Investigation of the Cytotoxic Effects of Titanate Nanotubes on Caco-2 Cells

Fenyvesi, Ferenc; Rázga, Zsolt; Vecsernyés, Miklós; Kása, P.; Pintye‐Hódi, Klára; Bácskay, Ildikó

doi:10.1208/s12249-014-0115-x

Cited by 19 publications

(17 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cytotoxicity of titanate nanotubes made by hydrothermal treatment has been assessed in H596 human lung tumor cells [30], cardiomyocytes [31], SNB19 and U87-MG glioblastomas [32], Caco-2 cells [33], as well as 22Rv1 prostate cancer cells [8]. Interestingly, the degree of ion exchange via acid treatment, which partly or entirely substitutes sodium cations by hydrogen cations, is a key parameter that drives the cytotoxicity of titanate nanowires [30].…”

Section: Cytotoxicity Of Tionts: the Surface Chemistry Mattersmentioning

confidence: 99%

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Maurizi¹,

Papa²,

Boudon³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

View full text Add to dashboard Cite

The lack of toxicological data on nanomaterials makes it difficult to assess the risk related to their exposure, and as a result further investigation is required. This chapter presents the synthesis of controlled oxide nanoparticles followed by the evaluation of their safety profile or toxicity (iron, titanium and zinc oxides). The controlled surface chemistry, dispersion in several media, morphology and surface charge of these nanoparticles are presented (transmission electron microscopy, dynamic light scattering, zeta potential, X-ray photoelectron spectroscopy). Classical cytotoxic and cellular uptake studies on different cancer cell lines from liver, prostate, heart, brain and spinal cord are discussed. The incidence of nanoparticles on biogenesis and activity of cell organelles is also highlighted, as well as their biodistribution in animal models. The acute toxicity on zebrafish embryo model is also presented. Finally, the stress is put on the influence and the necessity of controlling the protein corona, a layer of plasma proteins physically adsorbed at the surface of such nanoparticles as a result of their presence in the bloodstream (or relevant biological fluids).

show abstract

Section: Cytotoxicity Of Tionts: the Surface Chemistry Mattersmentioning

confidence: 99%

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Maurizi¹,

Papa²,

Boudon³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

View full text Add to dashboard Cite

show abstract

“…Similarly, TiONts are widely studied [29][30][31] in a broad range of applications since their discovery in the late 1990s [32]. Recently, TiONt applications have been developed in several fields of biomedicine [29,33], such as orthopedics and dental implants [34], dopamine detection [35], DNA transfection [36] and adsorption [37], bioimaging [38,39], safe nanocarrier [36,40,41], drug delivery (genistein and docetaxel) [42][43][44], and cancer cell radiosensitization [44,45]. These TiONt applications are possible due to the atypical morphology shared with CNTs and HNTs.…”

Section: Introductionmentioning

confidence: 99%

Titanate Nanotubes Engineered with Gold Nanoparticles and Docetaxel to Enhance Radiotherapy on Xenografted Prostate Tumors

Loiseau

Boudon

Oudot

et al. 2019

Cancers

View full text Add to dashboard Cite

Nanohybrids based on titanate nanotubes (TiONts) were developed to fight prostate cancer by intratumoral (IT) injection, and particular attention was paid to their step-by-step synthesis. TiONts were synthesized by a hydrothermal process. To develop the custom-engineered nanohybrids, the surface of TiONts was coated beforehand with a siloxane (APTES), and coupled with both dithiolated diethylenetriaminepentaacetic acid-modified gold nanoparticles (Au@DTDTPA NPs) and a heterobifunctional polymer (PEG3000) to significantly improve suspension stability and biocompatibility of TiONts for targeted biomedical applications. The pre-functionalized surface of this scaffold had reactive sites to graft therapeutic agents, such as docetaxel (DTX). This novel combination, aimed at retaining the AuNPs inside the tumor via TiONts, was able to enhance the radiation effect. Nanohybrids have been extensively characterized and were detectable by SPECT/CT imaging through grafted Au@DTDTPA NPs, radiolabeled with 111In. In vitro results showed that TiONts-AuNPs-PEG3000-DTX had a substantial cytotoxic activity on human PC-3 prostate adenocarcinoma cells, unlike initial nanohybrids without DTX (Au@DTDTPA NPs and TiONts-AuNPs-PEG3000). Biodistribution studies demonstrated that these novel nanocarriers, consisting of AuNP- and DTX-grafted TiONts, were retained within the tumor for at least 20 days on mice PC-3 xenografted tumors after IT injection, delaying tumor growth upon irradiation.

show abstract

“…In this regard, anodized TNTs, which are surface attached carriers, may beneficially be used in implantology [13,14,15], while hydrothermally synthesised TNTs, which are separate carriers, can be loaded and/or functionalized with active substances [16,17] and provide a large spectra of drug delivery approaches. Concentrating on hydrothermally synthesised TNTs, it is important to underline that these nano-materials are completely biocompatible and assure safe use without any toxicity risks, as discussed in several studies [18,19]. This property makes them much more valuable for nanomedicine than many other nano-materials, e.g., carbon nanotubes, and necessitates the detailed pharmaceutical investigation of TNTs.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Compressibility and Compactibility of Titanate Nanotube-API Composites

et al. 2018

Self Cite

View full text Add to dashboard Cite

The present work aims to reveal the pharma-industrial benefits of the use of hydrothermally synthesised titanate nanotube (TNT) carriers in the manufacturing of nano-sized active pharmaceutical ingredients (APIs). Based on this purpose, the compressibility and compactibility of various APIs (diltiazem hydrochloride, diclofenac sodium, atenolol and hydrochlorothiazide) and their 1:1 composites formed with TNTs were investigated in a comparative study, using a Lloyd 6000R uniaxial press instrumented with a force gauge and a linear variable differential transformer extensometer. The tablet compression was performed without the use of any excipients, thus providing the precise energetic characterisation of the materials’ behaviour under pressure. In addition to the powder functionality test, the post-compressional properties of the tablets were also determined and evaluated. The results of the energetic analysis demonstrated that the use of TNTs as drug carriers is beneficial in every step of the tabletting process: besides providing better flowability and more favourable particle rearrangement, it highly decreases the elastic recovery of the APIs and results in ideal plastic deformation. Moreover, the post-compressional properties of the TNT–API composites were found to be exceptional (e.g., great tablet hardness and tensile strength), affirming the above results and proving the potential in the use of TNT carriers for drug manufacturing.

show abstract

Investigation of the Cytotoxic Effects of Titanate Nanotubes on Caco-2 Cells

Cited by 19 publications

References 15 publications

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Toxicological Risk Assessment of Emerging Nanomaterials: Cytotoxicity, Cellular Uptake, Effects on Biogenesis and Cell Organelle Activity, Acute Toxicity and Biodistribution of Oxide Nanoparticles

Titanate Nanotubes Engineered with Gold Nanoparticles and Docetaxel to Enhance Radiotherapy on Xenografted Prostate Tumors

Investigation of the Compressibility and Compactibility of Titanate Nanotube-API Composites

Contact Info

Product

Resources

About