Investigation of the antioxidant and aldose reductase inhibitory activities of extracts from Peruvian tea plant infusions

Wang, Zhiqiang; Hwang, Seung Hwan; Quispe, Yanymee N. Guillen; Gonzáles, Paúl; Lim, Soon Sung

doi:10.1016/j.foodchem.2017.03.107

Cited by 45 publications

(40 citation statements)

References 30 publications

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“…An ultrafiltration-HPLC assay was performed in this study to identify the potential α-glucosidase inhibitors in HL. The ultrafiltration-HPLC assay is an affinity-based screening method for bioactive compounds in a complex matrix whereby the ligands bind to the enzyme and are separated from unbound small molecules by the ultrafiltration membrane [ 34 ]. Thus, examination of the HPLC pattern of the decreased peaks indicates which ligands would be suitable as inhibitors.…”

Section: Resultsmentioning

confidence: 99%

“…The disappearance of some peaks after incubation was attributed to the low concentration of some components. However, in the case of quercetin, this was attributed to its solubility, binding affinities, and binding sites [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

“…The DPPH free radical scavenging activity was determined as previously described with slight modifications [ 34 ]. Briefly, 180 µL of DPPH solution (0.32 mM in methanol) was mixed with 30 µL of each sample at different concentrations (1.0–5.0 mg/mL in methanol).…”

Section: Methodsmentioning

confidence: 99%

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Screening In Vitro Targets Related to Diabetes in Herbal Extracts from Peru: Identification of Active Compounds in Hypericum laricifolium Juss. by Offline High-Performance Liquid Chromatography

Quispe

Hwang

Wang

et al. 2017

IJMS

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This study investigates in vitro targets related to diabetes in 30 herbal extracts from Peru, for the first time, using α-glucosidase, aldose reductase (AR) inhibitory assays and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging assays. Among the 30 herbal extracts, Hypericum laricifolium Juss. (HL) was the herb which showed more than 50% inhibition in all assays, presenting 97.2 ± 2.0%, 56.9 ± 5.6%, 81.9 ± 2.5%, and 58.8 ± 4.6% inhibition for the α-glucosidase, AR, DPPH, and ABTS assays, respectively. Finally, six bioactive compounds, namely, protocatechuic acid, chlorogenic acid, caffeic acid, kaempferol 3-O-glucuronide, quercetin, and kaempferol were identified in HL by offline high-performance liquid chromatography (HPLC). Quercetin exhibited the strongest inhibition in all enzyme assays and the strongest antioxidant activity. The results suggest that HL shows great potential for the complementary treatment of diabetes and its complications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Screening In Vitro Targets Related to Diabetes in Herbal Extracts from Peru: Identification of Active Compounds in Hypericum laricifolium Juss. by Offline High-Performance Liquid Chromatography

Quispe

Hwang

Wang

et al. 2017

IJMS

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“…Plant-based sources such as fruits and vegetables have increasingly gained the interest of scientists in search of naturally-existing antioxidants (Wang et al, 2017;Nile et al, 2018). These sources exhibit strong biological activity and are easily available.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Anti-inflammatory Properties of Raw and Processed Fruits and Vegetables

Lee

Saba

Kim

et al. 2018

BSL

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Reactive oxygen species (ROS) generated from metabolic reactions cause oxidative DNA damage, which results in oxidative tissue injury. Therefore, there is an increasing demand in the intake of high antioxidant sources in order to maintain a healthy environment in cells. In this study, we investigated the antioxidant and anti-inflammatory activities of Malus domestica (apple), Pyrus communis L. (pear), Daucus carota L. (carrot), Brassica oleracea var. (broccoli), Brassica oleracea var. capitata (cabbage), and Raphanus sativus L. (radish) obtained from the local market. Since these are common fruits and vegetables that are widely consumed, we aimed to investigate their beneficial properties, placing particular emphasis on their antioxidant and anti-inflammatory properties. The samples were processed via an indirect heating method and their properties were compared to their raw forms. Based on DPPH and ABTS assays, processed samples showed better antioxidant activities when compared to raw samples and processed pear samples exhibited the best antioxidant activity. The anti-inflammatory activities of the samples were also investigated in LPS-treated RAW 264.7 cells. mRNA expression of pro-inflammatory mediators and cytokines (iNOS, COX-2, TNF-α, IL-1β, and IL-6) was assessed using RT-PCR. As expected, processed samples exhibited better iNOS inhibition when compared to their raw forms and processed broccoli and cabbage samples exhibited outstanding anti-inflammatory effects. The samples, up to 1 mg/mL concentration, did not exhibit cytotoxicity against RAW 264.7 cells as demonstrated by cell viability assays. Altogether, processed broccoli and cabbage samples exhibited the strongest anti-inflammatory properties.

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“…Igualmente se ha identificado derivados de flavonas como naringenina, derivados de ácido hidroxicinamico como el ácido cumarico y ácido clorogénico, demostrándose que las hojas de L.meyeni tienen una elevada actividad antioxidante (5,6) .…”

Section: Mensajes Clave Introducciónunclassified

Efecto antiiflamatorio de la fracción flavonoide de Lepechinia meyenii (Walp.) Epling (Salvia) sobre leucocitos de pacientes con artritis reumatoide

Arenas-Chávez

Wiche-Salinas

Valencia-Mercado

et al. 2018

Rev Peru Med Exp Salud Publica

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Investigation of the antioxidant and aldose reductase inhibitory activities of extracts from Peruvian tea plant infusions

Cited by 45 publications

References 30 publications

Screening In Vitro Targets Related to Diabetes in Herbal Extracts from Peru: Identification of Active Compounds in Hypericum laricifolium Juss. by Offline High-Performance Liquid Chromatography

Screening In Vitro Targets Related to Diabetes in Herbal Extracts from Peru: Identification of Active Compounds in Hypericum laricifolium Juss. by Offline High-Performance Liquid Chromatography

Antioxidant and Anti-inflammatory Properties of Raw and Processed Fruits and Vegetables

Efecto antiiflamatorio de la fracción flavonoide de Lepechinia meyenii (Walp.) Epling (Salvia) sobre leucocitos de pacientes con artritis reumatoide

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