Investigation of the Effects of the Endogenous Cannabinoid Anandamide on Luminal A Breast Cancer Cell Line MCF-7

Abstract

The present study was carried out to investigate anti-tumoral effects of Anandamide (AEA) in luminal A breast cancer cell line MCF-7. Cell viability was measured by MTT assay and cell index was measured by xCelligence DP analyzer system. The Feulgen method was used to determine the mitotic index parameter, and the 3 H-Thymidine method was used to determine the labeling index parameter. The apoptotic index parameter was determined using a fluorescent dye DAPI. The results of this study showed that 25 µM Anandamide concentration was the optimum concentration for MCF-7 cells. While this concentration decreased the proportion of cells in the mitotic phase and synthesis phase, it increased the proportion of apoptotic cells.

The Interaction of the Endocannabinoid Anandamide and Paracannabinoid Lysophosphatidylinositol during Cell Death Induction in Human Breast Cancer Cells

“…In all cell lines tested, AEA had either a cytotoxic effect or a mixture of cytotoxic and anti-proliferative ones. This observation was in line with the already published data [39,40], and an anti-proliferative effect was also observed at low concentrations of the substance [41]. Our research, however, extends the known data to several cell lines, namely BT-20, MCF-10A, and SK-BR-3, and adds data on the activity of selective CB1 receptor agonist ACEA in these models.…”