Journal of Chromatography A volume 1218, issue 51, P9210-9220 2011 DOI: 10.1016/j.chroma.2011.10.052 View full text
Sandra Jahn, Anne Baumann, Jörg Roscher, Katharina Hense, Raniero Zazzeroni, Uwe Karst

Abstract: The biotransformation pathway of verapamil, a widely prescribed calcium channel blocker, was investigated by electrochemistry (EC) coupled online to liquid chromatography (LC) and electrospray mass spectrometry (ESI-MS). Mimicry of the oxidative phase I metabolism was achieved in a simple amperometric thin-layer cell equipped with a boron-doped diamond (BDD) working electrode. Structures of the electrochemically generated metabolites were elucidated on the basis of accurate mass data and additional MS/MS exper…

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