Investigation of the effects of catharanthine and Q10 on Nrf2 and its association with MMP-9, MRP1, and Bcl-2 and apoptosis in a model of hepatocellular carcinoma

Heidari-Kalvani, Nafiseh; Alizadeh-Fanalou, Shahin; Yarahmadi, Sahar; Fallah, Sudabeh; Alipourfard, Iraj; Farahmandian, Navid; Barjesteh, Fereshteh; Bahreini, Elham

doi:10.1007/s00210-023-02767-0

Cited by 2 publications

(1 citation statement)

References 67 publications

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“…In a rat temozolomide-resistant glioma cell line, the co-administration of CoQ10 increased the efficacy of temozolomide in reducing cell proliferation and invasiveness [87]. In a hepatocellular carcinoma cell line, Heidari-Kalvani et al [88] reported that CoQ10 administration reduced Nrf2 (nuclear factor erythroid-2related factor 2) levels, matrix metalloproteinase activity, and metastatic potential, but induced apoptosis. Finally, Frontiñán-Rubio et al [72] found that CoQ10 supplementation reduced glioblastoma growth and infiltration capacity in xenografts via proteome remodelling and the inhibition of angiogenesis and inflammation.…”

Section: Cell Culture Studiesmentioning

confidence: 99%

Energy Metabolism as a Therapeutic Target in Cancer: The Role of Coenzyme Q10

Mantle,

Rowbottom,

Jones

et al. 2024

Oxygen

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The generation of energy within cells is a fundamental process enabling cell survival, and as such it represents a potential target in cancer therapy. In this article, we therefore review the relative contributions of glycolysis and oxidative phosphorylation/mitochondrial function to cancer cell energy generation, and we highlight their respective potential value as chemotherapeutic targets. This article is particularly focussed on the potential role of coenzyme Q10 in the prevention and treatment of cancer.

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Section: Cell Culture Studiesmentioning

confidence: 99%

Energy Metabolism as a Therapeutic Target in Cancer: The Role of Coenzyme Q10

Mantle,

Rowbottom,

Jones

et al. 2024

Oxygen

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Probiotic-derived silver nanoparticles target mTOR/MMP-9/BCL-2/dependent AMPK activation for hepatic cancer treatment

Elmetwalli,

Abdel-Monem,

El-Far

et al. 2024

Med Oncol

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Recent advances in nanotechnology have offered novel ways to combat cancer. By utilizing the reducing capabilities of Lactobacillus acidophilus, silver nanoparticles (AgNPs) are synthesized. The anti-cancer properties of AgNPs have been demonstrated in previous studies against several cancer cell lines; it has been hypothesized that these compounds might inhibit AMPK/mTOR signalling and BCL-2 expression. Consequently, the current research used both in vitro and in silico approaches to study whether Lactobacillus acidophilus AgNPs could inhibit cell proliferation autophagy and promote apoptosis in HepG2 cells. The isolated strain was identified as Lactobacillus acidophilus strain RBIM based on 16 s rRNA gene analysis. Based on our research findings, it has been observed that this particular strain can generate increased quantities of AgNPs when subjected to optimal growing conditions. The presence of silanols, carboxylates, phosphonates, and siloxanes on the surface of AgNPs was confirmed using FTIR analysis. AgNPs were configured using UV–visible spectroscopy at 425 nm. In contrast, it was observed that apoptotic cells exhibited orange-coloured bodies due to cellular shrinkage and blebbing initiated by AgNP treatment, compared to non-apoptotic cells. It is worth mentioning that AgNPs exhibited remarkable selectivity in inducing cell death, specifically in HepG2 cells, unlike normal WI-38 cells. The half-maximum inhibitory concentration (IC50) values for HepG2 and WI-38 cells were 4.217 µg/ml and 154.1 µg/ml, respectively. AgNPs induce an upregulation in the synthesis of inflammation-associated cytokines, including (TNF-α and IL-33), within HepG2 cells. AgNPs co-treatment led to higher glutathione levels and activating pro-autophagic genes such as AMPK.Additionally, it resulted in the suppression of mTOR, MMP-9, BCL-2, and α-SMA gene expression. The docking experiments suggest that the binding of AgNPs to the active site of the AMPK enzyme leads to inhibiting its activity. The inhibition of AMPK ultimately results in the suppression of the mechanistic mTOR and triggers apoptosis in HepG2 cells. In conclusion, the results of our study indicate that the utilization of AgNPs may represent a viable strategy for the eradication of liver cancerous cells through the activation of apoptosis and the enhancement of immune system reactions.

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Investigation of the effects of catharanthine and Q10 on Nrf2 and its association with MMP-9, MRP1, and Bcl-2 and apoptosis in a model of hepatocellular carcinoma

Cited by 2 publications

References 67 publications

Energy Metabolism as a Therapeutic Target in Cancer: The Role of Coenzyme Q10

Energy Metabolism as a Therapeutic Target in Cancer: The Role of Coenzyme Q10

Probiotic-derived silver nanoparticles target mTOR/MMP-9/BCL-2/dependent AMPK activation for hepatic cancer treatment

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