Investigation of the expression and localization of glucose transporter 4 and fatty acid translocase/CD36 in equine skeletal muscle

Dam, Klien G. van; Breda, Eric van; Schaart, Gert; Ginneken, M. M. E. van; Wijnberg, Inge D.; Graaf-Roelfsema, Ellen de; Kolk, Johannes H. van der; Keizer, H. A.

doi:10.2460/ajvr.2004.65.951

Cited by 20 publications

(20 citation statements)

References 44 publications

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“…Since GLUT4 is predominantly expressed in insulin-sensitive tissues, it plays a crucial role in whole-body glucose homeostasis [24] and as a result is one of the most studied GLUT isoforms, including horses. The equine GLUT4 gene has been characterized in equine skeletal muscle, which shares a high degree of homology with that of other mammalians [17, 25–27]. Similar to other species, GLUT4 protein is abundantly expressed in striated muscle and adipose tissue of horses [27, 28].…”

Section: Regulation Of Glucose Transport In Healthy Statementioning

confidence: 99%

“…The equine GLUT4 gene has been characterized in equine skeletal muscle, which shares a high degree of homology with that of other mammalians [17, 25–27]. Similar to other species, GLUT4 protein is abundantly expressed in striated muscle and adipose tissue of horses [27, 28]. In addition, GLUT4 protein is expressed in equine muscle in a fiber-type specific manner with a higher expression in type 2B fibers, compared with that in type 1 fibers [27].…”

Section: Regulation Of Glucose Transport In Healthy Statementioning

confidence: 99%

“…Similar to other species, GLUT4 protein is abundantly expressed in striated muscle and adipose tissue of horses [27, 28]. In addition, GLUT4 protein is expressed in equine muscle in a fiber-type specific manner with a higher expression in type 2B fibers, compared with that in type 1 fibers [27]. In contrast, immunostaining of GLUT4 in the equine digital lamellae has produced inconclusive results [29, 30], while western blot of crude membrane and PCR showed that GLUT4 is not a predominant isoform, suggesting that this insulin-responsive GLUT is unlikely to play a substantial role in the regulation of lamellar glucose transport [17, 18].…”

Section: Regulation Of Glucose Transport In Healthy Statementioning

confidence: 99%

“…Although the proposed mechanisms involved in the pathogenesis of laminitis are numerous and interrelated, IR is at the forefront of current investigations [130–133]. To this end, the development of experimental models of hyperinsulinemia-induced laminitis in the horse has provided further insights into the pathogenesis of endocrinopathic laminitis, especially as it relates to the altered glucose metabolism [27, 131, 134]. This is particularly germane as the regulation of glucose transport in the lamellae in the healthy and laminitic horse remains scarce and controversial.…”

Section: Glucose Transporters: Implications For Metabolic Dysregulmentioning

confidence: 99%

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Expression and Regulation of Facilitative Glucose Transporters in Equine Insulin-Sensitive Tissue: From Physiology to Pathology

Lacombe

2014

ISRN Veterinary Science

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Glucose uptake is the rate-limiting step in glucose utilization in mammalians and is tightly regulated by a family of specialized proteins, called the facilitated glucose transporters (GLUTs/SLC2). GLUT4, the major isoform in insulin-responsive tissue, translocates from an intracellular pool to the cell surface and as such determines insulin-stimulated glucose uptake. However, despite intensive research over 50 years, the insulin-dependent and -independent pathways that mediate GLUT4 translocation are not fully elucidated in any species. Insulin resistance (IR) is one of the hallmarks of equine metabolic syndrome and is the most common metabolic predisposition for laminitis in horses. IR is characterized by the impaired ability of insulin to stimulate glucose disposal into insulin-sensitive tissues. Similar to other species, the functional capability of the insulin-responsive GLUTs is impaired in muscle and adipose tissue during IR in horses. However, the molecular mechanisms of altered glucose transport remain elusive in all species, and there is still much to learn about the physiological and pathophysiological functions of the GLUT family members, especially in regard to class III. Since GLUTs are key regulators of whole-body glucose homeostasis, they have received considerable attention as potential therapeutic targets to treat metabolic disorders in human and equine patients.

