Investigation of the effects of N-Acetylglucosamine on the stability of the spike protein in SARS-CoV-2 by molecular dynamics simulations

Tekin, E. Deniz

doi:10.1016/j.comptc.2023.114049

Cited by 2 publications

(3 citation statements)

References 119 publications

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“…N-linked glycans are accessory carbohydrates hanging on the S trimer surface, which are important for appropriate folding and viral entry 28 , 29 . In the present study, 12 N-linked glycans were demonstrated in the parental strain, PED-JPFP0-PJ, as well as in P0F1, P0F3, P2F1, P2F3 and P5F1.…”

Section: Discussionmentioning

confidence: 99%

Evolution and virulence of porcine epidemic diarrhea virus following in vitro and in vivo propagation

Jermsutjarit,

Mebumroong,

Watcharavongtip

et al. 2024

Sci Rep

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Practice of inoculating porcine epidemic diarrhea virus (PEDV) in piglets generating feedback material might influence the genetic evolution and attenuation of PEDV. The study was conducted to evaluate evolutionary rate and attenuation following serial in vitro and in vivo propagation. In the study, PED-JPFP0-PJ, Passage 0 (P0), was isolated from infected pigs and serially passaged in Vero cells for 5 consecutive times, P1-P5. P0, P2 and P5 were then subjected to orally inoculate 3-day-old piglets. At 24 h post inoculation, intestines of each passage (F1), were collected, and subsequently sub-passaged in piglets for 2 additional passages (F2-F3). Virus titration, PEDV genomic copies number, VH:CD ratios, and immunohistochemistry were evaluated. S and ORF3 genes were characterized. The results of the study demonstrated that virus titer and virulence were negatively correlated with increased passages, both in vitro and in vivo. Increased substitution rate was observed in higher passages. The evolutionary rate of S gene was higher than that of ORF3. Seven aa changes at positions 223, 291, 317, 607, 694, 1114 and 1199, with reduced N-linked glycan were observed in P5F3. In conclusion, serial passage of PEDV, both in vitro and in vivo, influence the genetic development and the attenuation of PEDV.

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Section: Discussionmentioning

confidence: 99%

Evolution and virulence of porcine epidemic diarrhea virus following in vitro and in vivo propagation

Jermsutjarit,

Mebumroong,

Watcharavongtip

et al. 2024

Sci Rep

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show abstract

“…The size of simulated box containing SIRPα V1 and V2 was 10 × 10 × 13.5 nm 3 and 10 × 11.5 × 14 nm 3 , respectively. The three parallel simulations of binary and ternary systems were performed by changing the initial motion direction, which was also widely used. ,, The interactions of the inhibitors, CD47 and SIRPα (V1 and V2, respectively), were studied.…”

Section: Methodsmentioning

confidence: 99%

“…The three parallel simulations of binary and ternary systems were performed by changing the initial motion direction, which was also widely used. 46,50,51 The interactions of the inhibitors, CD47 and SIRPα (V1 and V2, respectively), were studied.…”

Section: ■ Introductionmentioning

confidence: 99%

Biomimetic Design of Peptide Inhibitor to Block CD47/SIRPα Interactions

Zheng,

Ji,

et al. 2023

Langmuir

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Investigation of the effects of N-Acetylglucosamine on the stability of the spike protein in SARS-CoV-2 by molecular dynamics simulations

Cited by 2 publications

References 119 publications

Evolution and virulence of porcine epidemic diarrhea virus following in vitro and in vivo propagation

Evolution and virulence of porcine epidemic diarrhea virus following in vitro and in vivo propagation

Biomimetic Design of Peptide Inhibitor to Block CD47/SIRPα Interactions

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