Investigation of the Effects of Some Phenolic Compounds on the Activities of Glucose‐6‐Phosphate Dehydrogenase and 6‐Phosphogluconate Dehydrogenase from Human Erythrocytes

Abstract

Polyphenols are the important compounds that have various bioactivities. They constitute vital active agents of not only daily diet but also natural medicines that are used traditionally. It is generally considered that they are safe because they are natural. In some conducted studies, different negative effects of these compounds were mentioned. Twelve phenolic compounds have been assayed to determine the effect of inhibition on glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) enzymes activity. For in vitro studies, the enzymes were purified from human erythrocytes using 2',5'-ADP Sepharose 4B affinity chromatography. Naringenin, caffeic acid, ellagic acid, ferulic acid, and sinapic acid against two enzymes, hesperidin and polydatin, only on G6PD activity and chrysin solely against 6PGD showed inhibitory effect. Chlorogenic acid, p-coumaric acid, and syringic acid did not exhibit an effect on the activity of the two enzymes.

Compounds from <em>Terminalia mantaly</em> L. (Combretaceae) Stem Bark Exhibit Potent Inhibition against some Pathogenic Yeasts and Enzymes of Metabolic Significance<strong></strong>

“…In this study, the substituted ellagic acid derived compound 1 did not show any effect on the G6PD enzyme activity, although Adem et al [45] reported that ellagic acid inhibited the Previous studies by Sarıkaya et al [46] have indicated that ellagic acid inhibited hCA I and hCA II with Ki values of 0.207 and 0.146 mM respectively. In the present study, compound 1, a substituted derivative of ellagic acid (1a) has exhibited moderate, however highly improved potency toward hCA I (Ki= 44.11 µM) and hCA II (55.78 µM) enzymes.…”

“…In contrast, G6PD-deficient tumor cell lines showed relatively slow growth and enhanced apoptosis [41]. Previous studies also reported G6PD inhibitory properties for few compounds such as steroids and derivatives [42,43], chalcones [28], catechin gallates [44], and some phenolic molecules [45].…”

Purification and biochemical characterization of glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase and glutathione reductase from rat lung and inhibition effects of some antibiotics

“…They have been purified from several mammalian tissues with various techniques such as rat liver and kidney [8][9][10] , human erythrocyte 11,12 , rat small intestine 13 , grass carp hepatopancreases 14 , rainbow trout liver 15 and lamb kidney 16 , and some of their characteristics and kinetic properties have been determined.…”

Impact of Diabetes Mellitus Type 2 in the Activity of Glucose-6-Phosphate Dehydrogenase in Human Erythrocyte

“…The housekeeping enzymes are G6PD, GSH reductase, and 6-phosphogluconate dehydrogenase, which are found in animal tissues, plants, microorganisms, and blood cells [7]. The different techniques will be used, and they can be isolated from various sources, such as the kidneys and liver of rats [8,9,12], human erythrocytes [10], liver of rainbow trout [11], [12]. The kinetic properties and characteristics of the enzymes have been assessed.…”