Investigation of the effect of hyperforin and hypericin on inflammatory response in RAW 264.7 macrophages

Berköz, Mehmet; Allahverdiyev, Oruç; Yıldırım, Metin

doi:10.5505/vtd.2018.07769

Cited by 7 publications

(3 citation statements)

References 18 publications

(23 reference statements)

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“…Also, Lee et al investigated the anti‐inflammatory effects of hyperforin and hypericin in lipopolysaccharide‐stimulated RAW macrophages and found that these compounds reduced iNOS and cyclooxygenase‐2 (COX‐2) gene expression in a dose‐dependent pattern (Lee et al, 2016). Berköz et al reported that hyperforin and hypericin reduced NO and PGE2 production, as well as a dosage‐dependent pattern of iNOS and COX‐2 gene expression in RAW 264.7 macrophages (Berköz et al, 2018) hence inflammation decreased (de Villiers et al, 2014).…”

Section: Wound Healing Properties Of H Perforatum L and Associated Me...mentioning

confidence: 99%

The potential role of Hypericum perforatum in wound healing: A literature review on the phytochemicals, pharmacological approaches, and mechanistic perspectives

Farasati Far,

Gouranmohit,

Naimi‐jamal

et al. 2024

Phytotherapy Research

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St. John's Wort, commonly known as Hypericum perforatum L., is a flowering plant in the Clusiaceae family that traditionally been employed for treating anxiety, depression, wounds, burns, sunburn, irritation, and stomach ailments. This review provides a synopsis of H. perforatum L. phytoconstituents and their biological effects, highlighting its beneficial therapeutic properties for dermatological indications, as well as its antioxidant, antimicrobial, anti‐inflammatory, and anti‐angiogenic activity in various applications including wound healing and skin conditions such as eczema, sun burn and minor burns also spastic paralysis, stiff neck and mood disorders as anti‐depressant and nerve pains such as neuralgia. The data were collected from several databases as Web of Science PubMed, ScienceDirect, Scopus and Google Scholar using the terms: “H. perforatum L.”, “H. perforatum L. /phytochemistry,” and “H. perforatum extracts/wound healing” collected from 1994 to 2023. The findings suggest H. perforatum L. acts through various mechanisms and plays a role in each phase of the wound healing process, including re‐epithelialization, angiogenesis, wound contraction, and connective tissue regeneration. H. perforatum L. enhances collagen deposition, decreases inflammation, inhibits fibroblast migration, and promotes epithelialization by increasing the number of fibroblasts with polygonal shape and the number of collagen fibers within fibroblasts. H. Perforatum L. extracts modulate the immune response and reduce inflammation were found to accelerate the wound healing process via inhibition of inflammatory mediators' production like interleukin‐6, tumor necrosis factor‐α, cyclooxygenase‐2 gene expression, and inducible nitric oxide synthase. Thus, H. perforatum L. represents a potential remedy for a wide range of dermatological problems, owing to its constituents with beneficial therapeutic properties. H. perforatum L. could be utilized in the development of novel wound healing therapies.

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Section: Wound Healing Properties Of H Perforatum L and Associated Me...mentioning

confidence: 99%

The potential role of Hypericum perforatum in wound healing: A literature review on the phytochemicals, pharmacological approaches, and mechanistic perspectives

Farasati Far,

Gouranmohit,

Naimi‐jamal

et al. 2024

Phytotherapy Research

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“…On the other hand, hypericin and hyperforin have been shown to reduce inflammatory prostaglandin E2 and COX-2 (cyclooxygenase-2) release from activated macrophages. It is reported that hypericin and hyperforin can be potential target drugs in cancer treatment due to their anti-inflammatory effect 9 .…”

Section: Introductionmentioning

confidence: 99%

Apoptotic and Anti-inflammatory Effects of Hypericum Perforatum Extract in Human Basal Cell Carcinoma TE 354.T Cell Line