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Section: Regulation Of Glucose Transport In Healthy Statementioning

confidence: 99%

Section: Regulation Of Glucose Transport In Healthy Statementioning

confidence: 99%

Section: Regulation Of Glucose Transport In Healthy Statementioning

confidence: 99%

Section: Glucose Transporters: Implications For Metabolic Dysregulmentioning

confidence: 99%

See 2 more Smart Citations

Expression and Regulation of Facilitative Glucose Transporters in Equine Insulin-Sensitive Tissue: From Physiology to Pathology

Lacombe

2014

ISRN Veterinary Science

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“…The location of insulinsensitive glucose transporters GLUT-4 within intracellular storage pools and in their active position within the sarcolemma in equine muscle has been characterized by this method 54,55 . The location of insulinsensitive glucose transporters GLUT-4 within intracellular storage pools and in their active position within the sarcolemma in equine muscle has been characterized by this method 54,55 .…”

Section: Immunofluorescent and Immunohistochemical Stainsmentioning

confidence: 99%

Muscle energetics in exercising horses

Votion

Navet

Lacombe

et al. 2007

Equine Comp. Exerc. Physiol.

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An optimally functional musculoskeletal system is crucial for athletic performance and even minor perturbations can limit athletic ability. The introduction of the muscle biopsy technique in the 1970s created a window of opportunity to examine the form and function of equine skeletal muscle. Muscle histochemical and biochemical analyses have allowed characterization of the properties of equine muscle fibres and their influence on, and adaptation to, physical exertion. Analyses of exercise responses during standardized treadmill exercise and field studies have illustrated the role of cellular energetics in determining athletic suitability for specific disciplines, mechanisms of fatigue, adaptations to training and the affect of diet on metabolic responses. This article provides a review of the tools available to study muscle energetics in the horse, discusses the muscular metabolic pathways and summarizes the energetics of exercise.qualitative assessment of the effects of exercise, training and dietary manipulation on substrate metabolism at the level of the muscle and the whole body. Muscle biopsy techniqueThe percutaneous needle muscle biopsy technique was introduced to equine research by Lindholm and Piehl 4 as well as by Snow and Guy 5 . Since that time, it has proved to be an invaluable tool in defining the histological, histochemical and biochemical properties of equine skeletal muscle. Standardization of the site of the muscle biopsy is imperative because equine skeletal muscles have a heterogeneous distribution of muscle fibre types within the muscle 6 . Deeper regions within locomotory muscles have contractile and metabolic characteristics similar to those of postural muscles 7-9 . In addition, fibre types vary among different muscles in the same horses as well as across horses and breeds [10][11][12][13][14][15][16][17] . The selection of the specific muscle to biopsy therefore is of critical importance and will depend on its propulsive or postural role. Many studies of equine athletes utilize the gluteus medius muscle or the semitendinosus muscle because of its importance in locomotion and demonstrated metabolic adaptations to exercise and training [18][19][20][21][22][23][24][25][26] . Other investigators have also used the triceps brachii, along with the masseter 26 (as a non-exercise muscle control sample site).When sampling site and depth are consistent and potentially involve several sites 21,23,24,27 , repeatable results are obtained 28 . Muscle biopsy has provided a wealth of information regarding the histochemical, biochemical and metabolic properties of various muscles within the same horse 9,29,30 , between horses 11 and breeds 15,21 as well as responses to exercise 25,31,32 . A detailed review of the technique for performing percutaneous needle muscle biopsy and sample preparation can be obtained elsewhere [3][4][5]21,22,33 .

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Endocrine and metabolic physiology

Geor¹

2013

Equine Applied and Clinical Nutrition

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Investigation of the expression and localization of glucose transporter 4 and fatty acid translocase/CD36 in equine skeletal muscle

Abstract: In equine skeletal muscle, GLUT4 and FAT/CD36 are expressed in a fiber type selective manner.

Cited by 20 publications

References 44 publications

Expression and Regulation of Facilitative Glucose Transporters in Equine Insulin-Sensitive Tissue: From Physiology to Pathology

Expression and Regulation of Facilitative Glucose Transporters in Equine Insulin-Sensitive Tissue: From Physiology to Pathology

Muscle energetics in exercising horses

Endocrine and metabolic physiology

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