Investigation of stimulated growth effect using pulsed cold atmospheric plasma treatment on Ganoderma lucidum

Abstract

Cold atmospheric plasma (CAP) driven by pulsed high-voltage power has specific advantages in various fields, such as the growth promotion of edible fungi in plasma agriculture. Compared with other strains, Ganoderma lucidum has the significant advantage of high medicinal value, but the shortcomings of low yield, long growth cycle, and an uneven market quality. In this study, Ganoderma lucidum was treated with a CAP powered by a homemade pulsed high-voltage power supply. Three groups of Ganoderma lucidum were treated under different conditions. The stalk lengths and pileus areas of Ganoderma lucidum were recorded to evaluate growth status. Results showed that treatment frequency affected growth status considerably. Ganoderma lucidum was treated with 40 s plasma once a week and showed improved growth status with a 33.63% increase in average stalk length and a 28% increase in the number of individuals whose pileus areas was greater than the average. Meanwhile, the growth speed was accelerated. However, treatment at an excessive frequency would damage and slow down the growth of Ganoderma lucidum.

Recent progress of atmospheric and room‐temperature plasma as a new and promising mutagenesis technology

“…The expansion of ARTP mutagenesis application in obtaining polysaccharideenhanced Ganoderma mutants was also demonstrated. 78 Zhang's group, 149 significantly increased the intracellular polysaccharide production and flavonoid content of Morus alba strains by ARTP mutagenesis. It was also noted that ARTP was used to increase polysaccharide production by increasing the molecular weight and in vitro macrophage activation activity in the Morus alba strain.…”

“…[74][75][76] In recent years, an increasing number of researchers have found that sublethal effects on cells can be achieved by controlling the power and treatment time of ARTP, 11 and beyond a certain range, lethal effects can occur, thus extending the application of biological mutagenesis. 77,78 The different biological effects resulting from the interaction between ARTP and organisms are achieved through a series of complex reactive processes. Li et al, 25 introduced the genetic effect of atmospheric pressure helium RF plasma discharge in detail, and found that it was mainly some highly chemically reactive chemical species, such as hydroxyl radical (•OH), oxygen radical (•O), singlet oxygen ( 1 O 2 ), and nitric oxide (NO), which provided the feasibility for using ARTP to mutate organisms.…”

“…Mutagenesis of production strains through ARTP technology has often been widely studied for its ability to significantly increase strain growth rates, target product yields, and economic benefits. 17,78,[149][150][151] Polysaccharide is an active substance contained in the monkey head mushroom that is commonly used in the treatment of diseases such as neurological disorders and tumors.…”

“…In previous studies, the application of ARTP to organisms has been more about lethal damage and the inactivation of organisms 74–76 . In recent years, an increasing number of researchers have found that sublethal effects on cells can be achieved by controlling the power and treatment time of ARTP, 11 and beyond a certain range, lethal effects can occur, thus extending the application of biological mutagenesis 77,78 . The different biological effects resulting from the interaction between ARTP and organisms are achieved through a series of complex reactive processes.…”

“…As human living standards continue to improve, the number of functional foods and pharmaceuticals are of increasing interest, particularly through the production of natural strains of mushrooms, including Monkey Head Mushroom , Ganoderma Lucidum , and Moringa . Mutagenesis of production strains through ARTP technology has often been widely studied for its ability to significantly increase strain growth rates, target product yields, and economic benefits 17,78,149–151 . Polysaccharide is an active substance contained in the monkey head mushroom that is commonly used in the treatment of diseases such as neurological disorders and tumors.…”