Investigation of the effects of microalgal cell concentration and electroporation, microwave and ultrasonication on lipid extraction efficiency

Garoma, Temesgen; Janda, Danielle

doi:10.1016/j.renene.2015.08.009

Cited by 54 publications

(18 citation statements)

References 29 publications

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“…Membrane lipids and carbohydrates were extracted from the breast cell membranes according to previously reported method [70–72]. For membrane proteins extraction, the breast cell suspension (5 × 10 6 cells) was centrifuged at 1000 rpm for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Tao

Auguste

2017

Biomaterials

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Early detection of breast cancer is a critical component in patient prognosis and establishing effective therapy regimens. Here, we developed an easily accessible yet potentially powerful sensor to detect cancer cell targets by utilizing seven dual-ligand cofunctionalized gold nanoclusters (AuNCs) as both effective cell recognition elements and signal transducers. On the basis of this AuNC multichannel sensor, we have successfully distinguished healthy, cancerous and metastatic human breast cells with excellent reproducibility and high sensitivity. Triple negative breast cancer cells (TNBCs), which exhibit low expression of the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor-2, were identified. The high accuracy of the blind breast cell sample tests further validates the practical application of the sensor array. In addition, the versatility of the sensor array is further justified by identifying amongst distinct cell types, different cell concentrations and cell mixtures. Notably, the drug-resistant cancer cells can also be efficiently discriminated. Furthermore, the dual-ligand cofunctionalized AuNCs can efficiently differentiate different cells from the peripheral blood of tumor-free and tumor-bearing mice. Taken together, this fluorescent AuNCs based array provides a powerful cell analysis tool with potential applications in biomedical diagnostics.

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Section: Methodsmentioning

confidence: 99%

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Tao

Auguste

2017

Biomaterials

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“…technique that intensi es bioactive extraction from raw materials [17]. However, this method is rather complicated, so it is necessary to gure out the interaction between independent variables during UMAT extraction.…”

Section: Response Surface and Contour Plots Umat Is A Typicalmentioning

confidence: 99%

Effects of Ultrasonic-Microwave-Assisted Technology on Hordein Extraction from Barley and Optimization of Process Parameters Using Response Surface Methodology

Guan

Liu

et al. 2018

Journal of Food Quality

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We investigated the process intensification of ultrasonic-microwave-assisted technology for hordein extraction from barley. Response surface methodology was utilized to optimize the extraction conditions and to analyze the interaction between four selected variables: temperature, microwave power, ultrasonic power, and extraction time. The validated extraction yield of hordein reached 8.84% at 78°C, microwave power 298 W, and ultrasonic power 690 W after 20 min as optimum conditions. Compared with traditional water-bath extraction (4.7%), the ultrasonic-microwave-assisted technology effectively increased the hordein extraction yield and shortened the extraction time. According to the obtained quadratic model (R2 = 0.9457), ultrasonic power and extraction time were the first two significant factors. However, temperature limited the effects of other factors during extraction. SDS-PAGE and scanning electron microscopy were used to identify the hordein extract and to clarify the difference between the two hordein fractions extracted with new and traditional methods, respectively. Ultrasonic-microwave-assisted technology provided a new way to improve hordein extraction yield from barley and could be a good candidate for industrial application of process intensification.

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“…Diversos estudos demonstram que a utilização de algumas técnicas de pré tratamento atuam como coadjuvantes no processo de extração, potencializando--o. Umas dessas técnicas é a utilização do ultrassom, que pode elevar em mais de 50% o teor de lipídios extraídos da biomassa (Dos Santos, Moreira, Kunigami, Aranda, & Teixeira, 2015;Garoma & Janda, 2016).…”

Section: Revisão Bibliográficaunclassified

Avaliação Dos Fatores Envolvidos Na Extração De Lipídios Da Biomassa Da Microalga Chlorella Minutíssima via Solventes

Zorn

Pedro

Amaral

et al. 2017

HOLOS

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As microalgas têm se tornado uma fonte promissora de energia renovável; além de fornecerem lipídios, carboidratos, proteínas, vitaminas e pigmentos, dentre outras diversas substâncias de alto valor agregado, contribuem enormemente para a redução do efeito estufa na atmosfera, ao consumirem o dióxido de carbono pela realização de fotossíntese. Os lipídios representam uma importante fonte de matéria prima para a obtenção de biocombustíveis, tal como o biodiesel. Um dos processos de obtenção de lipídios de excelente custo benefício é a extração via solventes, utilizando clorofórmio e metanol. Visando a obtenção do maior rendimento em lipídios por grama de biomassa, foram extraídos lipídios da biomassa da microalga marinha Chlorella minutíssima, empregando um arranjo ortogonal de Taguchi com o estudo simultâneo de quatro fatores que influenciam neste processo: umidade da biomassa, proporção entre os solventes, quantidade total de solventes utilizada e tempo de ultrassom para potencializar a quebra da parede celular, facilitando a liberação destes lipídios. Obteve-se um teor de 20% em lipídios, pelo emprego de uma biomassa com 64% de umidade, uma proporção de clorofórmio: metanol: água de 5,7: 3: 1, com um total de 33 mL de solventes e em apenas 70 minutos de ultrassom. Os lipídios extraídos foram transesterificados em biodiesel etílico via catálise ácida, obtendo-se uma conversão de 94,45%.

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Investigation of the effects of microalgal cell concentration and electroporation, microwave and ultrasonication on lipid extraction efficiency

Cited by 54 publications

References 29 publications

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Pattern-based sensing of triple negative breast cancer cells with dual-ligand cofunctionalized gold nanoclusters

Effects of Ultrasonic-Microwave-Assisted Technology on Hordein Extraction from Barley and Optimization of Process Parameters Using Response Surface Methodology

Avaliação Dos Fatores Envolvidos Na Extração De Lipídios Da Biomassa Da Microalga Chlorella Minutíssima via Solventes

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