepared according to our previous study (Akbaba and Türkez, 2018). The blood samples (0.5 mL) were cultured in 6 mL of culture medium (Chromosome Medium B, Biochrom, United Kingdom).…”

Section: Cell Culturesmentioning

confidence: 99%

In vitro genotoxicity assessment and comparison of cerium (IV) oxide micro- and nanoparticles

Arslan

Akbaba

2020

Toxicol Ind Health

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Cerium (IV) oxide (CeO2), which is used as a biomaterial, has wide application in areas such as the biomedical, glass polishing, electronic, automotive, and pharmacology industries. Comparing with the literature, in this study, the genotoxic effects of cerium (IV) oxide microparticles (COMPs) and cerium (IV) oxide nanoparticles (CONPs) were investigated for the first time in human peripheral blood cultures at concentrations of 0.78, 1.56, 3.125, 6.25, 12.5, 25, and 50 ppm for 72 h under in vitro conditions. Particle sizes of COMPs and CONPs were determined using scanning electron microscopic analysis. Micronucleus and chromosome aberration tests were used to determine the genotoxicity of COMPs and CONPs. The average particle sizes of COMPs and CONPs were approximately 148.25 and 25.30 nm, respectively. It was determined that CeO2 particles in both micro and nano sizes were toxic at all concentrations compared to the negative control group (distilled water). Importantly, COMPs and CONPs were genotoxic even at the lowest concentration (0.78 ppm). Comparing particle sizes, the data indicated that COMPs were more toxic than CONPs. The results suggest that genotoxicity of COMPs and CONPs may be a function of applied concentrations and particle sizes.

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Investigation of the Genotoxicity of Aluminum Oxide, β-Tricalcium Phosphate, and Zinc Oxide Nanoparticles In Vitro

Cited by 22 publications

References 52 publications

Genotoxicity of Aluminum and Aluminum Oxide Nanomaterials in Rats Following Oral Exposure

Genotoxicity of Aluminum and Aluminum Oxide Nanomaterials in Rats Following Oral Exposure

Cellular Uptake and Toxicological Effects of Differently Sized Zinc Oxide Nanoparticles in Intestinal Cells

In vitro genotoxicity assessment and comparison of cerium (IV) oxide micro- and nanoparticles

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