Investigation of the biotransformation pathway of verapamil using electrochemistry/liquid chromatography/mass spectrometry – A comparative study with liver cell microsomes

Jahn, Sandra; Baumann, A.; Roscher, Jörg; Hense, Katharina; Zazzeroni, Raniero; Kärst, Uwe

doi:10.1016/j.chroma.2011.10.052

Cited by 35 publications

(16 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Obtained results were compared to microsomal incubations and in vivo experiments by analysing urine samples from a patient after tetrazepam delivery. In a verapamil study, a widely prescribed calcium channel blocker, a boron-doped diamond (BDD) electrode was employed due to its extraordinary properties -large potential window, ability to generate hydroxyl radicals and fouling resistance [14]. A similar comparison study for alkaloids galantamine (GAL) and lycorine (LYC) was reported by Jahn et al [15].…”

Section: Drug Metabolismmentioning

confidence: 96%

Hyphenation of Electrochemistry with Mass Spectrometry for Bioanalytical Studies

Cindric

Matysik

2013

Advances in Chemical Bioanalysis

View full text Add to dashboard Cite

Hyphenation of electrochemistry (EC) and mass spectrometry (MS) is a growing research field with particular importance for bioanalytical applications. It opens the way for studying reaction mechanisms and metabolic pathways of biological compounds and drugs. Electrochemical conversion of sample molecules prior to MS analysis gives rise to short-lived intermediates and products naturally occurring in biological systems, which leads to better understanding of physiological processes. Numerous interesting and attractive studies in this field have been published so far demonstrating potential of EC-MS coupling. The combination with separation system such as liquid chromatography or capillary electrophoresis widens the scope of application providing additional information about compounds of interest. The combination of EC with liquid chromatography has been the most frequently used hyphenated system due to the simplicity of coupling to mass spectrometric detection. In terms of bioanalytical applications capillary electrophoresis offers some advantages and is a complementary technique to liquid chromatography. This review summarizes recent developments in this field from both instrumental and application perspectives. A rather new approach of coupling electrochemistry-capillary electrophoresis-mass spectrometry and its potential for bioanalysis is presented.

show abstract

Section: Drug Metabolismmentioning

confidence: 96%

Hyphenation of Electrochemistry with Mass Spectrometry for Bioanalytical Studies

Cindric

Matysik

2013

Advances in Chemical Bioanalysis

View full text Add to dashboard Cite

show abstract

“…In particular, electrodes can be precisely set to impose specific potentials (i.e., voltages) which enable the controlled study of oxidation and reduction reactions: a capability that seems intuitively well matched to the experimental needs in redox biology. For instance, electrochemical oxidation reactions have been used to mimic the cytochrome P450 catalyzed oxidative metabolism of drugs [215][216][217][218], while electrochemical reduction reactions have been used to model the bioreduction of prodrugs [219,220]. Also, electrodes are integral to the shuttling of electrons in bio-based fuel cells which aim to enlist biological mechanisms to convert chemical into electrical energy [221].…”

Section: Redox: Another Global Biological Modality?mentioning

confidence: 99%

Electrochemical reverse engineering: A systems-level tool to probe the redox-based molecular communication of biology

Liu

Kim

et al. 2017

Free Radical Biology and Medicine

View full text Add to dashboard Cite

The intestine is the site of digestion and forms a critical interface between the host and the outside world. This interface is composed of host epithelium and a complex microbiota which is "connected" through an extensive web of chemical and biological interactions that determine the balance between health and disease for the host. This biology and the associated chemical dialogues occur within a context of a steep oxygen gradient that provides the driving force for a variety of reduction and oxidation (redox) reactions. While some redox couples (e.g., catecholics) can spontaneously exchange electrons, many others are kinetically "insulated" (e.g., biothiols) allowing the biology to set and control their redox states far from equilibrium. It is well known that within cells, such non-equilibrated redox couples are poised to transfer electrons to perform reactions essential to immune defense (e.g., transfer from NADH to O for reactive oxygen species, ROS, generation) and protection from such oxidative stresses (e.g., glutathione-based reduction of ROS). More recently, it has been recognized that some of these redox-active species (e.g., HO) cross membranes and diffuse into the extracellular environment including lumen to transmit redox information that is received by atomically-specific receptors (e.g., cysteine-based sulfur switches) that regulate biological functions. Thus, redox has emerged as an important modality in the chemical signaling that occurs in the intestine and there have been emerging efforts to develop the experimental tools needed to probe this modality. We suggest that electrochemistry provides a unique tool to experimentally probe redox interactions at a systems level. Importantly, electrochemistry offers the potential to enlist the extensive theories established in signal processing in an effort to "reverse engineer" the molecular communication occurring in this complex biological system. Here, we review our efforts to develop this electrochemical tool for in vitro redox-probing.

show abstract

“…In the last years, EC has turned out to be a powerful tool especially for the simulation of metabolic reactions. [20][21][22][23][24] By the hyphenation of EC with MS, TPs can directly be detected aer their generation and even shortlived, reactive intermediates can be identied. 25 In conventional in vitro or in vivo assays based on liver cell incubations or the analysis of urine and feces, the detection of reactive species is difficult due to matrix inuence and rapid binding to cell constituents such as proteins or DNA.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the oxidative transformation of roxarsone by electrochemistry coupled to hydrophilic interaction liquid chromatography/mass spectrometry

Frensemeier

Büter

Vogel

et al. 2017

J. Anal. At. Spectrom.

Self Cite

View full text Add to dashboard Cite

show abstract

Investigation of the biotransformation pathway of verapamil using electrochemistry/liquid chromatography/mass spectrometry – A comparative study with liver cell microsomes

Cited by 35 publications

References 49 publications

Hyphenation of Electrochemistry with Mass Spectrometry for Bioanalytical Studies

Hyphenation of Electrochemistry with Mass Spectrometry for Bioanalytical Studies

Electrochemical reverse engineering: A systems-level tool to probe the redox-based molecular communication of biology

Investigation of the oxidative transformation of roxarsone by electrochemistry coupled to hydrophilic interaction liquid chromatography/mass spectrometry

Contact Info

Product

Resources

About