background: Dentists are prone to professional burnout due to the nature of their work but this phenomenon may be prevented. Professional burnout has a great impact on different aspects of human life. On the other hand, temperament could have a vital role in behaviors, interests, and lifestyle. The present study aimed to determine the professional burnout level among Iranian dentists and its association with temperament in view of traditional Persian medicine and work-related variables. Methods: This observational study with a cross-sectional design was conducted among an Iranian dentist sample in Yazd between February 2020 and August 2020. 145 dentists were enrolled in the study. Human temperament and burnout levels were assessed by means of the Salmannejad temperament and Maslach Burnout Inventory questionnaires (MBI), respectively. In total, 120 valid questionnaires were collected, with a response rate of 82.76%. Data analysis was performed using descriptive statistics mean and standard deviation, Analytical tests (including chi-square test, multivariate analysis of variance) using Spss17 (Chicago, USA) software was done.Results: Among participants, 8.3% had high emotional exhaustion, 7.5% had depersonalization and 2.5% had reduced personal accomplishment. According to the results, 28.3% of dentists had warm-wet, 13.3% had warm-dry, 7.5% had cold-dry and 13.3% had cold-wet mizaj. The score of professional burnout in the area of emotional exhaustion had a positive correlation with job satisfaction. (P-value<0.05)Conclusion: Based on the findings, it is concluded, it may conclude professional burnout and job satisfaction had a negative significant correlation. No statistically significant relationship was seen between different mizaj and professional burnout among dentists.