Investigation of the anti‐inflammatory and analgesic effects from an extract of Aplysina caissara, a marine sponge

Abstract

A variety of biologically active compounds with pharmacological applications has been reported to occur in marine sponges. The present study was undertaken to provide a set of data about an extract from Aplysina caissara, a Brazilian marine sponge. The antinociceptive and anti-inflammatory effects were investigated against different experimental models in mice. When evaluated against writhing test intraperitoneally (60 and 90 mg/kg), the extract significantly inhibited abdominal constriction by 33.7% and 41.4% respectively. In the formalin test (60 and 90 mg/kg), the extract of sponge inhibited 43.6% and 51.6% in the first phase and 98.2% and 97.2% in the second phase respectively. When evaluated against the hot plate test, both doses demonstrated activity. An increase in the hot plate latency was observed after 60 min. The anti-inflammatory effect was evaluated by formalin-induced mice paw edema. Extract from A. caissara (60 and 90 mg/kg) significantly reduced hind paw swelling. Mortality increased with increasing doses, with LD(50) of 212.2 mg/kg for intraperitoneal administration. These results demonstrated that the extract of the marine sponge A. caissara possesses antinociceptive and anti-edematogenic effects.

Antinociceptive and Anti-inflammatory Activities of Marine Sponges Aplysina Caissara, Haliclona sp. and Dragmacidon Reticulatum

“…After filtration, the extracted material was concentrated in a rotary evaporator (Fisaton, Brazil) and the final extract was partitioned against hexane (1 : 1 v/v). The final extract was dried in SpeedVac (SPD1010; ThermoSavant, NY, USA) 13 .…”

“…The specie A. caissara, an endemic Brazilian specie of marine sponges, is rich in active peptides and alkaloids 21,22 . Our laboratory studies have indicated that aqueous extracts of A. caissara, administered intraperitoneally show antinociceptive and anti-inflammatory profiles 13 . Besides, the anti-tuberculosis and cytotoxic activity was also shown in vitro 22 .…”

“…The analgesic potential of sponges is less explored and was already evidenced for Aplysina caissara. 13 Our study was conducted with three species of marine sponges, Haliclona sp., Dragmacidon reticulatum and Aplysina caissara. Sponges of the genus Haliclona are well-known for producing a variety of secondary metabolites, many of them are cytotoxic 14,15 , antifungal 16 , antimicrobial 17 and anti-inflammatory 18,19 .…”

Bioprospecting of Marine Sponge (Callyspongia Diffusa) for Antibacterial Compound.

“…Marine sponges are rich sources of pharmacological compounds [4]. Studies show that secondary metabolites of sponges play an important role in their survival in the marine water [5,6].…”

Suppression of cyclooxygenase-2 and inducible nitric oxide synthase expression by epimuqubilin A via IKK/IκB/NF-κB pathways in lipopolysaccharide-stimulated RAW 264.7 cells

“…More recently, some compounds have been entered into clinical trials as anticancer drug candidates, including eribulin mesylate (E7389) in a phase III trial, and E7974 (hemiasterlin) in phase I trial (Mayer et al, 2010). Some extracts or compounds from marine sponges have been reported to inhibit the inflammation processes, including an ethyl acetate fraction from Cliona celata (Yang et al, 2010), the extract from Aplysina caissara (Azevedo et al, 2008), halichlorine (Tsubosaka et al, 2010), and heteronemin (Schumacher et al, 2010). We have demonstrated the anti-inflammatory potential of epimuqubilin A, a norsesterterpene peroxide isolated from marine sponge Latrunculia sp.…”