Investigation of the colour reaction of phenols with 4-aminoantipyrine

Svobodová, D.; Gasparič, J.

doi:10.1007/bf01219640

Cited by 32 publications

(15 citation statements)

References 10 publications

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“…The assay has a greater sensitivity than other colorimetric assays of phenol (Svobodová and Gasparič, 1971) and is most sensitive in the range of 0.2–2.0 µg ml −1 . The colour develops within 2 min, and changes with time, but it does not significantly change within 1 h (Martin, 1949; Svobodová and Gasparič, 1971; Gasparic et al ., 1974). The reagents and phenol standards were prepared just prior to their use.…”

Section: Methodsmentioning

confidence: 99%

Fourier transform infrared spectroscopy as a metabolite fingerprinting tool for monitoring the phenotypic changes in complex bacterial communities capable of degrading phenol

Wharfe

Jarvis²,

Winder

et al. 2010

Environmental Microbiology

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The coking process produces great volumes of wastewater contaminated with pollutants such as cyanides, sulfides and phenolics. Chemical and physical remediation of this wastewater removes the majority of these pollutants; however, these processes do not remove phenol and thiocyanate. The removal of these compounds has been effected during bioremediation with activated sludge containing a complex microbial community. In this investigation we acquired activated sludge from an industrial bioreactor capable of degrading phenol. The sludge was incubated in our laboratory and monitored for its ability to degrade phenol over a 48 h period. Multiple samples were taken across the time-course and analysed by Fourier transform infrared (FT-IR) spectroscopy. FT-IR was used as a whole-organism fingerprinting approach to monitor biochemical changes in the bacterial cells during the degradation of phenol. We also investigated the ability of the activated sludge to degrade phenol following extended periods (2-131 days) of storage in the absence of phenol. A reduction was observed in the ability of the microbial community to degrade phenol and this was accompanied by a detectable biochemical change in the FT-IR fingerprint related to cellular phenotype of the microbial community. In the absence of phenol a decrease in thiocyanate vibrations was observed, reflecting the ability of these communities to degrade this substrate. Actively degrading communities showed an additional new band in their FT-IR spectra that could be attributed to phenol degradation products from the ortho- and meta-cleavage of the aromatic ring. This study demonstrates that FT-IR spectroscopy when combined with chemometric analysis is a very powerful high throughput screening approach for assessing the metabolic capability of complex microbial communities.

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Section: Methodsmentioning

confidence: 99%

Fourier transform infrared spectroscopy as a metabolite fingerprinting tool for monitoring the phenotypic changes in complex bacterial communities capable of degrading phenol

Wharfe

Jarvis²,

Winder

et al. 2010

Environmental Microbiology

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“…As it shown in Effect of pH and 4-AAP and potassium hexacyanoferrate The extraction step was followed by reaction with 4-AAP in the presence of hexacyanoferrate at room temperature [26]. As it shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultrasound assisted cold-induced aggregation: an improved method for trace determination of volatile phenol

et al. 2012

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We report on a simple, rapid and efficient extraction procedure, referred to as ultrasound-assisted cold-induced aggregation (USA-CIAME), for the extraction of phenol from aqueous samples. In this method, very small amounts of the ionic liquid 1-hexyl-3-methylimidazolium hexafluorophosphate (the extractant) are dissolved in a sample solution containing phenol and ultrasonicated for 1 min. The solution is cooled in an ice bath upon which a cloudy solution forms. Following centrifugation, the extractant phase settles at the bottom of a conical-bottom centrifuge tube. Phenol is photometrically determined after its chromogenic reaction with 4-aminoantipyrine in the presences of hexacyanoferrate at pH 10.0. Compared to the conventional cold-induced aggregation microextraction (CIAME) and dispersive liquid liquid microextraction (DLLME), the optimized approach displays the highest extraction efficiency at room temperature, and the shortest extraction time (5 min). Key parameters affecting the performance were evaluated and optimized. Under optimum conditions, the limit of detection of phenol is 0.86 μg L −1 , and the enrichment factor is 75. The calibration graph as linear over the range from 3 to 150 μg L −1 , and the relative standard deviation is 2.65% (n05). The method was successfully applied to the determination of phenol in water samples.

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“…Thus, the sequence (1) D-B-AAP-HCF gave higher values of absorbance. When mixing AAP and HCF (sequence 2), a red colour solution is obtained before adding D and B, probably due to the generation of antipyrine red [12,13]. The sequence (3) B-D-HCF-AAP gave an absorbance less by half than in the case of sequence (1).…”

Section: Order Of Addition Of Reactantsmentioning

confidence: 99%

“…An important factor that must be taken into account involves the concentration ratio of AAP and the oxidizing agent [12,13]. At a constant concentration of AAP (2.5 10 -3 mol L -1 ), the concentration of HCF was varied in the following range: 2.5 10 -3 -5.5 10 -3 mol L -1 As is shown in Fig.…”

Section: Effect Of Aap and Hcf Concentrationmentioning

confidence: 99%

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A Spectrophotometric Method for Diosmin Determination

Moldovan¹,

Bunaciu²,

Al-Omar³

et al. 2010

TOCBMJ

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A rapid and sensitive spectrophotometric method for the determination of diosmin (D) has been developed. The method is based on the reaction of D with 4-aminoantipyrine (AAP) in the presence of hexacyanoferrate (III) (HCF) in an alkaline medium and subsequent formation of a purple coloured product having max at 524.5 nm. The conditions affecting the reaction (reagents concentration, pH, order of addition of reagents, stability in time) were optimized. Under the optimum conditions, Beer's law was obeyed in the range of 0.3 -35 μg mL -1 D with a correlation coefficient of 0.9993. The assay limits of detection and quantitation were 0.1 and 0.3 μg mL -1 , respectively. The proposed method was successfully applied to the analysis of the drug tablets formulation. The results were compared favorably with those obtained by other reported methods.

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Investigation of the colour reaction of phenols with 4-aminoantipyrine

Cited by 32 publications

References 10 publications

Fourier transform infrared spectroscopy as a metabolite fingerprinting tool for monitoring the phenotypic changes in complex bacterial communities capable of degrading phenol

Fourier transform infrared spectroscopy as a metabolite fingerprinting tool for monitoring the phenotypic changes in complex bacterial communities capable of degrading phenol

Ultrasound assisted cold-induced aggregation: an improved method for trace determination of volatile phenol

A Spectrophotometric Method for Diosmin Determination

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