Investigation of stress tolerance of Pichia kudriavzevii for high gravity bioethanol production from steam–exploded wheat straw hydrolysate

Hoppert, Luis; Kölling, Ralf; Einfalt, Daniel

doi:10.1016/j.biortech.2022.128079

Cited by 8 publications

(4 citation statements)

References 42 publications

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“…Although several lignocellulosic pretreatment processes have been reported, the chemical process using dilute acid is typically used since it has high efficiency in separating cell wall components, yielding high concentrations of fermentable sugars. It is also a simple process with a low-cost operation that can be adopted for wide-scale applications compared to physicochemical and biological processes 78 – 80 . In this study, dilute acid pretreatment of sugarcane bagasse using 3% (v/v) sulfuric acid at 121 °C and 15 psi for 60 min was performed, yielding a liquid fraction called acid hydrolysate, as mentioned in the Materials and Methods section.…”

Section: Resultsmentioning

confidence: 99%

“…Phong et al 39 reported a maximum ethanol concentration of 36.91 g/L from dilute acid hydrolysate of pineapple waste containing 103 g/L total sugar at 45 °C. Recently, Hoppert et al 80 demonstrated that P. kudriavzevii HYPK213_ELA could produce a maximum ethanol concentration of 56.8 g/L from wheat straw hydrolysate at 37% w/w solid loading at 40 °C. Regarding the ethanol production in the current study, a low ethanol concentration obtained from a mixture of acid and enzymatic hydrolysate of sugarcane bagasse by P. kudriavzevii evolved strains may be due to a low sugar concentration in the fermentation medium.…”

Section: Resultsmentioning

confidence: 99%

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Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass

Dolpatcha,

Phong,

Thanonkeo

et al. 2023

Sci Rep

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Second-generation bioethanol production using lignocellulosic biomass as feedstock requires a highly efficient multistress-tolerant yeast. This study aimed to develop a robust yeast strain of P. kudriavzevii via the adaptive laboratory evolution (ALE) technique. The parental strain of P. kudriavzevii was subjected to repetitive long-term cultivation in medium supplemented with a gradually increasing concentration of acetic acid, the major weak acid liberated during the lignocellulosic pretreatment process. Three evolved P. kudriavzevii strains, namely, PkAC-7, PkAC-8, and PkAC-9, obtained in this study exhibited significantly higher resistance toward multiple stressors, including heat, ethanol, osmotic stress, acetic acid, formic acid, furfural, 5-(hydroxymethyl) furfural (5-HMF), and vanillin. The fermentation efficiency of the evolved strains was also improved, yielding a higher ethanol concentration, productivity, and yield than the parental strain, using undetoxified sugarcane bagasse hydrolysate as feedstock. These findings provide evidence that ALE is a practical approach for increasing the multistress tolerance of P. kudriavzevii for stable and efficient second-generation bioethanol production from lignocellulosic biomass.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass

Dolpatcha,

Phong,

Thanonkeo

et al. 2023

Sci Rep

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“…Every coin has two sides. For instance, although Pichia kudriavzeveii is a globally distributed opportunistic pathogenic yeast (Pfaller et al, 2008), whose infections frequently acquired from the environment was verified by Douglass et al (2018), it plays a significant role in bioethanol production (Hoppert et al, 2022), cocoa fermentation (Pereira et al, 2017), single−cell protein production (Hashem et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Diversity and distribution of yeasts in intertidal zones of China

Zhu,

Han,

Guo

et al. 2023

Front. Mar. Sci.

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China has the second greatest extent of intertidal zones in the world. The intertidal zone is the most dynamic environments in the biosphere and potentially supports high biodiversity. Marine yeasts show excellent performance in various industrial, environmental and medical applications, however, the marine yeast diversity has rarely been studied in China. In this study, we collected 1241 samples including marine sediments, marine water, plants, and benthos at 161 GPS sites in different types of intertidal zones along the Chinese coastline from north to south. A total of 4436 strains were isolated from these samples using different methods and 286 species including 39 potential novel species were identified from these strains based on the internal transcribed spacer (ITS) region or the D1/D2 domain of the large subunit rRNA gene sequence analysis. The majority of the yeast species in different geographical locations belong to the five orders Serinales, Saccharomycetales, Tremellales, Sporidiobolales, and Pichiales. The yeast species diversity varied depending on sample types, depth of marine sediments, intertidal zone types and geographical locations. Mean annual temperature (MAT), salinity and pH had the greatest effect on the community structures of the yeasts isolated from the intertidal zones. This study represents one of the most comprehensive surveys of marine yeasts in China to date and provides a better understanding of marine yeast diversity and distribution.

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“…Bioethanol production has been proposed and developed using various agricultural biomasses, including corn [ 3 ], sugarcane [ 4 ], sugar beet [ 5 ], potato [ 6 ], and wheat [ 7 ]. Compared with fossil fuels, bioethanol produces fewer toxic substances and causes fewer harmful environmental issues [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Utilization of Macroalgae for the Production of Bioactive Compounds and Bioprocesses Using Microbial Biotechnology

Shibasaki

Ueda

2023

Microorganisms

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To achieve sustainable development, alternative resources should replace conventional resources such as fossil fuels. In marine ecosystems, many macroalgae grow faster than terrestrial plants. Macroalgae are roughly classified as green, red, or brown algae based on their photosynthetic pigments. Brown algae are considered to be a source of physiologically active substances such as polyphenols. Furthermore, some macroalgae can capture approximately 10 times more carbon dioxide from the atmosphere than terrestrial plants. Therefore, they have immense potential for use in the environment. Recently, macroalgae have emerged as a biomass feedstock for bioethanol production owing to their low lignin content and applicability to biorefinery processes. Herein, we provided an overview of the bioconversion of macroalgae into bioactive substances and biofuels using microbial biotechnology, including engineered yeast designed using molecular display technology.

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Investigation of stress tolerance of Pichia kudriavzevii for high gravity bioethanol production from steam–exploded wheat straw hydrolysate

Cited by 8 publications

References 42 publications

Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass

Adaptive laboratory evolution under acetic acid stress enhances the multistress tolerance and ethanol production efficiency of Pichia kudriavzevii from lignocellulosic biomass

Diversity and distribution of yeasts in intertidal zones of China

Utilization of Macroalgae for the Production of Bioactive Compounds and Bioprocesses Using Microbial Biotechnology

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