Çelik

2021

Dicle Tıp Dergisi

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Objectives: Basal cell carcinoma is the most common malignancy in in the Caucasian race and is a serious health problem with its ever-increasing incidence and high cost. Although the treatment is surgical, some elderly patients do not accept surgical intervention. In this case, non-surgical treatments come into prominence. The Hypericum perforatum (HP) / St. John's Wort plant has been used in the treatment of many diseases for centuries among local people. Here, we aimed to aimed to elucidate the effect and molecular mechanism of the HP plant on BCC cells. Methods: Human BCC cell lines TE 354.T were acquired from ATCC®, then were cultured in Dulbecco's Modified Eagle's Medium. BCC cells were seeded at a concentration of 2x106cells in each flask (n=12). After incubation, while six of the flasks were applied with a 0.8% HP extract and 99.2% medium for 48 hours, no application was made to the control group. The effects of HP on mitochondrial mediated intrinsic and extrinsic apoptotic pathway agents, cell cycle G2 / M checkpoint kinases and inflammatory mediators were investigated in BCC cancer cells. The expression and activities of these mediators in both groups were evaluated by ELISA test. Results: It was determined that HP extract treatment increased the expression of apoptotic proteins (AIF, GADD153, GRP78, caspase-3, Bax) and cell cycle G2 / M checkpoint kinase (Wee1), though it reduced antiapoptotic protein (Bcl-2) and inflammatory mediators (iNOS, COX-2, cPLA2, NFkB) in BCC cell lines. Conclusion: HP extract can support routine chemotherapy on BCC cells with its apoptotic, anti-inflammatory and anti-cancer effect, and may be a beacon of hope in reducing resistance to chemotherapeutic drugs in the treatment of BCC.

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“…It has been demonstrated that hyperforin suppressed the activities of 5-lipoxygenase and cyclooxygenase (COX) enzymes which play a pivotal roles in regulation of inflammatory and immune responses (Albert et al, 2002). In another study on the antiinflammatory effects of hypericin and hyperforin, it has been reported that these substrates showed anti-inflammatory activity by decreasing prostaglandin E2 (PGE2) and nitric oxide (NO) production and COX-2 and inducible nitric oxide synthase (iNOS) gene expression (Berköz et al, 2018). HP has been reported to be effective antioxidant, antitumor and antimicrobial activities due to its flavonoid and some other phenolic components (Cakir et al, 2003;Milosevic et al, 2007;Pia Schiavone et al, 2014;Tungmunnithum et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Investigation of Apoptotic Effects of Hypericum perforatum Extract on Breast Cancer Cell Line

Alp

Tutun

Kaplan

et al. 2019

Harran Üniversitesi Veteriner Fakültesi Dergisi

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Hypericum perforatum has biological active contents affecting a variety of proteins such as caspase-3, bcl-2, and bax, which mediate apoptosis known as programmed cell death and exerting anti-inflammatory effect. Apoptotic pathways are important for cancers, chemotherapeutic resistance, and cancer development. Anti-inflammatory agents are also a potential target for cancer. Therefore, it was aimed to investigate the activity of caspase-3 and the expressions of bcl-2, bax, wee 1, gadd153, grp78, AIF, iNOS, COX-2, cPLA2, and NF-κB in H. perforatum extract-treated breast cancer (BC) cells in this study. The activity of caspase-3 and the expressions of these proteins were determined in the cells by ELISA. The HP extract increased the activity of caspase-3 and the expressions of bax, wee 1, gadd153, grp78 and AIF, and decreased the expressions of bcl-2, COX-2, iNOS, cPLA2 and NF-κB in the BC cells. In the light of these findings, HP extract could help to inhibit grow of BC cells and its anti-inflammatory effect may contribute this effect.

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Investigation of the effect of hyperforin and hypericin on inflammatory response in RAW 264.7 macrophages

Abstract: Acute inflammatory response represents an initial protective mechanism in the body (1,2). However,

Cited by 7 publications

References 18 publications

The potential role of Hypericum perforatum in wound healing: A literature review on the phytochemicals, pharmacological approaches, and mechanistic perspectives

The potential role of Hypericum perforatum in wound healing: A literature review on the phytochemicals, pharmacological approaches, and mechanistic perspectives

Apoptotic and Anti-inflammatory Effects of Hypericum Perforatum Extract in Human Basal Cell Carcinoma TE 354.T Cell Line

Investigation of Apoptotic Effects of Hypericum perforatum Extract on Breast Cancer Cell Line

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