Investigation of the Antimicrobial Activity of Bacillus licheniformis Strains Isolated from Retail Powdered Infant Milk Formulae

Álvarez‐Ordóñez, Avelino; Begley, Máire; Clifford, Tanya; Deasy, Thérèse; Considine, Kiera; O’Connor, Paula M.; Ross, R. Paul; Hill, Colin

doi:10.1007/s12602-013-9151-1

Cited by 15 publications

(12 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, B. licheniformis is used for several biotechnological applications for its capacity to produce degrading enzymes such as proteases, lipases, pectate lyases, and polysaccharides [ 59 ]. B. licheniformis has further been shown to exhibit potent antimicrobial activity against indicator strains ( Lactococcus lactis , Lactobacillus bulgaricus and Listeria innocua ) and is clinically relevant ( Listeria monocytogenes , Staphylococcus aureus , Streptococcus agalactiae , Salmonella Typhimurium and Escherichia coli ) bacteria [ 60 ]. B. subtilis is a recognized producer of natural biocontrol agents, having high antifungal activity against Alternaria solani , Botrytis cinerea , Monilia linhartiana 869, Phytophthora cryptogea 759/1 and Rhizoctonia sp., and antibacterial activity against Pseudomonas syringae pv.…”

Section: Resultsmentioning

confidence: 99%

Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential

et al. 2016

View full text Add to dashboard Cite

Microorganisms that inhabit unchartered unique soil such as in the highly saline and hot Red Sea lagoons on the Saudi Arabian coastline, represent untapped sources of potentially new bioactive compounds. In this study, a culture-dependent approach was applied to three types of sediments: mangrove mud (MN), microbial mat (MM), and barren soil (BS), collected from Rabigh harbor lagoon (RHL) and Al-Kharrar lagoon (AKL). The isolated bacteria were evaluated for their potential to produce bioactive compounds. The phylogenetic characterization of 251 bacterial isolates based on the 16S rRNA gene sequencing, supported their assignment to five different phyla: Proteobacteria, Firmicutes, Actinobacteria, Bacteroidetes, and Planctomycetes. Fifteen putative novel species were identified based on a 16S rRNA gene sequence similarity to other strain sequences in the NCBI database, being ≤98%. We demonstrate that 49 of the 251 isolates exhibit the potential to produce antimicrobial compounds. Additionally, at least one type of biosynthetic gene sequence, responsible for the synthesis of secondary metabolites, was recovered from 25 of the 49 isolates. Moreover, 10 of the isolates had a growth inhibition effect towards Staphylococcus aureus, Salmonella typhimurium and Pseudomonas syringae. We report the previously unknown antimicrobial activity of B. borstelensis, P. dendritiformis and M. salipaludis against all three indicator pathogens. Our study demonstrates the evidence of diverse cultured microbes associated with the Red Sea harbor/lagoon environments and their potential to produce antimicrobial compounds.

show abstract

Section: Resultsmentioning

confidence: 99%

Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential

et al. 2016

View full text Add to dashboard Cite

show abstract

“…We identified these isolates by 16S rDNA sequencing and revealed that B. licheniformis were more prevalent than other Bacillus species, and were found in raw rice, raw milk, infant fecal samples and infant milk formula. B. licheniformis have been recovered from raw and infant milk powder, and implicated in food poisoning cases [31,32] . B. licheniformis have shown to exhibit pathogenic properties and they may pose a health hazard [33,34] .…”

Section: Discussionmentioning

confidence: 99%

Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces

Organji

Abulreesh

Elbanna

et al. 2015

Asian Pacific Journal of Tropical Biomedicine

View full text Add to dashboard Cite

Objective: To investigate the true incidence of Bacillus cereus (B. cereus) in food and children diarrhea cases. Methods: A total of 110 samples of various dairy products such as raw milk, long life pasteurized milk, yoghurt and infant powdered milk formulas, raw rice, and feces were examined for the presence of B. cereus by selective plating on mannitol-egg-yolkpolymyxin agar. Confirmation of B. cereus was carried out by biochemical tests and PCR. Identification of non-B. cereus isolates was carried out by 16S rDNA sequencing. Antimicrobial susceptibility was done by disk diffusion method. Results: Overall 35 samples (31.8%, n = 110) yielded Bacillus-like growth. Of which 19 samples (54.28%) were positive for B. cereus. All isolates were positive for enterotoxin production. No psychrotolerant B. cereus strains were detected in all samples. All B. cereus isolates were resistant to penicillin G, but susceptible to vancomycin, erythromycin and clindamycin. Conclusions: The results of this study confirm the importance of including B. cereus in disease control and prevention programs, as well as in routine clinical and food quality control laboratories in both Saudi Arabia and Egypt.

