Investigation of SPR and electrochemical detection of antigen with polypyrrole functionalized by biotinylated single-chain antibody: A review

Lê, H.Q.A.; Sauriat-Dorizon, Hélène; Korri-Youssoufi, Hafsa

doi:10.1016/j.aca.2010.06.008

Cited by 47 publications

(13 citation statements)

References 40 publications

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“…To date, many antibody-based analytical approaches have been developed, which include ELISA, SPR, flow cytometry, and hand-held immunochromatographic assay [1][2][3][4][5][6][7][8][9][10][11]. We have mainly focused on the preparation of monoclonal antibodies against natural products and the application in ELISA for the serial determination of bioactive compounds in medicinal plants in order to select high quality strains after breeding [12].…”

Section: Discussionmentioning

confidence: 99%

“…To date, the antibody-based analytical method has been expanded from enzyme-linked immunosorbent assay (ELISA) [1], surface plasmon resonance (SPR) [2,3], flow cytometry [4,5], hand-held immunochromatographic assay [6][7][8] to novel eastern blotting techniques [9][10][11]. Among them, ELISA has a great advantage in quantitative and qualitative analysis of bioactive natural products since multi-sample can be continuously determined /detected in the one plate.…”

Section: Introductionmentioning

confidence: 99%

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Fluobodies against Bioactive Natural Products and their Application in Fluorescence-Linked Immunosorbent Assay

et al. 2012

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An enzyme-linked immunosorbent assay (ELISA) using monoclonal antibody (MAb), Fab antibody, and single-chain variable fragment (scFv) antibody has become one of the most promising analytical methods owing to its rapidity, sensitivity, and reliability. Recently, a chimera of green fluorescent protein (GFP) with a scFv antibody, named fluobody, was proposed as a probe for an alternative immunosorbent assay; i.e., fluorescence-linked immunosorbent assay (FLISA). In this FLISA, an even more sensitive, simple, and rapid immunoassay can be performed by detecting the highly sensitive fluorophore of GFP that is genetically and directly fused to the scFv antibody. In addition, the time- and cost-consuming secondary antibody reaction and the following enzyme-substrate reaction, necessary for conventional ELISA, can be avoided, making it possible to complete the assay more rapidly. Focusing on naturally occurring bioactive products, fluobody recognizing 1,4-naphthoquinone, plumbagin and triterpenoid saponin, ginsenosides were successfully expressed in Escherichia coli (E. coli) and applied to FLISA. The construction, the expression, and the potential use of fluobody in quantitative/qualitative analysis of bioactive natural products are reviewed in this article

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fluobodies against Bioactive Natural Products and their Application in Fluorescence-Linked Immunosorbent Assay

et al. 2012

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“…Examples include the immobilization of enzymes [12][13][14], DNA [15], and antibodies [16] onto polypyrrole to perform selective detection of chosen analytes. Another interesting feature of polypyrrole and its derivatives is their improved biocompatibility in comparison with bare metallic medical devices [17], turning these materials into excellent choices for a wide range of bioapplications.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the performance of polypyrrole nanowires on the electrochemical sensing of ammonia in solution

Massafera

Torresi

2012

Journal of Electroanalytical Chemistry

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a b s t r a c tThis work encompasses the direct electrodeposition of polypyrrole nanowires onto Au substrates using different electrochemical techniques: normal pulse voltammetry (NPV) and constant potential method with the aim in applying these films for the first time in ammonia sensing in solution. The performance of these nanowire-based sensors are compared and evaluated in terms of: film morphology (analyzed with scanning electron microscopy); their sensitivity towards ammonia; electrochemical and contact angle measurements. For nanowires prepared by NPV, the sensitivity towards ammonia increases with increasing amount of electrodeposited polypyrrole, as expected due to the role of polypyrrole as electrochemical transducer for ammonia oxidation. On the other hand, nanowires prepared potentiostatically displayed an unexpected opposite behavior, attributed to the lower conductivity of longer polypyrrole nanowires obtained through this technique. These results evidenced that the analytical and physico-chemical features of nanostructured sensors can differ greatly from those of their conventional bulky analogous.

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“…This can be enhanced by tuning the bio/chemical properties of the sensing interface via the addition of, for example, biomolecules (e.g. antibodies)[1, 2] or chemical ligands. [3, 4] Although this strategy has been widely explored, the methods used to modify the surface are often complex and time consuming requiring, for example, synthesis of novel functional monomers and/or a sequence of activation/conjugation steps.…”

Section: Introductionmentioning

confidence: 99%

Doping Polypyrrole Films with 4-N-Pentylphenylboronic Acid to Enhance Affinity towards Bacteria and Dopamine

et al. 2016

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Here we demonstrate the use of a functional dopant as a fast and simple way to tune the chemical affinity and selectivity of polypyrrole films. More specifically, a boronic-functionalised dopant, 4-N-Pentylphenylboronic Acid (PBA), was used to provide to polypyrrole films with enhanced affinity towards diols. In order to prove the proposed concept, two model systems were explored: (i) the capture and the electrochemical detection of dopamine and (ii) the adhesion of bacteria onto surfaces. The chemisensor, based on overoxidised polypyrrole boronic doped film, was shown to have the ability to capture and retain dopamine, thus improving its detection; furthermore the chemisensor showed better sensitivity in comparison with overoxidised perchlorate doped films. The adhesion of bacteria, Deinococcus proteolyticus, Escherichia coli, Streptococcus pneumoniae and Klebsiella pneumoniae, onto the boric doped polypyrrole film was also tested. The presence of the boronic group in the polypyrrole film was shown to favour the adhesion of sugar-rich bacterial cells when compared with a control film (Dodecyl benzenesulfonate (DBS) doped film) with similar morphological and physical properties. The presented single step synthesis approach is simple and fast, does not require the development and synthesis of functional monomers, and can be easily expanded to the electrochemical, and possibly chemical, fabrication of novel functional surfaces and interfaces with inherent pre-defined sensing and chemical properties.

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Investigation of SPR and electrochemical detection of antigen with polypyrrole functionalized by biotinylated single-chain antibody: A review

Cited by 47 publications

References 40 publications

Fluobodies against Bioactive Natural Products and their Application in Fluorescence-Linked Immunosorbent Assay

Fluobodies against Bioactive Natural Products and their Application in Fluorescence-Linked Immunosorbent Assay

Evaluating the performance of polypyrrole nanowires on the electrochemical sensing of ammonia in solution

Doping Polypyrrole Films with 4-N-Pentylphenylboronic Acid to Enhance Affinity towards Bacteria and Dopamine

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