Investigation of Somatic Embryogenesis for in Vitro Cultures of Date Palm (Phoenix Dactylifera L.)

Asemota, Omorefe; Eke, Chukwuemeka R.; Emoghene, B.O.; Aisueni, N. O.; Adetunji, Oluwaseyi Adegoke; Gidado, Rose; Solomon, Babette

doi:10.17660/actahortic.2010.882.25

Cited by 3 publications

(4 citation statements)

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“…The explants were induced in MS medium supplemented with 100 mg/L 2,4-D, 3 mg/L kinetin and 1 mg/L BAP to achieve production of callus. The current results were very similar to those of Asemota et al (2010); Aslam and Khan (2009); Al-Khateeb (2008);El-Din et al (2007); Eshraghi et al (2005); Eke et al (2005); Zaid (2003); Daguin and Letoutze (1988) and Tisserat (1983) who used 100 mg/L 2,4-D and 3 mg/L 2iP added to the culture medium. The ratios of auxin to cytokinin were similar, with the difference being kinetin and BAP were used in the current study.…”

Section: Callus Inductionsupporting

confidence: 89%

“…The most extensively used medium for date palm tissue culture is MS medium. However, a plant growth regulator (PGR) is often added to the MS medium, such as 100 mg/L 2,4-D and 3 mg/L 2iP (Asemota et al, 2010;Al-Khateeb, 2008;El-Din et al, 2007;Eshraghi et al, 2005 andTisserat, 1983). Adding 1.5 g/L activated charcoal to the medium avoided browning during initiation and callus induction (Al-Khalifah et al, 2013).…”

Section: Callus Inductionmentioning

confidence: 99%

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Micropropagation of “KL1” date palm (Phoenix dactylifera L.)

2020

ANRES

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Section: Callus Inductionsupporting

confidence: 89%

Section: Callus Inductionmentioning

confidence: 99%

Micropropagation of “KL1” date palm (Phoenix dactylifera L.)

2020

ANRES

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“…Most researchers suggest 2, 4-dichlorophenoxy acetic acid (2, 4-D) as the most effective auxin to induce embryogenic callus (Al-Khayri and Naik, 2017). Asemota et al, (2010) reported the use of 100mg/l NAA or 2, 4-D and 3mg/l 2ip to induce embryogenic callus from the meristem and leaf of date palm explants.…”

Section: Discussionmentioning

confidence: 99%

Effects Of Different Sterilization Regimes & Growth Regulators On Micropropagation Of Female Date Palm (<i>Phoenix dactylifera</i> L.)

Emoghene¹,

Idu²,

Eke³

et al. 2020

Nig. J. Biotechnol

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The success of in vitro culture techniques is always hampered by microbial contamination. The present study was carried out to develop an efficient protocol for date palm explants sterilization for successful somatic embryos induction and plantlets formation of some date palm varieties. The shoot tips were treated with different sterilizing agents at different concentrations and durations of exposure. The use of ethanol (70%), sodium hypochlorite (3.5% & 70%) and mercuric chloride (0.2%) with or without addition of Tween-20 had different effects on decontamination of the date palm explants. The percentage of explants contaminated with bacteria for sterilants 1, 2 and 4 was 18.8%, 6.3% and 6.3% respectively while 25%, 37.5%, 31.25% and 6.25% were contaminated with fungi for sterilants 1, 2, 3 and 4 respectively. Under the conditions used, a combination of antioxidants (Citric and Ascorbic acids at 100mg/l), 0.2% mercuric chloride and 3.5% sodium hypochlorite solution with 3 drops/100ml of Tween-20 helped in the reduction of chlorosis, contamination and die-back in the shoot tip explants. The explants were further cultured in appropriate media for callus initiation and subsequent somatic embryo induction. Optimal embryogenic callus was obtained from the shoot explant of sterilant number 4 which had the minimal contamination and die-back of all the cultures. After 3 subcultures, the somatic embryos formed were multiplied for shoot development. From this study, we established that the use of appropriate surface sterilant at suitable concentration and duration of exposure of date palm explant to it is indispensable for maximum responses of in vitro cultures. Keywords: Date palm, Microbial contamination, Sterilizing agents, in vitro, Somatic embryos

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“…Date is a major fruit crop of the arid climate region in the countries of the Middle East and North Africa. The total production of date palm fruit ranges between 2.5 and 4 million tons per year in the world 1 . Date palms are generally propagated by separating the offshoots produced by individual trees.…”

Section: Introductionmentioning

confidence: 99%