Investigation of the association of idiopathic male infertility with polymorphisms in the methionine synthase (MTR) gene

Abstract

ObjectiveSpermatogenesis is a complex process that is regulated by a number of genes, some of which are involved in folate-dependent 1-carbon metabolism. Methionine synthase (encoded by MTR) is a key enzyme participating in this pathway. This study aimed to investigate the relationship of the MTR 2756A > G polymorphism with idiopathic male fertility in the Iranian population.MethodsThe participants of this study included 100 men with idiopathic infertility and 100 healthy men as the control group. Genotyping of MTR 2756A > G was performed using the polymerase chain reaction and restriction fragment length polymorphism technique. The obtained data were analyzed using SPSS ver. 20.0 with a level of confidence of p< 0.05.ResultsThe frequencies of the A and G alleles at this locus were 77% and 23% in infertile patients and 84% and 16% in the control group, respectively. The frequencies of the GG, GA, and AA genotypes were 5%, 36%, and 59% in the infertile patients versus 3%, 27%, and 70% in the control group, respectively. No significant difference was observed in any genetic models.ConclusionIn general, the findings of this study suggest that the MTR 2756A > G single-nucleotide polymorphism is not a predisposing factor for idiopathic infertility in men.

The Association Study between Tumor Necrosis Factor- Receptor 1 36 A/G (TNFR1 36 A/G) Gene Polymorphism and Azoospermic Infertile Men in Erbil City

“…The genetic factors and environmental have a role in infertility, and the genetic factors are confirmed in men with idiopathic infertility (Tanoomand et al, 2019). Table 1, showed that the odds ratio for AG groups was more than one and the p-value was 0.014; it means that this group has increased risk to azoospermia.…”

Methionine synthase A2756G polymorphism associated with an increased risk of head and neck cancer

“…32 MTR gene which encodes methionine synthase enzyme, has significant role in folic acid metabolism. 33 Methionine synthase, an enzyme responsible for catalysing the methylation of homocysteine to methionine, a significantly important reaction that regulates normal homeostasis of methionine and intracellular homocysteine concentrations. 34,16 As far as we know, the functional activity of methionine synthase enzyme in the presence of the MTR-A2756G polymorphism has not been assessed in vitro.…”

Association of MTR A2756G and MTRR A66G Polymorphisms With Male Infertility: An Updated Meta-Analysis

“…Some mutations result in defective enzymes in the folate metabolism pathway, which are linked to male infertility (K. Liu et al, 2015). Single nucleotide polymorphisms (SNPs) in MTRR A66G (rs1801394) and MTR A2756G (rs1805087) have been reported (Karimian & Colagar, 2016;Kurzawski et al, 2015;Lee et al, 2006;Tanoomand et al, 2019) which can alter the amount, stability, and activity of enzymes involved in folate metabolism, which could have an impact on DNA synthesis and folate metabolism (Z. J. Ren et al, 2017).…”

“…The World Health Organization (WHO) defines infertility as a couple’s inability to conceive or carry a pregnancy following a year of routine, unprotected sexual activity (Balkan et al, 2013; Tanoomand et al, 2019; Tüttelmann et al, 2007). The prevalence of male fertility issues is between 40% and 50% (Balkan et al, 2013).…”

“…Several genes regulate the intricate process of spermatogenesis, and some of these genes are implicated in the route of folate metabolism (Tanoomand et al, 2019). Folates are interconvertible co-enzymes that help in preserving the stability and integrity of the genome by helping in DNA synthesis, repair, and methylation.…”