Investigation of the bystander effect in CHO-K1 cells

Kaźmierczak, Urszula; Banaś, D.; Braziewicz, J.; Buraczewska, Iwona; Czub, Joanna; Jaskóła, M.; Kaźmierczak, Ł.; Korman, A.; Kruszewski, Marcin; Lankoff, Anna; Lisowska, Halina; Nesteruk, Marta; Szefliński, Z.; Wojewódzka, Maria

doi:10.1016/j.rpor.2014.04.015

Cited by 5 publications

(3 citation statements)

References 16 publications

(16 reference statements)

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“…Ionizing radiation affects cells directly and indirectly, and can lead to an increase of the frequency of biological phenomena such as cell death, malignant transformations and chromosomal aberrations [9][10][11]. Cytogenetic biological dosimetry measures radiation-induced chromosomal aberrations as a proxy to estimate a received dose [12,13].…”

Section: Introductionmentioning

confidence: 99%

The Cytokinesis-Block Micronucleus Assay on Human Isolated Fresh and Cryopreserved Peripheral Blood Mononuclear Cells

Sioen

Cloet

Vral

et al. 2020

JPM

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The cytokinesis-block micronucleus (CBMN) assay is a standardized method used for genotoxicity studies. Conventional whole blood cultures (WBC) are often used for this assay, although the assay can also be performed on isolated peripheral blood mononuclear cell (PBMC) cultures. However, the standardization of a protocol for the PBMC CBMN assay has not been investigated extensively. The aim of this study was to optimize a reliable CBMN assay protocol for fresh and cryopreserved peripheral blood mononuclear cells (PBMCS), and to compare micronuclei (MNi) results between WBC and PBMC cultures. The G0 CBMN assay was performed on whole blood, freshly isolated, and cryopreserved PBMCS from healthy human blood samples and five radiosensitive patient samples. Cells were exposed to 220 kV X-ray in vitro doses ranging from 0.5 to 2 Gy. The optimized PBMC CBMN assay showed adequate repeatability and small inter-individual variability. MNi values were significantly higher for WBC than for fresh PBMCS. Additionally, cryopreservation of PBMCS resulted in a significant increase of MNi values, while different cryopreservation times had no significant impact. In conclusion, our standardized CBMN assay on fresh and cryopreserved PBMCS can be used for genotoxicity studies, biological dosimetry, and radiosensitivity assessment.

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Section: Introductionmentioning

confidence: 99%

The Cytokinesis-Block Micronucleus Assay on Human Isolated Fresh and Cryopreserved Peripheral Blood Mononuclear Cells

Sioen

Cloet

Vral

et al. 2020

JPM

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“…In 1992, this phenomenon was described by Nagasawa and Little, 8 although the underlying mechanisms for the bystander effect are still not fully elucidated. Interestingly, Urszula Kaźmierczak et al 9 investigated this effect in Chinese hamster ovary cells (CHO-K1) irradiated with high LET 12C ions and X-rays at the dose range of 0.1-4 Gy. As the authors report, no bystander effect was observed.…”

Section: The Next Generation Of Medical Physicistsmentioning

confidence: 99%

The next generation of medical physicists

Kukołowicz¹

2014

Reports of Practical Oncology & Radiotherapy

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“…There is considerable evidence that ionizing radiation affects cells directly or indirectly. 1 In general, these effects increase the frequency of apoptosis, micronucleation, DNA strand breaks and mutations, altered levels or activity of regulatory proteins and enzymes, reduced clonogenic efficiency, and oncogenic transformation. 2 Cytogenetic biodosimetry is a methodology based on the measurement of radiation induced biological effects visible at the cytogenetic level in order to correlate them with the dose of radiation.…”

Section: Introductionmentioning

confidence: 99%