show abstract

“…Lichenicidin is a lantibiotic that has broad antibacterial activity against Gram-positive organisms (Dischinger et al , 2009). A recent study of isolates of B. licheniformis from powdered infant formula found that the group could be partitioned into lichenicidin producers and non-producers that had greater antibacterial activity (Alvarez-Ordóñez et al , 2014). Group 2 strains contained some low-similarity orthologues to some of the lichenicidin genes found in group 1, specifically LanT, LanM and a LanA-like protein with protein sequence similarities of 48, 33 and 43 %, respectively.…”

mentioning

confidence: 99%

Bacillus paralicheniformis sp. nov., isolated from fermented soybean paste

Dunlap

Kwon

Rooney

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

An isolate of a Gram-stain-positive, facultatively anaerobic, motile, rod-shaped, endosporeforming bacterium was recovered from soybean-based fermented paste. Phylogenetic analysis of the 16S rRNA gene indicated that the strain was most closely related to Bacillus sonorensis KCTC-13918 T (99.5 % similarity) and Bacillus licheniformis DSM 13 T (99.4 %). In phenotypic characterization, the novel strain was found to grow at 15-60 8C and to tolerate up to 10 % (w/v) NaCl. Furthermore, the strain grew in media with pH 6-11 (optimal growth at pH 7.0-8.0). The predominant cellular fatty acids were anteiso-C 15 : 0 (37.7 %) and iso-C 15 : 0 (31.5 %). The predominant isoprenoid quinone was menaquinone 7 (MK-7). The cell-wall peptidoglycan contained meso-diaminopimelic acid. A draft genome sequence of the strain was completed and used for phylogenetic analysis. Phylogenomic analysis of all published genomes of species in the B. licheniformis group revealed that strains belonging to B. licheniformis clustered into two distinct groups, with group 1 consisting of B. licheniformis DSM 13 T and 11 other strains and group 2 consisting of KJ-16 T and four other strains. The DNA G+C content of strain KJ-16 T was 45.9 % (determined from the genome sequence). Strain KJ-16 T and another strain from group 2 were subsequently characterized using a polyphasic taxonomic approach and compared with strains from group 1 and another closely related species of the genus Bacillus. Based upon the consensus of phylogenetic and phenotypic analyses, we conclude that this strain represents a novel species within the genus Bacillus, for which the name Bacillus paralicheniformis sp. nov. is proposed, with type strain KJ-16 T (5KACC T 5NRRL B-65293 T ).Cheonggukjang is a fermented soybean product in traditional Korean cuisine. It is produced by boiling soybeans and allowing them to ferment for 2-3 days in the presence of a 'natural' environmental inoculum present in the air or sometimes added through the addition of rice straw (Cho et al., 2014). The bacterial diversity of the cheonggukjang inoculum is known to be dominated by members of the genus Bacillus (Nam et al., 2012), some of which have been identified previously (e.g. Bacillus subtilis), whereas others have yet to be formally described. The purpose of this study was to describe one such species, for which we propose the name Bacillus paralicheniformis sp. nov.Strain KJ-16 T was isolated from a 3-day-old cheonggukjang fermentation on R2A agar at 28 8C. Growth was tested at 4, 10, 15, 28, 37, 45, 55 and 60 8C. The pH range for growth was determined from pH 3.0 to 12.0 in steps of 1.0 pH unit in R2A broth buffered with 0.1 M citrate/phosphate buffer (pH 3-7), Tris (pH 8, 9), NaCO 3 (pH 10, 11) or phosphate (pH 12) and adjusted with HCl or NaOH as needed (Breznak & Costilow, 1994). NaCl tolerance was investigated by using R2A broth supplemented with 0, 1, 2, 3, 4, 5, 7 and 10 % (w/v) NaCl. Growth under anaerobic conditions was determined on anaerobic agar (Difco) at 30 8C using a GasPak jar (,...

show abstract

Investigation of the Antimicrobial Activity of Bacillus licheniformis Strains Isolated from Retail Powdered Infant Milk Formulae

Cited by 15 publications

References 24 publications

Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential

Bioprospecting Red Sea Coastal Ecosystems for Culturable Microorganisms and Their Antimicrobial Potential

Occurrence and characterization of toxigenic Bacillus cereus in food and infant feces

Bacillus paralicheniformis sp. nov., isolated from fermented soybean paste

Contact Info

Product

Resources

About