Investigation of the expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes in different stage colorectal tumors

Söylemez, Zafer; Arikan, Evrim Suna; Solak, Mustafa; Arıkan, Yüksel; Tokyol, Çiğdem; Şeker, Hüseyin

doi:10.3906/sag-2010-18

Cited by 12 publications

(13 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The real-time RT-PCR technique was recently applied to measure expression levels of CPEB4 , APC , TRIP13 , EIF2S3 , EIF4A1 , IFNg , PIK3CA, and CTNNB1 genes in tumors and peripheral blood samples of colorectal cancer patients in stages I–IV of the disease [ 25 ]. Total RNA was extracted from tissues or peripheral blood samples, followed by reverse transcription to produce cDNA.…”

Section: Applicationsmentioning

confidence: 99%

“…Using specific primers for each gene, real-time PCR was then performed to analyze the mRNA level of each gene in colorectal cancer tissue specimens, colorectal cancer blood samples, normal colon tissues, and normal blood samples. The study concluded that TRIP13 and CPEB4 mRNA up-regulation in the peripheral blood of patients with colorectal cancer might be a potential target for an early-stage test of colorectal cancer [ 25 ]. Similarly, the real-time RT-PCR method was employed to determine and evaluate the microRNAs (miR-150, miR-146a, hsa-let-7e) expression profile within peripheral blood mononuclear cells (PBMCs) infected with the dengue virus.…”

Section: Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

Real-Time Polymerase Chain Reaction: Current Techniques, Applications, and Role in COVID-19 Diagnosis

Artika

Dewi

Nainggolan

et al. 2022

Genes

View full text Add to dashboard Cite

Successful detection of the first SARS-CoV-2 cases using the real-time polymerase chain reaction (real-time PCR) method reflects the power and usefulness of this technique. Real-time PCR is a variation of the PCR assay to allow monitoring of the PCR progress in actual time. PCR itself is a molecular process used to enzymatically synthesize copies in multiple amounts of a selected DNA region for various purposes. Real-time PCR is currently one of the most powerful molecular approaches and is widely used in biological sciences and medicine because it is quantitative, accurate, sensitive, and rapid. Current applications of real-time PCR include gene expression analysis, mutation detection, detection and quantification of pathogens, detection of genetically modified organisms, detection of allergens, monitoring of microbial degradation, species identification, and determination of parasite fitness. The technique has been used as a gold standard for COVID-19 diagnosis. Modifications of the standard real-time PCR methods have also been developed for particular applications. This review aims to provide an overview of the current applications of the real-time PCR technique, including its role in detecting emerging viruses such as SARS-CoV-2.

show abstract

Section: Applicationsmentioning

confidence: 99%

Section: Applicationsmentioning

confidence: 99%

Real-Time Polymerase Chain Reaction: Current Techniques, Applications, and Role in COVID-19 Diagnosis

Artika

Dewi

Nainggolan

et al. 2022

Genes

View full text Add to dashboard Cite

show abstract

“… Zhu et al (2019 ) found that TRIP13 was the most prominent differentially expressed AAA ATPase gene and a promising candidate oncogene in liver cancer. In addition, the TRIP13 mRNA level was upregulated in peripheral blood in colon cancer tissues, thus making it a potential target for early-stage diagnosis ( Soylemez et al, 2021 ). Nevertheless, no literature showed its association with stomach cancer, and further research is needed.…”

Section: Resultsmentioning

confidence: 99%

Leader gene identification for digestive system cancers based on human subcellular location and cancer-related characteristics in protein–protein interaction networks

Chen

Rong

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Stomach, liver, and colon cancers are the most common digestive system cancers leading to mortality. Cancer leader genes were identified in the current study as the genes that contribute to tumor initiation and could shed light on the molecular mechanisms in tumorigenesis. An integrated procedure was proposed to identify cancer leader genes based on subcellular location information and cancer-related characteristics considering the effects of nodes on their neighbors in human protein–protein interaction networks. A total of 69, 43, and 64 leader genes were identified for stomach, liver, and colon cancers, respectively. Furthermore, literature reviews and experimental data including protein expression levels and independent datasets from other databases all verified their association with corresponding cancer types. These final leader genes were expected to be used as diagnostic biomarkers and targets for new treatment strategies. The procedure for identifying cancer leader genes could be expanded to open up a window into the mechanisms, early diagnosis, and treatment of other cancer types.

show abstract

“…Понижение экспрессии генов MMD, TLR1, возможно, соотносится со снижением иммунной функции у пациентов с КРР [18,19]. Ген CPEB4, являющийся регулятором трансляции широкого спектра действия, может быть поразному экспрессирован на разных стадиях КРР [20], для построения модели 1 на нашей выборке имела значение его пониженная экспрессия.…”

Section: Discussionunclassified

Study of the expression profile of blood cells in patients with neoplasia of the lower gastrointestinal tract and evaluation of its diagnostic potential

et al. 2022

View full text Add to dashboard Cite

В работе представлены результаты разработки малоинвазивного метода дифференциальной диагностики колоректального рака (КРР) и полипоза на основе анализа транкриптома клеток периферической крови. Цель исследования. Определение профилей экспрессии отобранных по данным литературы генов, в клетках лейкоцитарной фракции (ЛФ) периферической крови у пациентов с КРР и полипами нижних отделов желудочно-кишечного тракта (ЖКТ), и создание на основе этого прогностической модели, позволяющей дифференцированно диагностировать КРР и полипоз. Материал и методы. В исследование были включены образцы периферической крови пациентов с диагнозами КРР (n=33) и полипоз (n=22), а также контрольной группы пациентов (n=30). Выделенную из ЛФ РНК использовали для изготовления библиотек с помощью набора RNAHyperPrep&HyperCap (Roche NimbleGen, США) с двойным обогащением таргетными последовательностями. NGS осуществляли на аппарате MiSeq (Illumina, США). Количество прочтений, выровнявшихся с исследуемыми генами, принимали за оценку уровня экспрессии. Результаты. Были выделены группы генов, дифференциально экспрессирующихся у пациентов с диагнозами КРР и полипоз и в контроле и построены модели логистической регрессии, позволяющие осуществить диагностику КРР, на основании анализа экспрессии генов FRMD3, ANXA3, DPEP1, CPEB4, MMD и TLR1 (точность 93,7%), и полипоза, на основании анализа экспрессии SERPINB5, CEACAM5, DPEP1 и STC1 (точность 84,6%) при сравнении с контрольной группой и отличить пациентов с диагнозом КРР от пациентов с диагнозом полипоз на основании анализа экспрессии генов MMD и MDM2 с точностью 78,3%. Заключение. В дальнейшем необходимо исследование экспрессии генов с наибольшим диагностическим потенциалом у пациентов независимой выборки методом цифровой ОТ-ПЦР и уточнение созданных нами моделей.

show abstract

Investigation of the expression levels of CPEB4, APC, TRIP13, EIF2S3, EIF4A1, IFNg, PIK3CA and CTNNB1 genes in different stage colorectal tumors

Cited by 12 publications

References 56 publications

Real-Time Polymerase Chain Reaction: Current Techniques, Applications, and Role in COVID-19 Diagnosis

Real-Time Polymerase Chain Reaction: Current Techniques, Applications, and Role in COVID-19 Diagnosis

Leader gene identification for digestive system cancers based on human subcellular location and cancer-related characteristics in protein–protein interaction networks

Study of the expression profile of blood cells in patients with neoplasia of the lower gastrointestinal tract and evaluation of its diagnostic potential

Contact Info

Product

Resources

About