Investigation of the effects of alkane phosphonic acid/RGD coatings on cell spreading and the interfacial strength between human osteosarcoma cells and Ti–6Al–4V

Bly, Randy; Cao, Yifang; Moore, W. A.; Soboyejo, W. O.

doi:10.1016/j.msec.2006.02.005

Cited by 12 publications

(3 citation statements)

References 18 publications

(30 reference statements)

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“…Although these silicate‐based materials are not comparable with bone implant materials such as Ti, the described carbodiimide approach can also be of interest for the attachment of ALP onto bone implant surfaces. In addition to the immobilization of entire proteins, numerous studies have been dedicated to the deposition of peptide sequences onto implant surface such as RGD146–150 and GFOGER 151–153. Compared with entire proteins, peptide sequences can be synthesized straightforward via solid phase peptide synthesis approaches.…”

Section: Deposition Of Biomoleculesmentioning

confidence: 99%

Wet‐Chemical Deposition of Functional Coatings for Bone Implantology

Nijhuis

Leeuwenburgh

Jansen

2010

Macromolecular Bioscience

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Since the early 1980s, research on the modification of bone implant surfaces by applying coatings has mainly focused on the application of inorganic calcium phosphate (CaP) coatings using physical deposition techniques. Organic components of the extracellular bone matrix, on the other hand, play an essential role in the process of bone healing, but cannot be applied using these physical techniques. Therefore, a recent trend in biomaterials research involves development of novel wet-chemical deposition techniques for both inorganic and organic coatings. This study reviews the major wet-chemical coating techniques that are used for the deposition of inorganic CaP coatings and organic biomolecules coatings.

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Section: Deposition Of Biomoleculesmentioning

confidence: 99%

Wet‐Chemical Deposition of Functional Coatings for Bone Implantology

Nijhuis

Leeuwenburgh

Jansen

2010

Macromolecular Bioscience

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“…The above results suggest that the engineering of interfaces between biomedical implants and cells/tissue may be accomplished by the introduction of ECM coatings onto their surfaces [21,22]. This has been shown in prior work [22], to improve the initial adhesion between biomedical surfaces and biological cells/tissue, during the first few days that it takes to secrete and transport the ECM layer to the regions between the cell membrane and the substrate.…”

Section: Discussionmentioning

confidence: 57%

“…Furthermore, due to biomedical importance of the adhesive interactions between cells and surfaces, a number of researchers [8][9][16][17][18][19][20][21][22] have developed different techniques to measure the strength of cell adhesion to surfaces, which are useful in characterizing cell interactions with biomaterials and also to estimate cell detachment forces.…”

Section: Introductionmentioning

confidence: 99%

Single Cell Deformation and Detachment Models of Shear Assay Measurements

Ani

Danyuo

Odunsoya³

et al. 2015

AMR

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This paper presents concepts for the modeling of cell deformation and cell detachment from biocompatible biomedical materials. A combination of fluid mechanics and fracture mechanics concepts is used to model the detachment of cells under shear assay conditions. The analytical and computational models are validated by shear assay experiments in which human-osteo-sarcoma (HOS) cell are detached from surfaces that are relevant to bio-micro-electro-mechanical systems (BioMEMS), bio-microelectronics and orthopaedic/dental implants. The experiments revealed that cell detachment occurs from patches in which of α/β integrins are separated from the extracellular matrix that is left on the substrate. The stress/strain distribution and energy release rates associated with the observed detachments are also computed using elastic cell deformation, fluid/structure interactions and linear fracture mechanics (LEFM) model. The simulations reveal show that cancer cells generally experience higher levels of deformation than normal cells. The simulations also revealed that the cell-extracellular matrix interface was prone to cell detachment (interfacial failure), as observed in the shear assay experiments. The critical energy release rates for normal cell detachment were also found to be greater than those required for the detachment of cancer cells. The implications of the results are discussed for the design of biomedical implants and their interfaces.

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