Investigation of the estrogenic activities of pesticides from Pal-dang reservoir by in vitro assay

Oh, Young Jin; Jung, Yeon Jung; Kang, Joon Wun; Yoo, Young Sook

doi:10.1016/j.scitotenv.2007.07.013

Cited by 18 publications

(3 citation statements)

References 30 publications

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“…et al also reported that flutolanil may alter the estrogen endocrine system, including inducing MCF-7BUS cell proliferation, increasing the number of cells, and decreasing ERα protein levels. 7 Previous literatures demonstrated that flutolanil can cause acute toxicity in fish after 4 days of exposure with a median lethal concentration (LC 50 ) at 6.3 mg/L in killifish and at 2.70 mg/L in adult zebrafish. 5,8 In addition, an acute exposure of flutolanil is harmful to zebrafish embryonic survive and development.…”

Section: Introductionmentioning

confidence: 99%

Chronic Toxic Effects of Flutolanil on the Liver of Zebrafish (Danio rerio)

Teng

Zhou

Song³

et al. 2019

Chem. Res. Toxicol.

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Flutolanil is a broad-spectrum amide fungicide that is widely used to prevent fungal pathogens in agriculture. However, its usage may have a potential environmental impact on organisms. So far, few literatures have investigated the chronic toxicity of flutolanil at concentrations relevant to environmental conditions in the nontarget aquatic organisms. This study was aimed at evaluating whether the long-term exposure of flutolanil affects oxidative stress, immune response, and apoptosis in the liver of zebrafish (Danio rerio). The results showed that the activity of catalase (CAT) was significantly decreased in the liver in all flutolanil-treated groups. Interestingly, the malondialdehyde (MDA) contents were remarkably increased following the flutolanil exposure. Deoxyribonucleic acid (DNA) damage was increased with a concentration-dependent manner. The transcription level of genes involved in apoptosis and the immune system were significantly altered following flutolanil chronic exposure in zebrafish liver. Furthermore, the caspase-3 enzyme activity was significantly increased. Taken together, this study demonstrated that the resulting effects on oxidative stress, immune toxicity, and apoptosis may be responsible for the pathological alterations in zebrafish liver after flutolanil exposure at concentrations relevant to environmental conditions, advancing the knowledge of pesticide environmental risk assessment.

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Section: Introductionmentioning

confidence: 99%

Chronic Toxic Effects of Flutolanil on the Liver of Zebrafish (Danio rerio)

Teng

Zhou

Song³

et al. 2019

Chem. Res. Toxicol.

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“…Following exposure to flutolanil at 6.18 µM, Teng et al [5] observed that 72 hpf embryos failed to hatch and showed severe morphological defects, including pericardial edema, spine curvature, and shortened tail. In addition, flutolanil at 1.57 µM induced slower heartbeat, increased accumulation of thyroid hormones T3 and T4, and expression changes of several genes involved in thyroid hormone transport and regulation: trh, tsrh, tpo, dio1, and tra.…”

Section: Developmental Toxicity Of Sdhi Fungicidesmentioning

confidence: 99%

“…According to manufacturers' recommendations, the concentrations of SDHI solutions spread on fields should range from 0.5 to 2 mM, and the initial concentration of thifluzamide in paddy water after spraying is 7.4 µM [4]. In addition, the increasing use of SDHI fungicides worldwide results in these substances being frequently detected in aquatic environments [5], sometimes at concentrations exceeding reported toxic levels (e.g., 0.72 µM flutolanil in effluent water in Japan [6] and 0.1 µM boscalid in coastal estuary water in California [7]).…”

Section: Introductionmentioning

confidence: 99%

SDHI Fungicide Toxicity and Associated Adverse Outcome Pathways: What Can Zebrafish Tell Us?

Yanicostas

Soussi-Yanicostas

2021

IJMS

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Succinate dehydrogenase inhibitor (SDHI) fungicides are increasingly used in agriculture to combat molds and fungi, two major threats to both food supply and public health. However, the essential requirement for the succinate dehydrogenase (SDH) complex—the molecular target of SDHIs—in energy metabolism for almost all extant eukaryotes and the lack of species specificity of these fungicides raise concerns about their toxicity toward off-target organisms and, more generally, toward the environment. Herein we review the current knowledge on the toxicity toward zebrafish (Brachydanio rerio) of nine commonly used SDHI fungicides: bixafen, boscalid, fluxapyroxad, flutolanil, isoflucypram, isopyrazam, penthiopyrad, sedaxane, and thifluzamide. The results indicate that these SDHIs cause multiple adverse effects in embryos, larvae/juveniles, and/or adults, sometimes at developmentally relevant concentrations. Adverse effects include developmental toxicity, cardiovascular abnormalities, liver and kidney damage, oxidative stress, energy deficits, changes in metabolism, microcephaly, axon growth defects, apoptosis, and transcriptome changes, suggesting that glycometabolism deficit, oxidative stress, and apoptosis are critical in the toxicity of most of these SDHIs. However, other adverse outcome pathways, possibly involving unsuspected molecular targets, are also suggested. Lastly, we note that because of their recent arrival on the market, the number of studies addressing the toxicity of these compounds is still scant, emphasizing the need to further investigate the toxicity of all SDHIs currently used and to identify their adverse effects and associated modes of action, both alone and in combination with other pesticides.

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Online solid phase extraction LC–MS/MS method for the analysis of succinate dehydrogenase inhibitor fungicides and its applicability to surface water samples

2014

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A sensitive and selective analytical method, based on online solid phase extraction coupled to LC-MS/MS, was developed and validated to determine traces of several recently introduced fungicides in surface water and wastewater. The list of target analytes included eight succinate dehydrogenase inhibitors (bixafen, boscalid, fluopyram, flutolanil, fluxapyroxad, isopyrazam, penflufen, and penthiopyrad), and two other fungicides with different modes of action, fenpyrazamine and fluopicolide. Detection and quantification limits in various matrices were in the range of 0.1 to 2 and 0.5 to 10 ng/L, respectively. Moderate signal suppression was observed in surface water (≤15%) and wastewater (≤25%) and was well compensated by the selected internal standard. The intra- and inter-day precisions were generally <10 and <20%, respectively. The applicability of the method was demonstrated in a study on the occurrence of fungicides in the river Glatt, Switzerland, that drains a catchment area of 419 km(2) with a substantial proportion of agricultural land. Of the studied compounds, only boscalid and fluopicolide were detected in flow-proportional weekly composite samples, generally at low concentrations up to 15 and 5 ng/L, respectively. While fluopicolide was detected in only 30% of the samples above the LOD of 0.5 ng/L, boscalid was detected in all samples analyzed between March and October 2012.

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Investigation of the estrogenic activities of pesticides from Pal-dang reservoir by in vitro assay

Cited by 18 publications

References 30 publications

Chronic Toxic Effects of Flutolanil on the Liver of Zebrafish (Danio rerio)

Chronic Toxic Effects of Flutolanil on the Liver of Zebrafish (Danio rerio)

SDHI Fungicide Toxicity and Associated Adverse Outcome Pathways: What Can Zebrafish Tell Us?

Online solid phase extraction LC–MS/MS method for the analysis of succinate dehydrogenase inhibitor fungicides and its applicability to surface water samples

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