Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community

Abstract

"Investigation of the fate and effects of acetyl cedrene on Capitella teleta and sediment bacterial community" (2010). Valery Forbes Publications. 10. http://digitalcommons.unl.edu/biosciforbes/10 AbstractThe fate of the fragrance material, acetyl cedrene (AC), in sediment was examined in a 16 day laboratory experiment using the sediment microbial community subjected to the following treatments: AC (nominal concentration; 0 and 50 μg g −1 dw sediment) and macrofaunal worms (with/without Capitella teleta (formerly Capitella sp. I)). Furthermore effects of AC on microbial respiration in the system were determined by examining CO 2 flux. T-RFLP (terminal restriction fragment length polymorphism) was used to analyze PCR (polymerase chain reaction) amplified 16S DNA gene fragments from the sediments to detect changes in the structure and diversity of the bacterial community. In addition, survival of C. teleta in sediment was determined. Lastly, we examined how the interactions between microbes and C. teleta in the sediment affected the above-mentioned parameters. The results showed that there was an interaction between worm treatment and time of sampling on the loss of AC from the sediment. This was caused by AC loss initially being fastest in the sediment with C. teleta present, but at experimental termination there was no significant difference between the two treatments (i.e., with/ without worms) in the amount of AC remaining in the sediment. Survival of C. teleta was significantly reduced by AC at experimental termination, but neither microbial respiration nor structure and diversity of the bacterial community were significantly affected.

Bioaccumulation and Biotransformation of Triclosan and Galaxolide in the Freshwater Oligochaete Limnodrilus hoffmeisteri in a Water/Sediment Microcosm

“…Unlike HHCB, Dai et al found that after exposure for 14 days the level of sediment-associated acetyl cedrene (another fragrance material) was reduced by 88–99 and 13–31% in the sediment with and without C. teleta , respectively. However, another study reported that the level of acetyl cedrene in the sediment decreased by 72% in both treatments with and without C. teleta after 16 days . One explanation for these findings is that the microbial activity was very low initially in the study presented here due to the freezing of the sediment, which would potentially decrease the rate of microbial degradation compared to that of a fully active microbial community in previous studies.…”

“…However, another study reported that the level of acetyl cedrene in the sediment decreased by 72% in both treatments with and without C. teleta after 16 days. 68 One explanation for these findings is that the microbial activity was very low initially in the study presented here due to the freezing of the sediment, which would potentially decrease the rate of microbial degradation compared to that of a fully active microbial community in previous studies. In addition, it seems that macrofaunal biotransformation is both species-and chemical-specific (e.g., ref 63).…”

Ecological risks of personal care ingredients for subtropical benthic communities

“…Unlike HHCB, Dai et al (2012) found that sediment-associated acetyl cedrene (another fragrance material) was reduced by 88-99% and 13-31% in the sediment with and without C. teleta, respectively, after 14 days of exposure. However, another study reported that acetyl cedrene in the sediment decreased 72% in both treatments with and without C. teleta after 16 days (Ellegaard-Petersen et al, 2010). One explanation for these findings is that the microbial activity was very low initially in the present study due to the freezing of the sediment, which would potentially decrease microbial degradation compared to a full-active microbial community in previous studies.…”

When and How to Conduct Ecotoxicological Tests Using Natural Field‐Collected Sediment

“…Consequently, the use (e.g., for laboratory rearing and experiments) and preparation of natural field‐collected sediment are often developed and evolved locally within research groups. For instance, the ecotoxicology group at Roskilde University has been using natural field‐collected sediment when studying sediment‐associated contaminants over the past several decades (see Ellegaard‐Petersen et al, 2010; Linke‐Gamenick et al, 1999; Nielsen et al, 2017; Palmqvist et al, 2003; Sandgaard, Syberg, et al, 2023; Selck et al, 1998, 2003a; Thit et al, 2015).…”