Investigation of the Effect of the Degree of Processing of Radix Rehmanniae Preparata (Shu Dihuang) on Shu Dihuangtan Carbonization Preparation Technology

Abstract

Carbonization of Radix Rehmanniae Preparata (Shu Dihuangtan) via stir-frying could increase its homeostasis maintaining and antidiarrheal effects. To ensure these pharmacological functions, the quality of the raw material (processed Rehmanniae Radix) must be well controlled. Therefore, we analyzed the effects of different degrees of processing and adjuvants on processed Rehmanniae Radix (Shu Dihuang) by High Performance Liquid Chromatography (HPLC) chromatographic fingerprints, thermal gravimetric analysis and Fourier transform infrared spectroscopy (FTIR). Based on the results from HPLC fingerprints combined with similarity analysis (SA) and hierarchical cluster analysis (HCA) the optimum processing method for Shu Dihuang was five cycles of steaming and polishing, which follows the ancient processing theory. The intensity of thermal weight loss rate peaked near 210.33 ± 4.32 °C or 211.33 ± 2.62 °C, which was an important indicator for the degree of processing of Shu Dihuang. A temperature near 290.89 ± 2.51 °C was the upper limit for carbonizing Shu Dihuangtan. FTIR spectroscopy analysis showed that the overall chemical composition of Shu Dihuangtan was affected by both the degree of processing and adjuvant, which are very important for its quality.

Application of delayed luminescence method on measuring of the processing of Chinese herbal materials

“…It indicates that the DL characteristics became stable after the fourth processing cycle. This result is similar with previous chemical analyses which indicated that the chemical components were stable after the fifth processing cycle using plant metabolomics tools such as 1 HNMR and gas chromatography–mass spectrometry (GC–MS) [ 4 , 25 , 26 ]. Plant metabolomics provides a relatively comprehensive analysis of various compounds in biological systems based on specific platforms [ 30 ], and it has been considered as a suitable tool for herb quality control in a system level [ 31 ].…”

“…Previous studies suggested that differences in chemical profiles of processed Rehmanniae radix became smaller after five processing cycles (Additional file 2 : Fig. S1) [ 4 , 25 , 26 ]. In this study, DL has been used to measure raw and processed Rehmanniae radix samples in order to investigate the processing approach.…”

“…Thus, DL shows similarities in identified effects to indicate the processing of Rehmanniae radix compared to chemical analyses (Additional file 2 : Fig. S1) [ 4 , 25 , 26 ].

Fig.

…”

“…The data presented in this article shows that DL properties from raw and processed herbal materials can be distinguished significantly. DL measurements show similar results to differentiate raw and processed Rehmanniae radix compared to chemical analysis using plant metabolomics [ 4 , 25 , 26 ]. The results indicate that after four cycles of processing for Rehmanniae radix, there is no much significant change in DL parameters which resembles the results obtained from chemical analysis (after five cycles).…”

<p>Traditional Chinese Medicine Jiuwei Zhenxin Granules in Treating Depression: An Overview</p>

“…The chemical composition of RG is dominated by glycosides, mainly cyclic allene terpenoids, including leonuride, aucubin, catalpol, and rehmannioside A, B, C, and D; sugars, including D-glucose, D-galactose, D-fructose, and sucrose; and a variety of amino acids and inorganic elements. [105][106][107] RG has a wide range of clinical applications. It is currently reported that RG can be used to treat attention deficit hyperactivity disorder, 108 PD 109 , multiple sclerosis, 110 anxiety, 111 and memory disorders, 112 and it inhibits inflammatory responses 113,114 and has anti-fatigue activity.…”